[1]徐新伟,王照岩,王瑞鸽,等.Raptor通过上皮-间质转化促进乳腺癌的侵袭与转移[J].中国药理学通报,2017,(08):1091-1095.[doi:10.3969/j.issn.1001-1978.2017.08.011]
 XU Xin-wei,WANG Zhao-yan,WANG Rui-ge,et al.Raptor induces migration and invasion of breast cancer through epithelial-mesenchymal transition[J].Chinese Pharmacological Bulletin,2017,(08):1091-1095.[doi:10.3969/j.issn.1001-1978.2017.08.011]
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Raptor通过上皮-间质转化促进乳腺癌的侵袭与转移()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2017年08期
页码:
1091-1095
栏目:
论著
出版日期:
2017-07-20

文章信息/Info

Title:
Raptor induces migration and invasion of breast cancer through epithelial-mesenchymal transition
文章编号:
1001-1978(2017)08-1091-05
作者:
徐新伟1王照岩1王瑞鸽1李洪利2尹崇高3刘雨清1
潍坊医学院1.病理学教研室、2.医学研究实验中心、3.护理学院,山东 潍坊 261053
Author(s):
XU Xin-wei1WANG Zhao-yan1WANG Rui-ge1LI Hong-li2YIN Chong-gao3LIU Yu-qing1
1.Dept of Pathology, 2.Medicine Research Center,3.Colloge of Nursing,Weifang Medical University, Weifang Shandong 261053, China
关键词:
Raptor 上皮-间质转化 乳腺癌 侵袭 转移 核质分离
Keywords:
Raptor epithelial-mesenchymal transition breast cancer invasion metastasis nucleus mass separation
分类号:
R329.24;R341;R737.902.2;R977.6
DOI:
10.3969/j.issn.1001-1978.2017.08.011
文献标志码:
A
摘要:
目的 探讨Raptor在乳腺癌侵袭与转移过程中的作用。方法 采用Western blot检测乳腺癌细胞MCF-7和MDA-MB-231(MDA231)中Raptor蛋白的表达情况。应用小干扰RNA技术下调乳腺癌细胞MDA231中Raptor的表达量,同时将过表达质粒转入乳腺癌细胞MCF-7中,应用Western blot法检测转染后各组细胞中上皮性钙黏着蛋白(E-cadherin)和波形蛋白(Vimentin)的表达; 应用Transwell体外侵袭实验检测转染后各组细胞的侵袭能力; 应用细胞核质分离实验检测转染后各组细胞中Snail蛋白的转核情况。结果 MCF-7细胞中Raptor蛋白的表达量明显低于MDA231细胞中的表达量; 转染后,与对照组Scr/MDA231相比,siRaptor/MDA231组中Raptor蛋白的表达量明显降低,Vimentin蛋白表达量降低,而E-cadherin 蛋白表达量升高; 与对照组MCF-7/Con相比,MCF-7/Raptor组中Raptor蛋白的表达量明显升高,并伴有Vimentin蛋白表达量升高,而E-cadherin 蛋白表达量降低。siRaptor/MDA231细胞组中穿过Matrigel小室的细胞数量明显减少(P<0.05); MCF-7/Raptor细胞组中穿过Matrigel小室的细胞数量明显升高(P<0.05)。细胞核质分离实验结果显示,siRaptor/MDA231组中Snail蛋白表达量较Scr/MDA231组中明显降低; MCF-7/Raptor组中Snail蛋白表达量较MCF-7/Con组中明显升高。结论 Raptor能够促进乳腺癌EMT的发生,对乳腺癌的侵袭与转移有重要作用。
Abstract:
Aim To study the molecular mechanism of Raptor in the migration and invasion of breast cancer cells and provide the clinical theory basis for prevention of breast cancer invasion and metastasis.Methods Western blot was used to detect the expression of Raptor protein in MCF-7 cells and MDA231 cells.The siRNA plasmids were used to transfect MDA231 cells. At the same time, the plasmid pcDNA3.1-Raptor was transfected into MCF-7 breast cancer cells. And Western blot was used to analyze the protein expression level of E-cadherin and Vimentin.Transwell was used to test the ability of invasion. Nucleus mass separation experiment was used to test the expression of Snail.Results The expression of Raptor protein in MDA231 cells was higher than in the MCF-7 cell. When the control group of Scr/MDA231 cells compared with siRaptor/MDA231, Raptor protein expression was decreased obviously after plasmid transfection interference, accompanied by reduction of Vimentin protein expression and increase of E-cadherin protein expression. Compared with MCF-7/Con, Raptor protein expression significantly increased in MCF-7/Raptor, accompanied by increase of Vimentin protein expression and reduction of E-cadherin protein expression.The number of cells through the artificial basilemma Transwell in siRaptor/MDA231 cells was significantly reduced(P<0.05), and the number of cells through the Transwell chambers artificial basilemma was significantly increased in MCF-7/Raptor(P<0.05). Nucleus mass separation experiment results showed that the expression of Snail decreased obviously in the siRaptor/MDA231 than in the Scr/MDA231, however,the expression of Snail was obviously higher in the MCF-7/Raptor.Conclusions Raptor can promote the occurrence of EMT in breast cancer, thus it promotes invasion and metastasis of breast cancer.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年基金项目(No 81402389,81641111); 山东省自然科学基金资助项目(No ZR2014HL077,ZR 2015HL065); 山东省高等学校科技计划(No J12LK03,J13LK03) 作者简介:徐新伟(1991-),女,硕士,研究方向:肿瘤病理学,E-mail:18765693066@163.com; 刘雨清(1962-),女,硕士,教授,研究方向:肿瘤病理学,通讯作者,E-mail: yuqingliu89@hotmail.com; 尹崇高(1979-),男,硕士,副教授,研究方向:乳腺肿瘤,通讯作者,E-mail: ycglihongli@163.com
更新日期/Last Update: 2017-07-20