[1]曹莉莎,张 芳,罗 文,等.抑郁大鼠海马组织的比较蛋白质组学研究[J].中国药理学通报,2016,(05):697-702.[doi:10.3969/j.issn.1001-1978.2016.05.020]
 CAO Li-sha,ZHANG Fang,LUO Wen,et al.Analysis of CUMS-induced differentially expressed proteins in rat hippocampus by ITRAQ[J].Chinese Pharmacological Bulletin,2016,(05):697-702.[doi:10.3969/j.issn.1001-1978.2016.05.020]
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抑郁大鼠海马组织的比较蛋白质组学研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2016年05期
页码:
697-702
栏目:
论 著
出版日期:
2016-05-15

文章信息/Info

Title:
Analysis of CUMS-induced differentially expressed proteins in rat hippocampus by ITRAQ
文章编号:
1001-1978(2016)05-0697-06
作者:
曹莉莎张 芳罗 文陈 静何淼泉王继生
绵阳市第三人民医院临床药学科,四川 绵阳 621000
Author(s):
CAO Li-sha ZHANG Fang LUO Wen CHEN Jing HE Miao-quan WANG Ji-sheng
Dept of Clinical Pharmacy, the Third Hospital of Mianyang, Mianyang Sichuan 621000,China
关键词:
ITRAQ 蛋白质组学 抑郁 海马组织 质谱 CUMS
Keywords:
ITRAQ proteomics depression hippocampus mass spectrum CUMS
分类号:
R-322; R322.11; R341; R749.42; R977.6
DOI:
10.3969/j.issn.1001-1978.2016.05.020
文献标志码:
A
摘要:
目的 比较慢性不可预见性温和应激(chronic unpredictable mild stress,CUMS)大鼠与健康大鼠的海马组织,筛选差异表达蛋白质。以期从蛋白质组学角度阐明其发病机制,并为寻找生物标志物提供靶标。方法 采用CUMS建立大鼠抑郁模型。运用8标ITRAQ技术,结合2D LC-MS/MS分析CUMS大鼠与健康大鼠的海马组织差异表达蛋白质。结果 共鉴定出5 109个蛋白,差异蛋白33个,其中8个蛋白表达上调,25个蛋白表达下调。结论 ITRAQ结合LC-MS/MS技术能快速有效地进行蛋白质组学研究,为我们研究抑郁症的发生、发展机制以及发现新的生物标志物提供了有力平台。
Abstract:
Aims To compare hippocampus between CUMS rats and normal rats, to find differentially expressed proteins and to explore the pathogenesis of depression in the protein levels and biological marker.Methods Chronic unpredicted mild stress was taken to establish rat depression model. The ITRAQ-labeled proteins and peptides were separated by the cation column, and differentially expressed proteins were detected and identified by 2D LC-MS/MS. The functions of proteins were analyzed by bioinformatics.Results Totally 5 109 proteins were identified, 33 differentially expressed proteins were identified, the expressions of 8 proteins were increased and 25 proteins downregulated. Conclusion ITRAQ based sereening is effective in discovering the nosogenesis of depression and new biological marker.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:四川省卫生厅科研课题(No 120320)
作者简介:曹莉莎(1987-),女,硕士,药师,研究方向:神经精神药理学及蛋白质组学,E-mail:285714739@qq.com;
王继生(1974-),男,博士,主任药师,硕士生导师,研究方向:神经精神药理学与药物蛋白质组学,通讯作者,E-mail:24475434@qq.com
更新日期/Last Update: 2015-05-15