[1]周海玉,戴 丽,王德凤,等.基于荧光定量PCR评测热性中药成分对TRPV1通道功能的影响[J].中国药理学通报,2016,(10):1395-1398.[doi:10.3969/j.issn.1001-1978.2016.10.013]
 ZHOU Hai-yu,DAI Li,WANG De-feng,et al.Evaluation of regulational function of ingredients from hot herbs on TRPV1 channel based on 7900 PCR instrument[J].Chinese Pharmacological Bulletin,2016,(10):1395-1398.[doi:10.3969/j.issn.1001-1978.2016.10.013]
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基于荧光定量PCR评测热性中药成分对TRPV1通道功能的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2016年10期
页码:
1395-1398
栏目:
论著
出版日期:
2016-10-15

文章信息/Info

Title:
Evaluation of regulational function of ingredients from hot herbs on TRPV1 channel based on 7900 PCR instrument
文章编号:
1001-1978(2016)10-1395-04
作者:
周海玉戴 丽王德凤戴逸飞周炜炜孟晶隋 峰霍海如
中国中医科学院中药研究所,北京 100700
Author(s):
ZHOU Hai-yuDAI LiWANG De-fengDAI Yi-feiZHOU Wei-weiMENG JingSUI FengHUO Hai-ru
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
关键词:
热性中药成分 背根神经节 TRPV1通道 7900 PCR仪 温觉感受功能 能量代谢
Keywords:
ingredients from hot-nature herbs dorsal root ganglion neurons TRPV1 channels 7900 PCR instrument thermo-sensational function energy metabolism
分类号:
R-332;R282.71;R285.1;R322.85;R348.1;R392.11
DOI:
10.3969/j.issn.1001-1978.2016.10.013
文献标志码:
A
摘要:
目的 深入研究热性中药的生物学功能和分子机制。方法 该研究选择了临床常用的热性中药的主要活性成分,基于新型7900PCR仪尝试搭建背根神经节(DRG)神经元TRPV1通道温觉感知功能的检测评价系统,并对热性中药成分的TRPV1通道功能的调节作用进行了研究。结果 TRPV1通道能够被温度升高所激活,且该激活过程可被TRPV1特异性阻断剂辣椒平抑制,所选大部分热性中药成分均具有上调TRPV1通道功能的作用。结论 这些结果提示基于PCR仪建立的TRPV1通道功能检测体系适于药物调节TRPV1通道功能的研究; 热性中药表征的热性属性可能与其所含的活性成分能够提高TRPV1通道感知温度的活性,从而增加机体的能量代谢有关。
Abstract:
Aim To further study the molecular mechanism of the herbs with hot nature on the regulational action on TRPV1 channel based on the 7900 Real-time PCR instrument. Methods 7900 PCR instrument was applied to detect the intracellular flurescence of TRPV1 channel in the dorsal root ganglions(DRG)neurons and the effect on the TRPV1's thermo-sensational functions of the selected 11 ingredients from hot herbs was explored. Results TRPV1 channels could be activated by gradually elevated temperature; the activation process could be blocked by the TRPV1 specific blocking agent capsaizepine. Most of the ingredients from hot-nature herbs had the potential to up-regualate TRPV1 channel function. Conclusions The established TRPV1 channel detection system based on PCR instrument is suitable for the analysis of regulational functions of drugs on the heat-activated TRPV1 channel; the functions of hot herbs may be related to the up-regualtional effects of its active ingredients on the TRPV1 channel which will further up-regulate energy metabolism.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(No 81274112,81373986,81473372); 国家自然科学基金青年项目(No 81403125,81403322); 北京市自然科学基金项目(No 7152106)
作者简介:周海玉(1992-),女,硕士生,研究方向:中药学,Tel:010-64041008, E-mail: zhouhy@163.com;
隋 峰(1967-),男,研究员, 博士后合作导师,研究方向:中药药性和药理学,通讯作者,Tel:010-64041008,E-mail:suifeng2136@126.com;
霍海如(1960-),女,研究员,博士生导师,研究方向:中药药理和药性研究,通讯作者,Tel:010-64041008,E-mail:hairuh@icmm.ac.com
更新日期/Last Update: 2016-10-15