[1]王乐,王兰兰,曹宇.TRPV1对LPS致热大鼠体温及下丘脑中Ca2+浓度和cAMP含量的影响[J].中国药理学通报,2009,(01):0.
 WANG Le,WANG Lan lan,CAO Yu.Effects of TRPV1 on LPSinduced fever and the content of Ca2+ and cAMP in hypothalamus in rats[J].Chinese Pharmacological Bulletin,2009,(01):0.
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TRPV1对LPS致热大鼠体温及下丘脑中Ca2+浓度和cAMP含量的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2009年01期
页码:
0
栏目:
论著
出版日期:
2009-01-25

文章信息/Info

Title:
Effects of TRPV1 on LPSinduced fever and the content of Ca2+ and cAMP in hypothalamus in rats
作者:
王乐王兰兰曹宇
中国医科大学基础医学院生理学教研室,辽宁 沈阳110001
Author(s):
WANG LeWANG LanlanCAO Yu
Dept of Physiology,College of Basic Medical Sciences,China Medical University,Shenyang110001,China
关键词:
脂多糖发热TRPV1capsazepinecAMP[Ca2+]i
Keywords:
lipopolysaccharide (LPS)fevertransient receptor potential vanilloid 1 (TRPV1)capsazepinecyclic adenosine monophosphate (cAMP)[Ca2+]i
分类号:
R332;R 32281;R 3481;R 3424
文献标志码:
A
摘要:
目的探讨TRPV1在脂多糖(LPS)致大鼠发热过程中的作用和机制。方法48只♂ SD大鼠随机分为4组,正常对照组(N)、capsazepine组(CPZ)、LPS组(LPS)和capsazepine+LPS组(CPZ+LPS)。连续观察体温变化;在发热高峰期通过颈椎脱臼迅速处死动物,以Fura2/AM为荧光指示剂,测定下丘脑细胞内[Ca2+]i;应用放射免疫法检测下丘脑中cAMP含量的变化。结果① 各组体温变化最大值(ΔTmax)由高至低顺序为:CPZ+LPS组>LPS组>CPZ组>N组;其中,CPZ+LPS组与LPS组比较,ΔT(300480 min期间)及体温反应指数TRI8均增高(P<001);② 在发热高峰期,CPZ+LPS组[Ca2+]i明显低于其它3组(P<001);③ CPZ+LPS组cAMP含量与其它组比较增多(P<001)。结论capsazepine可能通过阻断下丘脑TRPV1通道,改变细胞内钙离子浓度,进而影响LPS性发热过程。
Abstract:
AimTo observe the effect of TRPV1 on the febrile response in fever rat,and further to investigate TRPV1 action in thermoregulation and the possible central mechanism.MethodsFortyeight rats were divided randomly into 4 groups:the control group (N),capsazepine group (CPZ),LPSfeverish group (LPS),the capsazepine+LPSfeverish group (CPZ+LPS).Changes in body temperature were continually observed;rats were put to death immediately at the peak of fever,[Ca2+]i was measured by the technique of Fura2/AM calcium ions fluorescence indicator;the content of cAMP in hypothalamus was measured by radioimmunoassay.Results① The sequence of the maximum change of temperature (ΔTmax) from high to low:group CPZ+LPS>group LPS>group CPZ>group N; ΔT (300~480 min) and TRI8 of group CPZ+LPS was significantly higher than that of group LPS (P<001);② At the peak of fever, [Ca2+]i of group CPZ+LPS was significantly lower than that of the other three(P<001);③ The content of cAMP in hypothalamus of group CPZ+LPS was the highest (P<001).ConclusionIt was possible that capsazepine changed intra cellular [Ca2+]i by blocking TRPV1 channel in hypothalamus,and then affected LPSinduced fever.

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备注/Memo

备注/Memo:
收稿日期:2008-07-17, 修回日期:2008-09-19基金项目:国家自然科学基金资助项目(No 30270198);高等学校博士学科点专项科研基金资助项目(No 2006159004)作者简介:王乐(1983-),女,硕士生,研究方向:体温调节,Email:rachel8228@163.com;曹宇(1963-),女,教授,博士生导师,研究方向:体温生理,通讯作者,Tel:024232566665301,Email:caoy595@sina.com
更新日期/Last Update: 2009-01-25