[1]王颖婉,李叶丽,王俊逸,等.PPARα、PPARγ的上调参与淫羊藿苷对自发性高血压大鼠心室重构的调节[J].中国药理学通报,2015,(08):1117-1120.[doi:10.3969/j.issn.1001-1978.2015.08.018]
 WANG Ying-wan,LI Ye-li,WANG Jun-yi,et al.Icariin attenuates left ventricular remodeling in SHR by up-regulating PPARα and PPARγ[J].Chinese Pharmacological Bulletin,2015,(08):1117-1120.[doi:10.3969/j.issn.1001-1978.2015.08.018]
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PPARα、PPARγ的上调参与淫羊藿苷对自发性高血压大鼠心室重构的调节()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2015年08期
页码:
1117-1120
栏目:
论 著
出版日期:
2015-08-15

文章信息/Info

Title:
Icariin attenuates left ventricular remodeling in SHR by up-regulating PPARα and PPARγ
作者:
王颖婉李叶丽王俊逸华 亮杨丹莉
遵义医学院药理学教研室,基础药理省部共建教育部重点实验室,贵州 遵义 563000
Author(s):
WANG Ying-wan LI Ye-li WANG Jun-yi HUA Liang YANG Dan-li
Key Laboratory for Basic Pharmacology of Ministry of Education, Dept of Pharmacology, Zunyi Medical University, Zunyi Guizhou 563000,China
关键词:
淫羊藿苷 左心室重构 过氧化物酶体增殖物激活受体α 过氧化物酶体增殖物激活受体γ 自发性高血压大鼠 蛋白印迹技术
Keywords:
icariin left ventriular remodeling PPARα PPARγ SHR Western blot
分类号:
R-332; R322.11; R331.31; R329.11; R544.1
DOI:
10.3969/j.issn.1001-1978.2015.08.018
文献标志码:
A
摘要:
目的 观察淫羊藿苷(icariin,Ica)对自发性高血压大鼠(SHR)心室重构的作用,并探讨其机制。方法 14周龄♂ SHR大鼠21只随机分为模型组,Ica低、高剂量组(20、40 mg·kg-1,ig, bid),14周龄♂ WKY大鼠为空白对照组,空白对照组和模型组给予等体积的双蒸水灌胃。适应性喂养1周,连续灌胃12周后处死全部动物,采用双抗体夹心法测定左心羟脯氨酸的含量; Masson染色观察左心室组织病理学变化; real-time RT-PCR、Western blot分别检测PPARα、PPARγ mRNA和蛋白的表达。结果 与空白对照组相比,模型组出现心室重构,心肌纤维化,心肌组织羟脯氨酸含量明显上升(P<0.01),PPARα、PPARγ mRNA和蛋白表达明显降低(P<0.05或P<0.01)。与模型组相比,Ica低、高剂量组心肌细胞排列较整齐,心肌纤维化减少,且心肌羟脯氨酸含量降低,PPARα、PPARγ mRNA和蛋白表达升高(P<0.05 或P<0.01)。结论 淫羊藿苷具有减轻自发性高血压大鼠心室重构的作用,其机制可能与上调PPARα、PPARγ 有关。
Abstract:
Aim To investigate the effect of Icariin(Ica)on remodeling of left ventricular in SHR and explore the mechanism. Methods Twenty-one male SHR aged 14 weekswere randomly divided into model group(n=7), low-dose of Ica-treated group(20 mg·kg-1.bid,n=7),high-dose of Ica-treated group(40 mg·kg-1.bid,n=7), and WKY as control group(n=7). Low- and high-dose of Ica-treated groups were given Ica from the age of 14 weeks to 26 weeks. The other rats in the model group and control group were given the same amount of double distilled water. Then, the content of hydroxyproline(Hyp)was measured by ELISA. The morphological changes of the left ventricular were observed by Masson staining. The mRNA and the protein expression of PPARα and PPARγ were examined by real time RT-PCR and Western blot technique respectively. Results Compared with the normal control group, interstitium fibrosis and myofibrilla were lined up in disorder; the content of Hyp was increased and the mRNA and protein expression of PPARα and PPARγ were down-regulated in model group(P<0.01). Compared with the model group,the myocardial cells were arranged less disorderly and the myocardial fibrosis was reduced; the content of Hyp was decreased in low-and high-dose of Ica-treated groups(P<0.01 or P<0.05); the mRNA and protein expression of PPARα and PPARγ were up-regulated in low- and high-dose of Ica-treated groups(P<0.01 or P<0.05). Conclusion Ica may attenuate left ventricular remodeling in SHR by up-regulating PPARα and PPARγ.

