[1]刘红,李俊,黄成,等.瞬时受体电位M7通道蛋白对大鼠肝星状细胞凋亡的影响[J].中国药理学通报,2012,(04):486.
 LIU Hong,LI Jun,HUANG Cheng,et al.The effects of transient receptor potential melastaln 7 channel protein on apoptosis of rat hepatic stellate cells[J].Chinese Pharmacological Bulletin,2012,(04):486.
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瞬时受体电位M7通道蛋白对大鼠肝星状细胞凋亡的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2012年04期
页码:
486
栏目:
论著
出版日期:
2012-04-25

文章信息/Info

Title:
The effects of transient receptor potential melastaln 7 channel protein on apoptosis of rat hepatic stellate cells
作者:
刘红李俊黄成黄艳周德喜
安徽省天然药物活性研究重点实验室、安徽医科大学药学院,安徽 合肥230032
Author(s):
LIU HongLI JunHUANG ChengHUANG YanZHOU Dexi
Anhui Key Laboratory of Bioactivity of Natural Products,School of Pharmacy, Anhui Medical University,Hefei230032,China
关键词:
瞬时受体电位M7通道肝星状细胞细胞凋亡siRNACaspase3Bid
Keywords:
transient receptor potential melastain 7hepatic stellate cellcell apoptosissiRNACaspase3Bid
分类号:
R332;R32247;R32925;R5752022; R9776
摘要:
目的通过转染特异性siRNA,下调瞬时受体电位M7通道蛋白(TRPM7)在大鼠肝星状细胞系HSCT6中的表达,研究其对HSCT6凋亡的影响及内在机制。方法利用LipofectamineTM 2000将特异性TRPM7siRNA转染到HSCT6内,分别运用流式细胞术检测HSCs凋亡变化;Western blot检测TRPM7、Caspase3蛋白的表达;RTPCR检测TRPM7,Caspase3、Bid mRNA的表达。结果TRPM7siRNA 明显抑制TRPM7 mRNA和蛋白的表达。与正常组或对照组相比,转染组细胞凋亡率、凋亡蛋白Caspase3及凋亡基因Bid的表达均明显上调。结论特异性TRPM7siRNA能明显抑制TRPM7表达,诱导HSCs凋亡,其机制可能与其诱导凋亡基因Bid表达上调有关。
Abstract:
AimTo investigate the effects of downexpression of transient receptor potential melastatin 7(TRPM7) and its internal mechanisms on apoptosis through transfecting specific siRNA in rat hepatic stellate cell lines HSCT6.MethodsSpecific TRPM7siRNA was transfected by LipofectamineTM 2000. Cell apoptosis was measured by Flow Cytometry. TRPM7 and Caspase3 protein expression were analyzed by Western blot;TRPM7,Caspase3 and Bid mRNA expression were evaluated by RTPCR.ResultsSuppression TRPM7 expression by siRNA significantly increased the apoptosis of HSCs,the expression of Caspase3 protein and Bid mRNA compared with normal or control.ConclusionsSpecific TRPM7siRNA not only inhibits the expression of TRPM7 but also induces apoptosis of HSCs. The enhanced gene expression of Bid may contribute to HSCs apoptosis.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-12-08,修回日期:2012-01-09
基金项目:国家自然科学基金资助项目(No 81072686, 30873081, 81102493);教育部博士学科点新教师
基金项目(No 20103420120001)
作者简介:刘红(1985-),女,硕士,研究方向:抗炎免疫药理学,Email:aydlhlvsk@163.com;
更新日期/Last Update: 2012-04-25