[1]陈 瑶,余细勇.Hedgehog通路对心肌梗死及心肌细胞缺血缺氧的作用机制研究[J].中国药理学通报,2016,(05):676-680.[doi:10.3969/j.issn.1001-1978.2016.05.016]
 CHEN Yao,YU Xi-yong.Effect of Hedgehog pathway on myocardial infarction and hypoxic-ischemic cardiomyocytes[J].Chinese Pharmacological Bulletin,2016,(05):676-680.[doi:10.3969/j.issn.1001-1978.2016.05.016]
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Hedgehog通路对心肌梗死及心肌细胞缺血缺氧的作用机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2016年05期
页码:
676-680
栏目:
论 著
出版日期:
2016-05-15

文章信息/Info

Title:
Effect of Hedgehog pathway on myocardial infarction and hypoxic-ischemic cardiomyocytes
文章编号:
1001-1978(2016)05-0676-06
作者:
陈 瑶1余细勇12
1.广东省心血管病研究所、广东省人民医院,广东 广州 510080;
2.广州医科大学药学院, 广东 广州 511436
Author(s):
CHEN Yao1YU Xi-yong12
1.Guangdong Cardiovascular Institute, Guangdong General Hospital,Guangzhou 510080,China;
2.School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436,China
关键词:
Hedgehog 通路 心肌梗死 缺血 缺氧 H2O2 心肌细胞
Keywords:
Hedgehog pathway myocardial infarction ischemia hypoxia H2O2 myocardial cells
分类号:
R-332; R322.11; R329.24; R542.22; R845.22
DOI:
10.3969/j.issn.1001-1978.2016.05.016
文献标志码:
A
摘要:
目的 探讨Sonic Hedgehog信号通路在正常心脏和缺血缺氧的心肌细胞的激活与表达。方法 用左前降支结扎法构建心肌梗死模型,并用超声心动图鉴定。采用Western blot和免疫荧光染色分别检测12例心肌梗死组织,9例正常心肌组织,H9C2及H2O2诱导下的H9C2细胞的Shh、Ptch-1、Smo、Gli-1的表达。注射激动剂及抑制剂于H2O2诱导的缺血缺氧H9C2的细胞模型,再次检测Shh、Ptch-1、Smo、Gli-1的表达及强弱。结果 Shh、Gli-1在心肌梗死心脏表达,Ptch-1、Smo在正常心脏及心肌梗死心脏均表达,缺血缺氧H9C2细胞模型的Shh、Gli-1表达增加。H2O2诱导下的H9C2表达Gli-1、Shh,Ptch-1、Smo在正常H9C2和H2O2诱导下的H9C2均有表达。结论 Shh信号通路在缺血与氧化应激条件下被激活,并且具有促进心肌细胞的损伤修复作用。
Abstract:
Aim To investigate the effect of Sonic Hedgehog on normal hearts and hypoxic-ischemic myocardial cells.Methods A method for left anterior descending artery(LAD)ligation was employed to construct the myocardial infarction model, and ultrasonic cardiogram was used for identification. Western blot and immunofluorescence staining were used to detect expressions of Shh, Ptch-1, Smo and Gli-1 in H9C2 cells and H2O2-induced H9C2 cells, and that in 12 cases of myocardial infarction tissues and 9 cases of normal myocardium, respectively. Agonist and antagonist of Shh pathway were adminstered in the hypoxic-ischemic myocardial H2O2-induced H9C2 cell model, and once again expressions and strength of Shh, Ptch-1, Smo, Gli-1 were detected.Results Shh and Gli-1 were not expressed in normal hearts, but expressed in hearts with myocardial infarction; Ptch-1 and Smo were expressed in both normal hearts and hearts with myocardial infarction. Under the action of agonist, expressions of Shh and Gli-1 increased in the hypoxic-ischemic H9C2 cell model. Similarly, Shh and Gli-1 were not expressed in normal H9C2 cells, but in H2O2-induced H9C2 cells, and Ptch-1 and Smo were expressed in both normal H9C2 and in H2O2-induced H9C2 cells.Conclusion Shh signaling pathway can be activated in the condition of ischemia and oxidative stress, and then it promotes the repairing of myocardial cell damage.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(No 81120108003,8133 0007); 广东省科技计划重点项目(No 2014 A050503047, 2015B020225006)
作者简介:陈 瑶(1987-),女,硕士生,主治医师,研究方向: 心血管药理学,E-mail:1597384174@qq.com;
余细勇(1962-),男,博士,教授,博士生导师,研究方向:心血管药理学,通讯作者,E-mail:yuxycn@aliyun.com
更新日期/Last Update: 2015-05-15