[1]张明芳,齐元麟,王 丹,等.动脉平滑肌细胞STIM1过表达上调Akt/mTOR通路并促进细胞增殖[J].中国药理学通报,2016,(01):37-42.[doi:10.3969/j.issn.1001-1978.2016.01.009]
 ZHANG Ming-fang,QI Yuan-lin,WANG Dan,et al.STIM1 promotes arterial smooth muscle cells proliferation by regulating Akt/mTOR pathway[J].Chinese Pharmacological Bulletin,2016,(01):37-42.[doi:10.3969/j.issn.1001-1978.2016.01.009]
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动脉平滑肌细胞STIM1过表达上调Akt/mTOR通路并促进细胞增殖()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2016年01期
页码:
37-42
栏目:
论 著
出版日期:
2016-01-15

文章信息/Info

Title:
STIM1 promotes arterial smooth muscle cells proliferation by regulating Akt/mTOR pathway
文章编号:
1001-1978(2016)01-0037-06
作者:
张明芳1齐元麟2王 丹1王 情1陈富华1林默君1
福建医科大学基础医学院 1.生理学与病理生理学系、心血管科学研究室、2.生物化学与分子生物学系,福建 福州 350108
Author(s):
ZHANG Ming-fang1 QI Yuan-lin2 WANG Dan1 WANG Qing1 CHEN Fu-hua1 LIN Mo-jun1
1. Dept of Physiology and Pathophysiology, 2. Dept of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China
关键词:
肺动脉高压 动脉平滑肌细胞 基质相互作用分子1 细胞增殖 哺乳动物雷帕霉素靶蛋白 Akt信号通路
Keywords:
pulmonary hypertension arterial smooth muscle cells stromal interaction molecule 1(STIM1) cell proliferation mammalian target of rapamycin(mTOR) Akt signaling
分类号:
R322.121; R322.74; R322.35; R329.24; R544.022; R9
DOI:
10.3969/j.issn.1001-1978.2016.01.009
文献标志码:
A
摘要:
目的 观察基质相互作用分子1(stromal interaction molecule 1, STIM1)在肺动脉高压组织中的表达,并探讨STIM1对动脉平滑肌细胞增殖的影响及机制。 方法 一次性腹腔注射野百合碱(MCT)建立肺动脉高压大鼠模型; 实时定量PCR和蛋白印迹分别测定肺动脉高压大鼠肺组织STIM1 mRNA和蛋白的表达; 采用基因转染技术建立STIM1瞬时高表达的动脉平滑肌A7R5细胞模型; 用CCK-8法测定细胞增殖能力; 用蛋白印迹测定Akt/mTOR通路分子的表达。 结果 与对照组相比,MCT组的右心室收缩压(RVSP)和右心重量指数(RVMI)均明显增高(P<0.01),形态学观察可见肺小动脉平滑肌层明显增厚,管腔减小; MCT组大鼠STIM1 mRNA和蛋白表达量分别是对照大鼠的2.19和1.66倍; STIM1瞬时过表达的动脉平滑肌细胞与对照组相比,生长速度加快; STIM1过表达可明显增加Akt、mTOR、p70-S6K和4E-BP1的磷酸化水平,而对它们的蛋白水平没有影响。
Abstract:
Aim To investigate the expression of stromal interaction molecule 1(STIM1)in rat pulmonary arterial hypertension(PAH)tissues and effects of STIM1 on arterial muscle cells proliferation. Methods PAH was induced by a single intraperitoneal injection of MCT at a dose of 60 mg·kg-1. The mRNA or protein expressions of STIM1 in monocrotaline-induced pulmonary hypertensive rats were measured by real-time PCR or Western blot, respectively. The arterial smooth muscle cells A7R5 were transiently transfected with STIM1 plasmids to prepare STIM1 overexpressed cells. Cell proliferations were detected by using CCK-8 kits. The expressions of Akt/mTOR pathway molecules of A7R5 were measured by Western blot. Results The right ventricular systolic blood pressure(RVSP)and right ventricular mass index(RVMI)were markedly elevated in MCT-treated rats(P<0.01)in comparison to control rats. The mRNA and protein expression levels of STIM1 in monocrotaline-induced pulmonary hypertensive rats were 2.19 and 1.66 folds of control rats, respectively. STIM1 were transiently overexpressed in cultured A7R5. Cells transfected with STIM1 grew more quickly than non-transfected control. Overexpression of STIM1 significantly increased the phosphorylation of Akt, mTOR, p70-S6K, and 4E-BP1, but did not change their protein expression levels. Conclusion STIM1 are over-expressed in rat PAH tissues. Overexpression of STIM1 can promote arterial smooth muscle cells proliferation by regulating Akt/mTOR pathway.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(No 31171104); 福建省教育厅科技重点资助项目(No JA10130); 福建省自然科学基金资助项目(No 2015J01611, 2013J01373)
作者简介:张明芳(1973-),女,博士,副教授,研究方向:心血管病理生理学,E-mail:mfzhang@fjmu.edu.cn; 林默君(1964-),男,博士,教授,博士生导师,通讯作者,研究方向:循环生理学,Tel: 0591-22862006,E-mail:linmojunfz@163.com
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