[1]周倩,李俊,王婷玉,等.豹皮樟总黄酮对胶原性关节炎大鼠腹腔巨噬细胞产生细胞因子及其免疫功能的影响[J].中国药理学通报,2010,(03):353-357.
 ZHOU Qian,LI Jun,WANG Ting yu,et al.Effect of total flavonids of Litsea coreana Leve on cytokines production and immunity of peritoneal macrophage from collageninduced arthritis[J].Chinese Pharmacological Bulletin,2010,(03):353-357.
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豹皮樟总黄酮对胶原性关节炎大鼠腹腔巨噬细胞产生细胞因子及其免疫功能的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2010年03期
页码:
353-357
栏目:
论著
出版日期:
2010-03-25

文章信息/Info

Title:
Effect of total flavonids of Litsea coreana Leve on cytokines production and immunity of peritoneal macrophage from collageninduced arthritis
作者:
周倩李俊王婷玉王小华
安徽医科大学药学院,安徽 合肥230032
Author(s):
ZHOU QianLI JunWANG TingyuWANG Xiaohua
College of Pharmacy,Anhui Medical University,Hefei230032,China
关键词:
豹皮樟黄酮关节炎巨噬细胞免疫吞噬功能
Keywords:
Litsea coreana Leveflavonoidarthritismacrophageimmunityphagocytosis
分类号:
R332;R 2841;R 39212
文献标志码:
A
摘要:
目的研究豹皮樟总黄酮(total flavonoids of Litsea coreana Leve,TFLC)对胶原性关节炎大鼠(collageninduced arthritis,CIA)腹腔巨噬细胞(peritoneal macrophage,PMΦ)产生细胞因子及其免疫功能的影响。方法Ⅱ型胶原蛋白(collagen Ⅱ,CII)和氟氏完全佐剂(CFA)诱导大鼠胶原性关节炎(CIA)模型,采用体重、足爪肿胀及病理组织学变化等指标评价TFLC对CIA大鼠的治疗作用;无菌取PMΦ,体外用药(TFLC 005、05、5 mg·L-1),采用小鼠胸腺细胞增殖法检测CIA大鼠PMΦ分泌白细胞介素1(interleukin1,IL1)的水平,小鼠脾淋巴细胞增殖法检测CIA大鼠脾细胞分泌白细胞介素2(interleukin2,IL2)的水平,放射免疫测定法和反转录RTPCR法分别检测CIA大鼠PMΦ中肿瘤坏死因子α(tumor necrosis factorα,TNFα)的分泌和表达,中性红法测定CIA大鼠PMΦ的吞噬功能。结果TFLC(50、100、200 mg·kg-1)能抑制关节肿胀,增加CIA大鼠体重,改善膝关节病理损伤。TFLC(005、05、5 mg·L-1)可降低 CIA大鼠PMΦ过强的吞噬功能,下调脾细胞IL2的分泌水平和腹腔巨噬细胞IL1的分泌水平,同时抑制PMΦ TNFα的分泌水平以及TNFα mRNA的表达。结论TFLC对CIA大鼠关节炎具有明显抑制作用,其抗炎和免疫抑制作用与其降低CIA大鼠PMΦ过强的吞噬功能,抑制细胞因子的分泌和表达有关。
Abstract:
AimTo investigate the effect of total flavonids of Litsea coreana Leve(TFLC)on cytokines production and immunity of peritoneal macrophage(PMΦ)from collageninduced arthritis(CIA).MethodsCIA was induced with Collagen II and Feund's complete adjuvant.The effect of TFLC on CIA was evaluated by body weight,hind paw swelling and histopathological changes.Interleukin1(IL1)in PMΦ of CIA rats was measured by mouse thymocyte proliferation method.Interleukin2(IL2)in splenocytes of CIA rats was determined by mouse spleen lymphocyte proliferation reaction.Tumor necrosis factorα(TNFα)in the supernatant of PMΦ was measured by radioimmunoassay(RIA).And the mRNA expression of TNFα was detected by RTPCR.Phagocytosis of PMΦ was measured by neutral red method.ResultsTFLC(50,100,200 mg·kg-1 )singnificantly inhibited the paw swelling and increased body weight of CIA rats.The pathological damage of knee joint was reversed by TFLC.TFLC(005,05,5 mg·L-1)markedly reduced IL1 and IL2 in CIA rats.Meanwhile,TFLC singnificantly reduced TNFα level and inhibited the expression of TNFα mRNA in PMΦ.ConclusionTFLC has marked therapeutic effect on CIA rats,which is related to reducing the phagocytosis and inhibiting the secretion and expression of cytokines.

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备注/Memo

备注/Memo:
收稿日期:2009-12-03,修回日期:2010-01-11基金项目:安徽省科技攻关计划资助项目(No 06013134B);安徽省高等学校省级自然科学研究重点项目(No 2006kj095A);安徽省高等学校青年教师科研项目(No 2005jq1095)作者简介:周倩(1982-),女,硕士生,研究方向:临床药理学,Tel: 05515143371,Email:Zhouqian2832@163.com;李俊(1960-),男,博士,教授,博士生导师,研究方向:抗炎免疫药理学、临床药理学,通讯作者,Tel/Fax:05515161001,Email:lijun@ahmu.edu.cn
更新日期/Last Update: 2010-03-25