[1]孟晶,翟云鹏,段世明,等.氯胺酮抗惊厥的作用机制[J].中国药理学通报,2009,(09):0.
 MENG Jing,ZHAI Yun peng,DUAN Shi ming,et al.Study on anticonvulsant mechanism of ketamine[J].Chinese Pharmacological Bulletin,2009,(09):0.
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氯胺酮抗惊厥的作用机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2009年09期
页码:
0
栏目:
论著
出版日期:
2009-09-25

文章信息/Info

Title:
Study on anticonvulsant mechanism of ketamine
作者:
孟晶1翟云鹏2段世明1马涛1戴体俊1
徐州医学院1.江苏省麻醉学重点实验室、2.药理学教研室, 江苏 徐州221002
Author(s):
MENG Jing1 ZHAI Yunpeng2DUAN Shiming1MA Tao1DAI Tijun1
1.Jiangsu Province Key Laboratory of Anesthesiology;2.Dept of Phamacology, Xuzhou Medical College,Xuzhou Jiangsu221002,China
关键词:
氯胺酮 抗惊厥 大脑皮层 机制Na+K+ATP酶Ca2+ATP酶NOS酶活性
Keywords:
ketamine anticonvulsion cerebral cortex mechanismNa+K+ATPaseCa2+ATPaseNOSase
分类号:
R332;R 32281
文献标志码:
A
摘要:
目的观察氯胺酮对惊厥小鼠不同脑区Na+,K+ATP酶、Ca2+ATP酶以及NOS酶活性的影响,探讨氯胺酮抗惊厥作用的中枢机制。方法昆明种小鼠随机分成空白组、生理盐水(NS)组、氯胺酮25 mg·kg-1(KetⅠ)和50 mg·kg-1(KetⅡ)组。空白组直接断头取脑。其余各组分别ip相应药物,5 min后ip士的宁15 mg·kg-1诱发惊厥,观察惊厥小鼠行为学变化,并于给士的宁30 min后断头,用分光光度计法测定皮层额、顶、枕脑区的Na+,K+ATP 酶、Ca2+ATP酶和NOS酶活性。结果氯胺酮组明显降低动物死亡率,KetⅡ组惊厥持续期较KetⅠ组明显缩短。与空白组相比,NS组和KetⅠ组Na+,K+ATP酶、Ca2+ATP酶活性均有降低,KetⅡ组顶、枕区Na+,K+ATP和Ca2+ATP酶活性维持在正常水平;KetⅡ组TNOS酶活性降低约1/3(P<005),各组对iNOS活性均无影响。结论氯胺酮抗惊厥作用机制可能与增加大脑皮层顶枕区的Na+,K+ATP和Ca2+ATP酶活性、降低cNOS酶活性有关。
Abstract:
AimTo observe the effects of ketamine on Na+,K+ATPase, Ca2+ATPase and NOSase activity in different cerebral cortex in convulsive mice.MethodsThe mice were randomly divided into blank group, normal saline(NS) group and ketamine 25 mg·kg-1 (KetⅠ),50 mg·kg-1(KetⅡ) group. The animals of blank group were killed directly.Convulsion was induced by intraperitoneally(ip) strychnine(15 mg·kg-1) in other groups,and correspond drugs were administered ip before five minutes.Action variety of mice was observed. Animals were killed on 30 minutes after strychnine injection.The activity of Na+,K+ATPase, Ca2+ATPase, TNOSase and iNOSase were assessed by spestrophotometric analysis in different cerebral cortex(forehead, parietal and occipital area).ResultsKetamine group could decrease mortality completely. The duration of tonic state in KetⅡ group was significantly shorter than that in KetⅠgroup.Compared with blank group, Na+,K+ATPase and Ca2+ATP ase activities were decreased in the group of NS and KetⅠ, and recovered normal level in the group of KetⅡ at parietal and occipital area. TNOS ase activity was decreased by 1/3 in KetⅡ group(P<005), and iNOSase activity was not affected in all groups.ConclusionThe increase of Na+,K+ATPase, Ca2+ATP ase activity in parietal and occipital area and the decrease of cNOSase activity may be involved in the anticonvulsant mechanism of ketamine.

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备注/Memo

备注/Memo:
收稿日期:2009-05-11,修回日期:2009-06-23基金项目:国家自然科学基金资助项目(No 39970715,30471657,30872432);江苏省高校自然科学基础研究资助项目(No 07KJD310219)作者简介:孟晶(1976-),女,硕士,讲师,研究方向:全麻原理,Tel:051683262155,Email:mengjingmz@sohu.com;戴体俊(1944-),男,教授,研究方向:全麻原理,Tel:051681789500,Email:daitijun@163.com
更新日期/Last Update: 2009-09-25