[1]王艳明,刘 瑛,阎新燕,等.甘草查尔酮A抑制小鼠黑色素瘤B16F10细胞增殖机制研究[J].中国药理学通报,2015,(07):967-972.[doi:10.3969/j.issn.1001-1978.2015.07.016]
 WANG Yan-ming,LIU Ying,YAN Xin-yan,et al.Inhibitory effects of Licochalcone A on proliferation of melanoma B16F10 cells[J].Chinese Pharmacological Bulletin,2015,(07):967-972.[doi:10.3969/j.issn.1001-1978.2015.07.016]
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甘草查尔酮A抑制小鼠黑色素瘤B16F10细胞增殖机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2015年07期
页码:
967-972
栏目:
论 著
出版日期:
2015-07-15

文章信息/Info

Title:
Inhibitory effects of Licochalcone A on proliferation of melanoma B16F10 cells
作者:
王艳明1刘 瑛1阎新燕1司玲玲1高彩霞2于丽娜2郑秋生13
1. 石河子大学药学院,新疆特种植物药资源教育部重点实验室,新疆 石河子 832002; 2. 滨州医学院中西医结合学院, 山东 烟台 264003; 3. 烟台大学生命科学学院,山东 烟台 264005
Author(s):
WANG Yan-ming1LIU Ying1 YAN Xin-yan1 SI Ling-ling1 GAO Cai-xia2 YU Li-na2 ZHENG Qiu-sheng13
1.Pharmacy School, Shihezi University, Key Laboratory of Xinjiang Endemic Phytomedicine Resources of Ministry of Education, Shihezi Xinjiang 832002, China; 2.Binzhou Medical University, Yantai Shandong 264003, China; 3.College of Life Science, Yantai University, Yantai Shandong 264005, China
关键词:
B16F10 甘草查尔酮A 增殖抑制率 黑色素含量 细胞分化 细胞凋亡 细胞周期阻滞
Keywords:
B16F10 Licochalcone A the rate of proliferation melanin level differentiation apoptosis cell cycle arrest
分类号:
R-332; R284.1; R329.24; R329.28; R739.5
DOI:
10.3969/j.issn.1001-1978.2015.07.016
文献标志码:
A
摘要:
目的 研究甘草查尔酮A抑制B16F10细胞增殖机制。方法 SRB法检测甘草查尔酮A对B16F10细胞增殖影响,Giemsa染色法观察细胞形态变化,比色法检测B16F10细胞内、外黑色素含量,Annexin V-FITC/PI 双染检测细胞凋亡率,流式细胞术测定细胞周期分布,Q-PCR法检测细胞凋亡相关基因B淋巴细胞-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、细胞周期蛋白(Cyclin E2)和细胞周期蛋白依赖性激酶-2(CDK2)的mRNA表达。结果 甘草查尔酮A能有效抑制B16F10细胞增殖,呈现浓度依赖性和时间依赖性; 随药物浓度增加,细胞增殖速度降低,细胞形态由树突状变为固缩圆球状,并伴有黑色素颗粒物出现,且细胞内、外黑色素含量呈浓度依赖性增加趋势,甘草查尔酮A能使细胞阻滞在G1期,在低浓度时,诱导细胞分化,高浓度时,诱导细胞凋亡; 同时,甘草查尔酮A下调凋亡相关蛋白Bcl-2/Bax比率,抑制周期相关蛋白Cyclin E2、CDK2的mRNA表达。结论 甘草查尔酮A抑制B16F10细胞增殖机制可能是通过使B16F10细胞G1期阻滞,进而诱导细胞分化和凋亡。
Abstract:
Aim To investigate the mechanism of the melanoma B16F10 cells proliferation induced by Licochalcone A in vitro. Methods The proliferation of B16F10 cells induced by Licochalcone A was determined by SRB method. The morphological changes were observed using Giemsa staining under the phase contrast microscope equipped with a digital camera. The melanin level was assessed by colorimetric method. The apoptotic rate was determined by Annexin V-FITC/PI assay. Cell cycle distribution was determined by flow cytometry. The mRNA expression levels of B cell lymphoma/lewkmia-2(Bcl-2), Bcl-2 associated X protein(Bax), the cell cycle protein CyclinE2 and cyclin-dependent kinase-2(CDK2)CDK2 were detected using Q-PCR analysis. Results The proliferation of B16F10 cells treated with Licochalcone A was effectively inhibited in a concentration and time-dependent manner. A clear morphological change was observed with the increasing concentration of Licochalcone A in B16F10 cells, the dendrite-like projections changed to the narrowing ball shape, which was associated with the increasing melanin level. The low concentration of Licochalcone A could induce B16F10 differentiation, and the high concentration of Licochalcone A could induce B16F10 apoptosis, which was accompanied with the increasing G1 phase in cell cycle. The mRNA expression levels of Bcl-2 /Bax, CyclinE2 and CDK2 were markedly reduced. Conclusion Licochalcone A can effectively inhibit the proliferation of B16F10 cells, induced cell cycle arrest at G1 phase, and further induced differentiation and apoptosis.

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备注/Memo

备注/Memo:
收稿日期:2015-03-09,修回日期:2015-04-28 基金项目:国家自然科学基金资助项目(No 31471338,81260338); 兵团重点领域创新团队建设计划项目,石河子科技计划课题(No 2014QY16) 作者简介:王艳明(1987-),女,硕士,研究方向:肿瘤药理学,E-mail: 1040527954@qq.com; 郑秋生(1967-),男,博士,博士生导师,教授,研究方向:肿瘤药理学,E-mail: zqsyt@sohu.com
更新日期/Last Update: 1900-01-01