参考文献/References:

[1] Kolesnyk IuM, Kolesnyk MIu, Abramov AV. Pathological remodeling of myocardium in spontaneous hypertensive rats with experimental diabetes mellitus: the role of mitochondrial dysfunction [J].Fiziol Zh,2014,60(3):18-26.
[2] Karaahmet T,Tigen K,Dundar C,et al. The effect of cardiac fibrosis on left ventricular remodeling,diastolic function,and N-terminal pro-B-type natriuretic peptide levels in patients with nonischemic dilated cardiomyopathy [J]. Echocardiography, 2010,27(8):854-960.
[3] 杨永曜,李隆贵. PPARα,PPARγ及其配体对心室重构作用的研究进展[J]. 临床心血管病杂志,2006,22(1):61-3.
[3] Yang Y Y, Li L G. Progress in research on the effect of left ventricular remodeling by PPARα,PPARγ and and its ligand[J]. J Clin Cardiol(China), 2006,22(1):61-3.
[4] 李叶丽,王颖婉,李意奇,等. 淫羊藿苷通过降低醛固酮水平抗自发性高血压大鼠肾间质纤维化[J]. 中国药理学通报,2014,30(4):519-22.
[4] Li Y L, Wang Y W, Li Y Q,et al. Anti-renal interstitial fibrosis effect of icariin in SHR by reducing aldosterone levels[J].Chin Pharmacol Bull, 2014,30(4):519-22.
[5] 张丽梅,杨 竞,李意奇,等.淫羊藿苷抑制TGF-β1 /Smad2 信号通路改善压力超负荷所致的大鼠心肌纤维化[J]. 中国药理学通报, 2013,29(10):1422-5.
[5] Zhang L M,Yang J,Li Y Q,et al. Anti-myocardial fibrosis activity of icariin in pressure overload rats through inhibition of TGF-β1 /Smad2 signal pathway[J]. Chin Pharmacol Bull, 2013,29(10):1422-5.
[6] Bradshaw A D, Baicu C F, Rentz T J, et al. Pressure overload-induced alterations in fibrillar collagen content and myocardial diastolic function: role of secreted protein acidic and rich in cysteine(SPARC)in post-synthetic procollagen processing[J]. Circulation, 2009, 119(2): 269-80.
[7] 于学军,何作云,戚文航. 自发性高血压大鼠左室不同部位心肌纤维化的动态变化[J]. 临床心血管杂志,2001,17(10):466-8.
[7] Yu X J,He Z Y,Qi W H. Study of dynamic changes of myocardial fibrosis in different areas of SHRs' left ventricle[J]. J Clin Cardiol(China), 2001,17(10):466-8.
[8] Usuda D, Kanda T. Peroxisome proliferator-activated receptors for hypertension [J]. World J Cardiol, 2014,26(8):744-54.
[9] Hafstad A D, Khalid A M, Hagve M, et al. Cardiac peroxisome proliferator-activated receptor-alpha activation causes increased fatty acid oxidation, reducing efficiency and post-ischaemic functional loss[J]. Cardiovasc Res, 2009, 83(3):519-26.
[10] Kim T, Yang Q. Peroxisome-proliferator-activated receptors regulate redox signaling in the cardiovascular system [J]. World J Cardiol, 2013,5(6):164-74.
[11] 张瑞英,莫成利,富 路. PPARα对动脉粥样硬化、急性心肌梗死﹑糖尿病心肌病的作用及机制研究进展[J]. 心血管病学进展, 2008,29(4):613-6.
[11] Zhang R Y, Mo C L,Fu L. Progress of mechanism and effect of PPARα on atherosclerosis acute myocardial infarction and diabetic cardiomyopathy[J]. Adv Cardiovasc Dis, 2008,29(4):613-6.
[12] Nanayakkara G, Viswaprakash N, Zhong J, et al. PPARγ activation improves the molecular and functional components of I(to)remodeling by angiotensin II[J]. Curr Pharm Des, 2013,19(27):4839-47.
[13] 曾正英,廖高鸿,吕雅斐,陈国良. PPARs激动剂在心脑血管疾病中的研究进展[J]. 中国药物化学杂志, 2014,24(2):147-56.
[13] Zheng Z Y, Liao G H, Lü Y F, Chen G L. Research progress of PPARs agonists on cardiovascularand cerebrovascular diseases [J]. Chin J Med Chem, 2014,24(2):147-56.

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备注/Memo

备注/Memo:
收稿日期:2015-03-14,修回日期:2015-04-15 基金项目:国家自然科学基金资助项目(No 81241142) 作者简介:王颖婉(1988-),女,硕士,研究方向:心血管药理学、抗炎免疫药理学,E-mail:763595559@qq.com; 杨丹莉(1971-),女,博士,教授,硕士生导师,研究方向:心血管药理学、抗炎免疫药理学,通讯作者,Tel:0852-8609623,E-mail:393707603@qq.com
更新日期/Last Update: 1900-01-01