[1]吴晓敏,何秋霞,韩利文,等.阿司咪唑对斑马鱼心脏毒性的初步研究[J].中国药理学通报,2013,(09):1251-1254.
 WU Xiao min,HE Qiu xia,HAN Li wen,et al.Preliminary study of astemizole′s cardiotoxicity to zebrafish[J].Chinese Pharmacological Bulletin,2013,(09):1251-1254.
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阿司咪唑对斑马鱼心脏毒性的初步研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2013年09期
页码:
1251-1254
栏目:
论著
出版日期:
2013-09-10

文章信息/Info

Title:
Preliminary study of astemizole′s cardiotoxicity to zebrafish
作者:
吴晓敏12何秋霞2韩利文2袁延强2杨官娥1刘可春2
1.山西医科大学药学院,山西 太原030001;2.山东省科学院生物研究所,山东省生物传感器重点实验室,山东 济南250014
Author(s):
WU Xiaomin12 HE Qiuxia2 HAN Liwen2 YUAN Yanqiang2YANG Guane1 LIU Kechun2
1. School of Pharmacy,Shanxi Medical University,Taiyuan030001,China; 2. Biology of Institute, Shandong Academy of Science, Key Laboratory for Biosensors of Shandong Province, Jinan250014,China
关键词:
阿司咪唑斑马鱼心脏毒性心率心脏功能 药物筛选
Keywords:
astemizole zebrafish cardiotoxicity heart rate cardiac function drug screening
分类号:
R-332;R322.11;R33131;R976
文献标志码:
A
摘要:
目的研究阿司咪唑对斑马鱼的心脏毒性,为斑马鱼的心脏毒性模型提供依据。方法选择1、4、10、20 μmol·L-1的阿司咪唑观察其对72 hpf( hour post fertilization)斑马鱼的形态影响,包括畸形、心囊水肿、血细胞在心区堆积等情况,计算心率、死亡率及EC50,测量静脉窦-动脉球(SVBA)间距,以及对2 hpf斑马鱼心脏发育的影响。结果阿司咪唑处理后,斑马鱼出现出心率下降、SVBA间距增大、血细胞在心区堆积、全身无血流、心囊水肿、心脏停止跳动等中毒现象,并具有时间、剂量依赖性;同时观察到阿司咪唑对2 hpf斑马鱼的心脏发育有明显影响。结论利用阳性药物阿司咪唑探讨药物对斑马鱼心脏功能和发育方面的毒性,有助于建立基于斑马鱼的心脏毒性评价筛选体系。
Abstract:
AimTo study the cardiotoxicity of astemizole to zebrafish embryos and to supply the reliable assurance in establishing cardiotoxicity model of zebrafish. MethodsZebrafish of 72 hrs post fertilization was treated with astemizole at various concentrations including 1, 4,10, 20 and 40 μmol·L-1. The effect of astemizole on heart morphology including heart deformity, pericardial edema, hemocyte accumulation was observed. Heart rate, death rate and the EC50 were calculated. The distance between sinus venosus and bulbus arteriosus (SVBA) was measured. Zebrafish of 2 hrs post fertilization was treated with astemizole to observe its effect on heart development. ResultsDecreased heart rate, increased distance between sinus venosus and bulbus arteriosus of zebrafish embryos, hemocytes accumulation in cardiac region, whole body without blood flow, pericardial edema and the heart stoping beating emerged in zebrafish incubated with astemizole, and the toxicity presented in timeand dosedependent manner. Developmental toxicity on heart appeared in zebrafish embryos treated with astemizole.ConclusionAstemizole as a positive drug can be used to study cardiotoxicity to zebrafish both in function and development, whcih is helpful to establish cardiotoxicity assessment model with zebrafish.

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备注/Memo

备注/Memo:
收稿日期:2013-05-08,修回日期:2013-06-15 基金项目:国家自然科学基金资助项目(No 81202584) 作者简介:吴晓敏(1990-),女,硕士生,研究方向:天然药物活性筛选,Email:wuxiaomin19900201@163.com; 刘可春(1964-),男,博士,研究员,研究方向:药物筛选,通讯作者,Email:hliukch@sdas.org
更新日期/Last Update: 2013-09-10