[1]刘勇,余艳荣,彭维杰,等.吴茱萸次碱改善溶血性磷脂酰胆碱诱导的内皮细胞缝隙连接细胞间通讯功能障碍[J].中国药理学通报,2013,(11):1514-1518.
 LIU Yong,YU Yan rong,PENG Wei jie,et al.Rutaecarpine prevents the disfunction of gap junction intercellular communication induced by LPC in endothelial cells[J].Chinese Pharmacological Bulletin,2013,(11):1514-1518.
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吴茱萸次碱改善溶血性磷脂酰胆碱诱导的内皮细胞缝隙连接细胞间通讯功能障碍()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2013年11期
页码:
1514-1518
栏目:
论著
出版日期:
2013-11-11

文章信息/Info

Title:
Rutaecarpine prevents the disfunction of gap junction intercellular communication induced by LPC in endothelial cells
作者:
刘勇2余艳荣2彭维杰2况海斌1徐宏1宋培源2罗丹1
南昌大学 1.基础医学院生理学系、
2.药学院基础药理重点实验室,江西 南昌330006
Author(s):
LIU Yong2 YU Yanrong2 PENG Weijie2 KUANG Haibin1 XU Hong1 SONG Peiyuan2 LUO Dan2
1. Dept of Physiology, School of Basic Medical Sciences;
2. the Key Laboratory of Pharmacology, School of Pharmacy, Nanchang University, Nanchang330006, China
关键词:
吴茱萸次碱人脐静脉内皮细胞缝隙连接蛋白溶血性磷脂酰胆碱辣椒素受体动脉粥样硬化
Keywords:
rutaecarpine HUVEC connexin lysopbosphatidylcholine(LPC) TRPV1 atherosclerosis
分类号:
R284.1;R322.123;R329.24;R341;R543.5
文献标志码:
A
摘要:
目的探讨吴茱萸次碱(rutaecarpine,Rut)抗溶血性磷脂酰胆碱(LPC)损伤的内皮保护效应及对缝隙连接细胞间通讯功能(GJIC)的调节作用。方法在培养的人脐静脉内皮细胞(HUVEC),预先加入不同浓度的Rut(10、03、01 μmol·L-1),处理10 min后加入LPC(10 mg·L-1)共同孵育24 h。应用辣椒素受体(TRPV1)竞争性拮抗剂CAPZ(10 μmol·L-1)研究TRPV1是否介导Rut的保护效应。荧光定量PCR检测缝隙连接蛋白Cx37、Cx40的mRNA水平,Western blot检测Cx37和Cx40的蛋白水平,划痕负载试验测定GJIC功能。检测内皮细胞活力(MTT法)、活性氧(ROS)水平和细胞培养液中NO水平及单核细胞黏附,以评价内皮细胞损伤程度。结果LPC明显下调HUVEC中Cx37和Cx40的mRNA和蛋白水平,抑制缝隙连接染料迁移。而Rut可明显恢复Cx37、Cx40的表达,改善GJIC功能,并减轻LPC诱导血管内皮损伤,表现为增加细胞活力和NO水平,抑制ROS生成和单核细胞黏附。预先给予CAPZ可取消这些效应。结论Rut可抑制LPC诱导的内皮细胞损伤,恢复Cx37和Cx40的表达而改善GJIC,其机制与激活TRPV1有关。
Abstract:
AimTo investigate the protective effect of rutaecarpine against endothelial cell damage and the dysfuction of gap junction induced by lysopbosphatidylcholine(LPC). MethodsCell damage was induced by treatment with LPC(10 mg·L-1)for 24 h. HUVECs were pretreated with rutaecarpine(10,03,01 μmol·L-1) 10 min before LPC treatment. To explore whether the protective effects of rutaecarpine on HUVECs involved TRPV1, the TRPV1 competitive antagonist CAPZ(10 μmol·L-1)was used. The level of Cx37 and Cx40 mRNA was detected by RealTime PCR, protein level was measured by Western blot, and the function of gap junction intercellular communication(GJIC) was determined by intercellular transfer of Lucifer yellow.The endothelial cell injury was evaluated by cell viability (MTT), production of ROS, the level of NO in the medium, and the adhesion ratio of monocyteendothelialcell. ResultsExposure of HUVECs to LPC significantly decreased the expression of Cx37 and Cx40, both the level of mRNA and protein, and inhibited intercellular transfer of Lucifer yellow. Pretreatment with rutaecarpine significantly upregulated the expression of Cx37 and Cx40, and improved the function of GJIC. Rutaecarpine attenuated the endothelial cell injury, which included increasing the cell viability and NO production, decreasing the production of ROS, and inhibiting the monocyte adhesion. Pretreatment with CAPZ abolished the protective effect of rutaecarpine on gap junction and endothelial cells. ConclusionRutaecarpine prevents the endothelial cell injury induced by LPC, and improves the function of GJIC by increasing the expression of Cx37 and Cx40. These effects are related to the activation of TRPV1.

