[1]吴会生,郭培培,尚游,等.氟比洛芬酯预处理对大鼠全脑缺血/再灌注时脑组织TXA2/PGI2的影响[J].中国药理学通报,2009,(09):0.
 WU Hui sheng,GUO Pei pei,SHANG You,et al.Effect of flurbiprofen axetil pretreatment on TXA2/PGI2 of brain tissue in rats subjected to global cerebral ischemiareperfusion injury[J].Chinese Pharmacological Bulletin,2009,(09):0.
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氟比洛芬酯预处理对大鼠全脑缺血/再灌注时脑组织TXA2/PGI2的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2009年09期
页码:
0
栏目:
论著
出版日期:
2009-09-25

文章信息/Info

Title:
Effect of flurbiprofen axetil pretreatment on TXA2/PGI2 of brain tissue in rats subjected to global cerebral ischemiareperfusion injury
作者:
吴会生1郭培培2尚游2刘丹彦1安民1雷蕾1邹磊1
1.重庆医科大学附属第一医院麻醉科,重庆400016;、2.华中科技大学同济医学院附属协和医院麻醉科,湖北 武汉430022
Author(s):
WU Huisheng1GUO Peipei2SHANG You2LIU Danyan1AN Min1LEI Lei1ZOU Lei1
1.Dept of Anesthesiology,the First Affiliated Hospital of Chongqing Medical University,Chongqing400016,China;2.Dept of Anesthesiology,Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan430022,China
关键词:
氟比洛芬 脑缺血/再灌血栓素A2前列环素预处理大鼠
Keywords:
flurbiprofencerebral ischemiareperfusionTXA2PGI2preconditioningrat
分类号:
R332;R 32281;R 34921
文献标志码:
A
摘要:
目的探讨氟比洛芬酯预处理对大鼠全脑缺血/再灌注损伤的影响。方法72只♂SD大鼠,体重250350 g,随机分为4组(n=18):假手术组(SH组)、模型组(Model组)、氟比洛芬酯5 mg·kg-1组(F5组)和氟比洛芬酯10 mg·kg-1组(F10组)。采用夹闭双侧颈总动脉20 min合并低血压法建立全脑缺血/再灌注(I/R)模型。F5组和F10组分别于缺血前15 min静注氟比洛芬酯5 mg·kg-1+空白乳剂0.5 ml·kg-1、氟比洛芬酯10 mg·kg-1。于再灌注6、24、72 h时行神经功能缺陷评分(NDS)、 HE染色观察海马CA1区细胞损伤情况、放射免疫法测定额区皮质血栓素A2(TXA2)和前列环素(PGI2)含量。结果氟比洛芬酯5 mg·kg-1组(F5组)或氟比洛芬酯10 mg·kg-1组(F10组)预处理可明显减轻大鼠全脑缺血/再灌注后海马CA1区损伤,提高神经功能缺陷评分(NDS),降低脑组织中TXA2含量和TXA2/PGI2值,且F10组较F5组海马CA1区损伤更轻。结论氟比洛芬酯预处理可减轻全脑缺血/再灌注损伤,其机制可能与降低TXA2值和TXA2/PGI2值有关。
Abstract:
AimTo investigate the effect of flurbiprofen axetil pretreatment on global cerebral ischemiareperfusion injury in rats.Methods72 Adult male SpragueDawley rats weighing 250350 g were randomly divided into four groups: sham group(SH), global cerebral ischemiareperfusion model group(Model), flurbiprofen axetil 5 mg·kg-1 group(F5)and flurbiprofen axetil 10 mg·kg-1 group(F10).Global cerebral ischemia was induced by 20 min occlusion of bilateral common carotid arteries combined with hypotension.In group F5 and F10 flurbiprofen axetil 5 mg·kg-1+blank emulsion 05 ml·kg-1 and 10 mg·kg-1 was injected iv at 15 min before ischemia respectively. The neurological deficit(NDS) was scored (0:normal,100:brain death) at 6,24 and 72 h of reperfusion by the same observer.Pathological changes in CA1 region of hippocampus were observed at 6, 24 and 72 h of reperfusion through HE staining. Radioimmunoassay technique was used to determine TXA2 and PGI2 content of the brain tissue at 6,24 and 72 h of reperfusion.ResultsPretreatment with flurbiprofen axetil 5 mg·kg-1 or flurbiprofen axetil 10 mg·kg-1 significantly reduced histopathological injury in CA1 region of hippocampus, improved neurological deficit score(NDS),reduced TXA2 content and TXA2/PGI2 of the brain tissue.Moreover,the effect of reducing histopathological injury in CA1 region of hippocampus in group F10 was better than that of group F5.ConclusionPretreatment with flurbiprofen axetil can protect against global cerebral ischemia through inhibition of TXA2 content and TXA2/PGI2 of the brain tissue.

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备注/Memo

备注/Memo:
收稿日期:2009-05-12,修回日期:2009-06-22基金项目:国家自然科学基金资助项目(No 30700784)作者简介:吴会生(1982-),男,硕士,住院医师,研究方向:麻醉与脑保护,Email:wggwwggw82@sina.com; 刘丹彦(1964-),女,副教授,硕士生导师,研究方向:麻醉与脑保护,通讯作者,Tel:02389011069,Email:liudanyan418@sina.com
更新日期/Last Update: 2009-09-25