[1]任 荔,陶伟伟,薛文达,等.越鞠丸石油醚部位潜在的快速抗抑郁作用与BDNF、TrkB蛋白表达的上调相关[J].中国药理学通报,2015,(12):1754-1759.[doi:10.3969/j.issn.1001.1978.2015.12.025]
 REN Li,TAO Wei-wei,et al.Potent rapid antidepressant effects of petroleum ether fraction of Yueju pill associated with BDNF and TrkB up-regulation[J].Chinese Pharmacological Bulletin,2015,(12):1754-1759.[doi:10.3969/j.issn.1001.1978.2015.12.025]
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越鞠丸石油醚部位潜在的快速抗抑郁作用与BDNF、TrkB蛋白表达的上调相关()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2015年12期
页码:
1754-1759
栏目:
复方药物药理学
出版日期:
2015-12-30

文章信息/Info

Title:
Potent rapid antidepressant effects of petroleum ether fraction of Yueju pill associated with BDNF and TrkB up-regulation
作者:
任 荔12陶伟伟12薛文达12吴颢昕12陈 刚12
南京中医药大学1.基础医学院转化系统生物学与神经科学研究中心、2.中医脑病重点实验室,江苏 南京 210023
Author(s):
REN Li1 2 TAO Wei-wei1 2 XUE Wen-da1 2 WU Hao-xin1 2 CHEN Gang1 2
1.Center for Translational Systems and Neuroscience, School of Basic Biomedical Science; 2. Key Laboratory of Integrative Biomedicine of Brain Diseases, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China
关键词:
越鞠丸 石油醚部位 快速抗抑郁 BDNF TrkB 氯胺酮
Keywords:
Yueju pill petroleum ether fraction rapid antidepressant BDNF TrkB ketamine
分类号:
R-332; R284.1; R322.81; R749.42; R971.43; R977.6
DOI:
10.3969/j.issn.1001.1978.2015.12.025
文献标志码:
A
摘要:
目的 研究越鞠丸石油醚部位是否具有快速抗抑郁潜力,并对其有效剂量及潜在的生物学机制进行了探讨。方法 将44只昆明种♂小鼠随机分成空白组(生理盐水组)、越鞠丸石油醚部位(YJ-PE)高、中、低剂量组及氯胺酮组。单次给药后30 min对昆明小鼠进行小鼠悬尾行为绝望测试。再将24只昆明种♂小鼠分成空白组、筛选出有效的越鞠丸石油醚部位低剂量组和氯胺酮组,单次给药24 h后进行小鼠悬尾行为绝望测试。利用Western blot实验分别检测给药后30 min及24 h小鼠海马区BDNF和TrkB蛋白的表达变化。结果 单次给药越鞠丸石油醚部位从30 min到24 h均能对其小鼠悬尾的行为绝望行为产生抗抑郁作用。给予越鞠丸石油醚部位30 min后小鼠海马中的BDNF及其受体TrkB的表达明显上调,且这种抗抑郁的作用机制可持续到24 h。结论 越鞠丸石油醚部位(低剂量)具有快速抗抑郁的药物潜力,且这种潜在的快速抗抑郁作用与BDNF及其受体TrkB的激活相关。
Abstract:
Aim To identify whether the petroleum ether fraction of Yueju pill(YJ-PE)could exhibit a potent rapid antidepressant effect and to investigate the underlying biological mechanism involved. Methods Fourty-four male KM mice were randomly assigned to five groups: the control group(saline group), low dosage of YJ-PE, middle dosage of YJ-PE, high dosage of YJ-PE and ketamine. After a single drug administration, we used tail suspension test(TST)to test the rapid antidepressant effect of YJ-PE in KM mice at 30 min. Another twenty-four male KM mice were assinged to three groups: control group, screened effective low dosage of YJ-PE group and ketamine group. We usd TST to assess these mice at 24 h post a single administration. Western blot was performed to examine the expression of BDNF and TrkB in hippocampus of KM mice at 30 min and 24 h post a single administration. Results An acute administration of YJ-PE decreased the immobility time of KM mice in TST and increased the expression of BDNF and TrkB as soon as 30min post a single administration and lasted at least for 24 hours. Conclusion YJ-PE(low dosage)exhibits a rapid antidepressant-like potent, and the potent rapid antidepressant effects of YJ-PE are associated with the up-regulation of BDNF and TrkB.

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更新日期/Last Update: 1900-01-01