[1]李荣,李俊,胡成穆,等.橙皮苷对大鼠佐剂性关节炎的治疗作用及机制[J].中国药理学通报,2008,(04):0.
 LI Rong,LI Jun,HU Cheng mu,et al.Therapeutic effect of hesperidin on adjuvant arthritis in rats and its mechanisms[J].Chinese Pharmacological Bulletin,2008,(04):0.
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橙皮苷对大鼠佐剂性关节炎的治疗作用及机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2008年04期
页码:
0
栏目:
论著
出版日期:
2008-04-25

文章信息/Info

Title:
Therapeutic effect of hesperidin on adjuvant arthritis in rats and its mechanisms
作者:
李荣李俊胡成穆张磊姜辉
安徽医科大学药学院,安徽 合肥230032
Author(s):
LI RongLI Jun HU Chengmu ZHANG Lei JIANG Hui
School of Pharmacy, Anhui Medical University, Hefei230032,China
关键词:
橙皮苷佐剂性关节炎免疫调节
Keywords:
hesperidin adjuvant arthritis immunomodulation
分类号:
R332;R 2841;R 32924;R 39312;R 593220531
文献标志码:
A
摘要:
目的研究橙皮苷(hesperidin,HDN)对佐剂性关节炎(AA)大鼠的治疗作用及部分机制。方法用弗氏完全佐剂(FCA)诱导大鼠AA模型;足容积法测量继发侧足肿胀度;MTT法检测刀豆蛋白(ConA)和脂多糖(LPS)诱导的脾淋巴细胞增殖反应;放免法测定脾淋巴细胞产生IL2的水平以及腹腔巨噬细胞(PMΦ)IL1,IL6和TNFα的水平;酶联免疫吸附测定法(ELISA)测定PMΦ产生IL10的水平。结果致炎后d 12,大鼠继发性关节炎出现,同时灌胃给予不同剂量的HDN(40、80、160 mg·kg-1),连续12 d,从致炎后d 20开始,HDN(80、160 mg·kg-1)对AA大鼠继发性炎症有明显抑制作用;HDN各剂量可不同程度地纠正AA大鼠低下的脾淋巴细胞增殖反应和脾细胞IL2的产生,降低AA大鼠PMΦ产生过高的IL1,IL6和TNFα,同时上调AA大鼠PMΦ低下的IL10水平。结论HDN对AA大鼠继发性炎症具有治疗作用,其作用机制可能与调节机体异常的免疫功能和维持细胞因子网络平衡有关。
Abstract:
AimTo study the therapeutic effect of hesperidin (HDN) on adjuvant arthritis (AA) in rats and its mechanisms.MethodsFreund′s complete adjuvant (FCA) was used to induce AA in rats. Secondary paw swelling of AA rats was measured with volume meter. Splenic lymphocyte proliferation response induced by concanavalin A (ConA) or lipopolysaccharide (LPS) was examined with MTT assay. IL2 production of splenic lymphocytes and IL1, IL6,TNFα productions of peritoneal macrophage (PMΦ) were determined by radioimmunity assay.IL10 production of PMΦ was estimated by enzyme linked immunosorbent assay (ELISA).ResultsThe secondary inflammation of AA rats appeared on the 12th day after injection of FCA. At the same time (d 12), HDN (40,80,160 mg·kg-1,×12 d) were given to AA rats by intragastric administration. It was found that HDN(80, 160 mg·kg-1) could significantly inhibit the secondary paw swelling of AA rats from the 20 th day.The suppressed lymphocyte proliferation and IL2 production of splenic lymphocytes in AA rats were reversed by treatment with HDN. Meanwhile,HDN could remarkably downregulate IL1,IL6,TNFα productions of PMΦ and upregulate IL10 production of PMΦ.ConclusionsThe results suggested that HDN had therapeutical effect on AA rats. Its mechanisms may be related to adjusting abnormal immune function in AA rats and keeping the balance of cytokine network.

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备注/Memo

备注/Memo:
收稿日期:2007-11-30,修回日期:2008-01-12基金项目:安徽省高校青年教师资助项目(No 2007jq1080)作者简介:李荣(1979-),男,博士生,讲师,研究方向:抗炎免疫药理学,Email:aydlirong@163.com;李俊(1960-),男,教授,博士生导师,研究方向:抗炎免疫药理学、临床药理学,通讯作者,Email:Lijun@ahmu.edu.cn
更新日期/Last Update: 2008-04-15