[1]倪伟建,丁海华,唐丽琴,等.小檗碱对糖尿病肾病大鼠肾组织VEGF表达的影响[J].中国药理学通报,2015,(06):795-800.
 NI Wei-jian,DING Hai-hua,TANG Li-qin,et al.Effect of berberine on expression of vascular endothelial growth factor in diabetic nephropathy rats[J].Chinese Pharmacological Bulletin,2015,(06):795-800.
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小檗碱对糖尿病肾病大鼠肾组织VEGF表达的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2015年06期
页码:
795-800
栏目:
论 著
出版日期:
2015-06-15

文章信息/Info

Title:
Effect of berberine on expression of vascular endothelial growth factor in diabetic nephropathy rats
作者:
倪伟建1丁海华1唐丽琴12魏 伟1
1.安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室, 安徽抗炎免疫 药物协同创新中心, 安徽 合肥 230032; 2.安徽医科大学附属省立医院,安徽 合肥 230001
Author(s):
NI Wei-jian1 DING Hai-hua1 TANG Li-qin12 WEI Wei1
1. Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Antiinflammatory and Immune Medicine, Anhui Medical University; Ministry of Education,Hefei 230032, China; 2. Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China
关键词:
小檗碱 糖尿病肾病 血管内皮生长因子 肾小球 足细胞 肾脏保护作用
Keywords:
berberine diabetic nephropathy vascular endothelial growth factor glomerulus podocyte renoprotective effect
分类号:
R-332; R284.1; R322.61; R587.2; R977.6
文献标志码:
A
摘要:
目的 观察小檗碱(berberine, BBR)对糖尿病肾病(diabetic nephropathy, DN)大鼠肾脏的保护作用。方法 采用高糖高脂饲料联合低剂量(35 mg·kg-1)链脲佐菌素(streptozotocin, STZ)诱导糖尿病肾病大鼠模型。随机分为空白对照组、模型组、BBR(50 mg·kg-1)、BBR(100 mg·kg-1)和BBR(200 mg·kg-1)剂量治疗组结果。分别在给药2、4、6、8周时检测大鼠空腹血糖变化,给药8周后测定大鼠血肌酐和尿素氮含量,尿总蛋白含量的变化; PAS染色观察DN肾脏组织病理学改变; 免疫组织化学法检测肾脏组织血管内皮生长因子表达; Western blot法检测DN肾脏组织血管内皮生长因子表达水平。结果 与正常对照组相比,模型组血糖及尿总蛋白指标明显升高,产生明显的形态学异常变化,同时血管内皮生长因子的表达量明显升高; 与模型组相比,小檗碱给药组大鼠血糖和尿总蛋白量均明显下降; 同时血液指标血肌酐和尿素氮水平也不同程度的下降; 另外,BBR给药组还可明显改善糖尿病肾病大鼠肾脏病理学异常,降低肾脏血管内皮生长因子的表达。 结论 小檗碱可明显改善糖尿病肾病大鼠发病过程中的生化指标和肾脏病理损伤,这可能与其影响糖尿病肾病状态下肾脏组织中血管内皮生长因子的表达有关。
Abstract:
Aim To investigate the renoprotective effect of berberine in diabetic nephropathy rat model. Methods The rat model of DN was induced by intraperitoneal injection of streptozotocin(STZ)after fed with high sugar and high fat diet for six weeks. The rats were divided into 5 groups randomly, i.e. normal control group, model group, BBR(50 mg·kg-1), BBR(100 mg·kg-1)and BBR(200mg·kg-1)treatment group. The fasting blood glucose(FBG)was evaluated at 2, 4, 6,8 week respectively. The pathological changes in the kidney were determined by PAS staining. The expression of vascular endothelial growth factor(VEGF)was detected by immunohistochemistry and Western blot respectively. Results Compared with normal control group, the value of FBG, SCr, BUN and UTP of model group were sharply increased. Compared with model group, the value of FBG in berberine different dosage treatment groups were significantly decreased to various degrees, and berberine different dosage treatment could decrease the levels of SCr, BUN and UTP in different degree. Berberine could surpress the alterations of pathological changes in the kidneys and downregulate the expression levels of VEGF in the kidney of diabetic rats with nephropathy. Conclusion Berberine could significantly ameliorate the biochemical indicators and renal injury of the model rats through affecting the abnormal expression levels of VEGF in the kidney.

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备注/Memo

备注/Memo:
收稿日期:2015-01-17,修回日期:2015-02-27 基金项目:国家自然科学基金资助项目(No 81102864,81073109); 安徽省中医药科研课题(No 2012zy48) 作者简介:倪伟建(1990-), 男, 硕士生,研究方向:内分泌及代谢药理学, E-mail:jayni1990@126.com; 唐丽琴(1972-), 女, 博士,教授, 主任药师, 硕士生导师,研究方向:内分泌及代谢药理学, 通讯作者, E-mail:tulcyl@vip.sina.com; 魏 伟(1960-), 男, 博士, 教授, 博士生导师, 研究方向:抗炎免疫药理学, 通讯作者, E-mail:wwei@ahmu.edu.cn
更新日期/Last Update: 1900-01-01