参考文献/References:

[1]Brisset A C, Isakson B E, Kwak B R. Connexins in vascular physiology and pathology[J]. Antioxid Redox Signal, 2009,11(2):267-82.
[2]Pfenniger A, Chanson M, Kwak B R. Connexins in atherosclerosis[J]. Biochim Biophys Acta, 2013,1828(1):157-66.
[3]陈敏,蒋丽萍,洪涛. 缝隙连接蛋白在动脉粥样硬化形成和防治中的作用[J]. 中国药理学通报,2010,26(10):1271-4.
[3]Chen M, Jiang L P, Hong T. The role of gap junction connexin in the formation,precaution and treatment of atherosclerosis[J]. Chin Pharmacol Bull,2010,26(10):1271-4.
[4]Wong C W, Christen T, Roth I, et al. Connexin37 protects against atherosclerosis by regulating monocyte adhesion[J]. Nat Med, 2006,12(8):950-4.
[5]Chadjichristos C E, Scheckenbach K E, van Veen T A, et al. Endothelialspecific deletion of connexin40 promotes atherosclerosis by increasing CD73dependent leukocyte adhesion[J]. Circulat,2010,121(1):123-31.
[6]Pfenniger A, Wong C, Kwak B R, et al. Shear stress modulates the expression of the atheroprotective protein Cx37 in endothelial cells[J]. J Mol Cell Cardiol,2012,53(2):299-309.
[7]胡长平,李元建. 吴茱萸碱和吴茱萸次碱的药理学研究进展[J]. 中国药理学通报,2003,19(10):1084-7.
[7]Hu C P, Li Y J. Research progress in pharmacological actions of evodiamine and rutaecarpine[J]. Chin Pharmacol Bull,2003,19(10):1084-7.
[8]Peng J, Li YJ. The vanilloid receptor TRPV1: role in cardiovascular and gastrointestinal protection [J]. Eur J Pharmacol, 2010,627(1-3):1-7.
[9]Luo D, Peng W J, Li Y J, et al. Transient receptor potential vanilloid 1mediated expression and secretion of endothelial cellderived calcitonin generelated peptide[J]. Regul Pept, 2008,150(1-3):66-72.
[10]Heo S K, Yun H J, Yi H S, et al. Evodiamine and rutaecarpine inhibit migration by LIGHT via suppression of NADPH oxidase activation[J]. J Cell Biochem, 2009,107(1):123-33.
[11]邱模昌,余艳荣,罗丹,等. 吴茱萸次碱对内皮细胞损伤的保护效应及机制 [J]. 时珍国医国药,2013,24(3):580-2.
[11]Qiu M C, Yu Y R, Luo D, et al. Protective effect of rutaecarpine(RUT) on human umbilical vein endothelial cell(HUVEC) injury[J]. Lishizhen Med Mat Med Res,2013,24(3):580-2.
[12]Morel S, Burnier L, Kwak B R. Connexins participate in the initiation and progression of atherosclerosis[J]. Semin Immunopathol,2009,31(1):49-61.
[13]Kwak B R, Mulhaupt F, Veillard N, et al. Altered pattern of vascular connexin expression in atherosclerotic plaques[J]. Arterioscler Thromb Vasc Biol, 2002,22(2):225-30.
[14]LooftWilson R C, Billaud M, Johnstone S R, et al. Interaction between nitric oxide signaling and gap junctions: Effects on vascular function[J]. Biochim Biophys Acta, 2012,1818(8):1895-902.
[15]Schilling T, Eder C. Importance of the nonselective cation channel TRPV1 for microglial reactive oxygen species generation[J]. J Neuroimmunol,2009,216(1-2):118-21.

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备注/Memo

备注/Memo:
收稿日期:2013-07-28,
修回日期:2013-08-27
基金项目:国家自然科学基金资助项目(No 30801399);江西省自然科学基金资助项目(No 2010GQY0240);江西省教育厅科学技术研究项目(No GJJ10312)
作者简介:刘勇(1988-),男,硕士生,研究方向:心血管药理学,Email:871964552@qq.com; 余艳荣(1988-),女,硕士生,研究方向:心血管药理学,共同第一作者; 罗丹(1979-),女,博士,副教授,研究方向:心血管药理学,通讯作者,Email:thinker_20080502@126.com
更新日期/Last Update: 2013-11-11