[1]江国林,李俊,黄艳,等.丝裂原活化蛋白激酶通路对慢支大鼠肺泡巨噬细胞产生TNFα的影响及枇杷叶三萜酸的作用机制[J].中国药理学通报,2009,(05):0.
 JIANG Guo lin,LI Jun,HUANG Yan,et al.Effect of MAPK signal transduction pathway on the expression of TNFα in alveolar macrophage of chronic bronchitis rats and the target of TAL[J].Chinese Pharmacological Bulletin,2009,(05):0.
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丝裂原活化蛋白激酶通路对慢支大鼠肺泡巨噬细胞产生TNFα的影响及枇杷叶三萜酸的作用机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2009年05期
页码:
0
栏目:
论著
出版日期:
2009-05-25

文章信息/Info

Title:
Effect of MAPK signal transduction pathway on the expression of TNFα in alveolar macrophage of chronic bronchitis rats and the target of TAL
作者:
江国林李俊黄艳黄晓晖朱鹏里伍长娟
安徽医科大学药学院,安徽 合肥230032
Author(s):
JIANG GuolinLI JunHUANG YanHUANG XiaohuiZHU PengliWU Changjuan
School of Pharmacy,Anhui Medical University,Hefei230032,China
关键词:
枇杷叶三萜类慢性支气管炎肺泡巨噬细胞肿瘤坏死因子MAPK
Keywords:
Loquat.leaf triterpenes chronic bronchitis alveolar macrophageTNFαMAPK
分类号:
R332;R 2841
文献标志码:
A
摘要:
目的探究丝裂原活化蛋白激酶(MAPK)信号转导通路对慢性支气管炎(CB)大鼠肺泡巨噬细胞(AM)中肿瘤坏死因子α(TNFα)生成的影响及枇杷叶三萜酸(TAL)的作用环节。为TAL治疗慢支的抗炎作用机制提供进一步的实验依据。方法以AM为实验平台分别给予MAPK 3条通路(ERK,p38,JNK)的特异性阻滞剂,用RTPCR和Western blot的方法来分别检测CB大鼠AM中TNFα的mRNA及蛋白的表达水平。并采用析因实验设计,ELISA法检测AM培养上清中的TNFα的水平。结果RTPCR和Western blot的结果均显示ERK特异性阻滞剂(PD98059)和p38特异性阻滞剂(SB203580)对CB大鼠AM中TNFα的表达有明显的抑制作用。析因分析结果表明TAL、特异性阻滞剂(PD98059和SB203580)及其交互作用项对TNFα含量的影响存在统计学意义。结论TAL对TNFα的抑制作用可能与其作用于ERK和p38通路有关,这可能是TAL治疗与防治慢支的主要作用环节之一。
Abstract:
AimTo investigate the effect of MAPK signal transduction pathway on the expression of TNFα in AM(alveolar macrophage) of CB(chronic bronchitis) rats, meanwhile, to initially discuss the possible regulated target of TAL(triterpene acids of loquat.leaf),to explain the mechanism about antiinflammatory action of TAL on CB rats.MethodsThe depurated AM was incubated with the special inhibitors of MAPK signal transduction pathway respectively (ERK,p38 or JNK) and the expression of TNFα was observed by RTPCR and Western blot. Using factorial design,TNFα level in AM culture supernatant was detected by ELISA.ResultsThe special inhibitors of ERK(PD98059) and p38(SB203580) could change the expression of TNFα in AM of CB rats apparently. Factorial analysis shows there is statistical significance between TAL,PD98059/SB203580 and mixture of them.ConclusionsTAL reducing the expression of TNFα may be related with the inhibition of ERK and p38 signal transduction pathway. It may be one of the mechanisms by which TAL has the preventive and therapeutic effects on chronic bronchitis.

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备注/Memo

备注/Memo:
收稿日期:2008-12-27,修回日期:2009-02-12基金项目:国家自然科学基金资助项目(No 30572355);安徽省高等学校优秀青年人才基金重点项目(No 2009SQRZ049ZD);安徽省自然科学基金资助项目(No 090413134)作者简介:江国林(1983-),女,硕士,研究方向:临床药理学,Email:guoguoyaya0619@yahoo.com.cn;李俊(1960-),男,博士,教授,博士生导师,研究方向:抗炎免疫药理学及分子药理学,通讯作者,Email:lijun@ ahmu.edu.cn
更新日期/Last Update: 2009-05-25