[1]吴宝明,李俊,吕雄文,等.蜂毒素对SGC7901细胞生长及G2/M期阻滞的影响[J].中国药理学通报,2010,(02):222-225.
 WU Bao ming,LI Jun,L Xiong wen,et al.Effects of Melittin on the growth and G2/M phase arrest in SGC7901 cells[J].Chinese Pharmacological Bulletin,2010,(02):222-225.
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蜂毒素对SGC7901细胞生长及G2/M期阻滞的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2010年02期
页码:
222-225
栏目:
论著
出版日期:
2010-02-25

文章信息/Info

Title:
Effects of Melittin on the growth and G2/M phase arrest in SGC7901 cells
作者:
吴宝明李俊吕雄文金涌赵斌黄艳王怡苹楼蓉王朋景
安徽医科大学药学院,安徽 合肥230032
Author(s):
WU BaomingLI JunL XiongwenJIN YongZHAO BinHUANG YanWANG YipingLOU RongWANG Pengjing
College of Pharmacy,Anhui Medical University,Heifei230032,China
关键词:
蜂毒素SGC7901细胞细胞增殖细胞周期阻滞CylinB1CDK1CDc25c
Keywords:
melittinSGC7901 cellproliferationcell cycle arrestCylinB1CDK1CDc25c
分类号:
R 97919;R 32244;R 3292
文献标志码:
A
摘要:
目的研究蜂毒素(Melittin)对胃上皮癌细胞SGC7901细胞生长及细胞周期阻滞的影响。方法采用罗丹明B(SRB)染色法观察Melittin对SGC7901细胞株生长曲线的影响;用流式细胞仪检测Melittin对该细胞周期阻滞的影响;RTPCR检测细胞周期相关基因mRNA的表达。结果Melittin(1、2、4、8×10-3μg·L-1)体外给药24 h,能抑制SGC7901细胞的增殖活性(P<005 or P<001);Melittin(4、8×10-3 μg·L-1)作用SGC7901 24 h后,能明显阻滞该细胞株于G2/M期;并且降低G2/M期相关基因CylinB1CDK1Cdc25c mRNA的表达。结论Melittin具有抑制胃上皮癌细胞SGC7901增殖的作用,其机制可能与干扰该细胞G2/M期相关基因的转录有关。
Abstract:
AimTo explore the effects of the Melittin on growth and cell cycle of SGC7901 cells.MethodsGrowth inhibition effect of Melittin was evaluated using SRB in SGC7901 cells in vitro;Melittin induced cell cycle arrest was investigated using flow cytometry assay;reverse transcription PCR(RTPCR)was used to detect the associated protein mRNA of cell cycle.ResultsProliferation activity of SGC7901 cells was inhibited after treatment with Melittin(1,2,4,8,16,32×10-3 μg·L-1)(P<005 or P<001)for 24 h;Flowcytometry analysis revealed that SGC7901 cells accumulated in the G2/M phase after treatment with Melittin(4,8×10-3 μg·L-1)for 24 h;the expression of CylinB1,CDK1 and Cdc25c mRNA were decreased.ConclutionsProliferation activity of SGC7901 cells was inhibited by Melittin,which may be related to the inhibitory effect of Melittin on associated protein transcription in the G2/M stage of SGC7901 cells.

参考文献/References:

[1]金懋林.胃癌药物治疗研究新进展[J].临床药物治疗杂志,2006,4(2):13-6.[1]Jin M L.New progress of drug therapy in gastric cancer[J].Clin Med J,2006,4(2):13-6.
[2]Moon D O,Park S Y,Lee K J,et al.Bee venom and melittin reduce proinflammatory mediators in lipopolysaccharidestimulated BV2 microglia[J].Int Immunopharmacol,2007,7(8):1092-101.
[3]Moon D O,Park S Y,Choi Y H,et al.Melittin induces Bcl2 and caspase3dependent apoptosis through downregulation of Akt phosphorylation in human leukemic U937 cells[J].Toxicon,2008,51:112-20.
[4]Vichai V,Kirtikara K.Sulforhodamine B colorimetric assay for cytotoxicity screening [J].Nat Protoc,2006,1(3):1112-6.
[5]韩家凯,焦东晓,曹建国,等.黄荆子乙酸乙酯提取物体内外对胃癌SGC7901细胞作用的研究[J].中国药理学通报,2008,24(12):1652-6.
[5]Han J K,Jiao D X,Cao J G,et al.Effects of the extract from semen viticis negundo with acetoactate on human gastric carcinoma SGC7901 cell in vitro and in vivo[J].Chin Pharmacol Bull,2008,24(12):1652-6.
[6]蒋国松,童强松,曾甫清,等.茉莉素对人神经母细胞瘤细胞株SHSY5Y的生长抑制作用及其机制研究[J].中国药理学通报,2008,24(4):431-6.
[6]Jiang G S,Tong Q S,Zeng F Q,et al.Growth inhibition effects of jasmonates on human neuroblastoma cell line SHSY5Y and its mechanisms[J].Chin Pharmacol Bull,2008,24(4):431-6.
[7]Saini S S,Chopra A K,Peterson J W.Melittin activates endogenous phospholipase D during cytolysis of human monocytic leukemia cell[J].Toxicon,1999,37(11):1605-19.
[8]Zhu H G,Tayeh I,Israel L,Castagna M.Different susceptibility of lung cell lines to inhibitors of tumor promotion and inducers of detterentiation[J].J Biol Regul Homeost Agengts,1991,5(2):52-8.
[9]曹亚.细胞周期与肿瘤[J].国外医学-生理、病理科学与临床分册,2002,22(2):103-5.
[9]Cao Ya.Cell cycle and tumor[J].Fore Med Sci Sect Pathophys Clin Med,2002,22(2):103-5.
[10]Abraham R T.Cell cycle checkpoint signaling through the ATM and ATR kinases[J].Genes Dev,2001,15(17):2177-96.
[11]Lavecchia A,Di Giovanni C,Novellino E.ICDC25A and B dualspecificity phosphatase inhibitors:potential agents for cancer therapy[J].Curr Med Chem,2009,16(15):1831-49.
[12]Donzelli M,Draetta G F.Regulating mammalian checkpoints through Cdc25 inactivation[J].EMBO,2003,4(7):671-7.

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备注/Memo

备注/Memo:
收稿日期:2009-11-02,修回日期:2009-12-28基金项目:安徽省自然科学基金资助项目(No 90413140);安徽高校省级优秀青年人才基金资助(No 2009SQR2043);安徽省高校青年教师科研资助项目(No 2008jq1067)作者简介:吴宝明(1980-),男,硕士,Email:anyi7955@ 163.com;李俊(1960-),男,教授,博士生导师,研究方向:临床药理学、抗炎免疫药理学、药代动力学,通讯作者,Tel:05515161001,Email:lijun@ahmu.edu.cn
更新日期/Last Update: 2010-02-25