[1]李照雪,胡思远,王吉烨,等.基于促甲状腺功能的姜黄素延长低温冷冻小鼠生存时间的作用机制研究[J].中国药理学通报,2017,(09):1291-1297.[doi:10.3969/j.issn.1001-1978.2017.09.020]
 LI Zhao-xue,HU Si-yuan,WANG Ji-ye,et al.Effect of curcumin in prolonging survival time of cryopreserved mice via promoting thyroid function[J].Chinese Pharmacological Bulletin,2017,(09):1291-1297.[doi:10.3969/j.issn.1001-1978.2017.09.020]
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基于促甲状腺功能的姜黄素延长低温冷冻小鼠生存时间的作用机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2017年09期
页码:
1291-1297
栏目:
论著
出版日期:
2017-08-20

文章信息/Info

Title:
Effect of curcumin in prolonging survival time of cryopreserved mice via promoting thyroid function
文章编号:
1001-1978(2017)09-1291-07
作者:
李照雪13胡思远2王吉烨13陈泽慧13张闪闪13张 波13
1.石河子大学药学院,2.石河子大学医学院第一附属医院心胸外科,3.新疆特种植物药资源教育部重点实验室,新疆 石河子 832002
Author(s):
LI Zhao-xue13 HU Si-yuan2 WANG Ji-ye13 CHEN Ze-hui13 ZHANG Shan-shan13 ZHANG Bo13
1.School of Pharmaceutics; 2.Dept of Cardiothoracic Surgery, the First Affiliated Hospital of Shihezi University; 3.Key Lab of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, Shihezi University, Shihezi Xinjiang 832002,China
关键词:
姜黄素 冷冻损伤 甲状腺 生存时间 昆明小鼠 丙硫氧嘧啶 左旋甲状腺素钠
Keywords:
curcumin cold injury thyroid survival time Kunming mice propylthiouracil sodium levothyroxine
分类号:
R-332;R282.71;R322.51;R347.2;R645;R977.14
DOI:
10.3969/j.issn.1001-1978.2017.09.020
摘要:
目的 低温冷冻会对恒温动物生理系统造成严重损害,寻找冻害防护的药物成为研究热点。方法 该文以姜黄素(curcumin,Cur)对小鼠冷冻损害保护作用与甲状腺功能评价为研究内容。定量分析小鼠(-20±1)℃冷冻暴露生存时间、代谢指标的变化; ELISA试剂盒检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)含量; HE染色观察甲状腺组织形态; 实时荧光定量PCR法检测甲状腺功能相关基因的表达。结果 姜黄素(12.5~50 mg·kg-1)腹腔注射给药能明显延长冷冻暴露小鼠的生存时间; 甲状腺HE形态学显示姜黄素逆转了冷冻导致的滤泡破损; 姜黄素组代谢指标改善明显,血清FT3、FT4激素含量明显增高,甲状腺功能相关基因甲状腺细胞钠碘转运体(sodium iodide symporter,Nis)、甲状腺球蛋白(thyroglobulin,Tg)、甲状腺过氧化物酶(thyroid peroxidase,Tpo)mRNA的表达明显上调,促甲状腺激素受体(thyroid-stimulating hormone receptor,Tshr)mRNA的表达下调,这些促甲状腺作用与左旋甲状腺素钠(sodium levothyroxine,L-T4)呈协同作用,且可被丙硫氧嘧啶(propylthiouracil,PTU)所抑制。结论 姜黄素延长小鼠生存的低温冷冻保护作用与其促进甲状腺功能密切相关。
Abstract:
Aim To look for cold-protective drugs treating cryogenic freezing that may bring great damage to animal physiological system.Methods The protective effect of curcumin on frozen damage and the changes of thyroid function in mice were studied in this study. Quantitative analysis of the changes in survival time and metabolic indexes in mice disposed at(-20±1)℃ was conducted. Mouse serum free triiodothyronine(FT3)and free thyroxine(FT4)contents were detected by ELISA kit. HE staining was used to observe thyroid tissue morphological items. The expression of genes related to thyroid function was assessed via real-time quantitative PCR.Results The intraperitoneal injection of curcumin(12.5~50 mg·kg-1)could remarkably prolong the survival time of mice when exposed to cryogenic freezing. HE staining results displayed a recovered thyroid injury in morphology in the curcumin group, further with a notably improved metabolic indexes and evidently increase in serum FT3 and FT4 levels. The real-time quantitative PCR results indicated that the expressions of sodium iodide symporter(Nis), thyroglobulin(Tg)and thyroid peroxidase(Tpo)were up-regulated, and the expression of thyroid-stimulating hormone receptor(Tshr)was down-regulated. Sodium levothyroxine collabrated with the promoting thyroid effects of curcumin, while propylthiouracil inhibited the effects.Conclusion Curcumin can prolong the survival time of the cryogenic freezing mice, which is closely related to its ability to promote thyroid function.

参考文献/References:

[1] Cheshire W P Jr. Thermoregulatory disorders and illness related to heat and cold stress[J].Auton Neurosci, 2016, 196:91-104.
[2] Pan Y, Wang Y, Zhao Y, et al. Inhibition of JNK phosphorylation by a novel curcumin analog prevents high glucose-induced inflammation and apoptosis in cardiomyocytes and the development of diabetic cardiomyopathy[J]. Diabetes, 2014, 63(10): 3497-511.
[3] 富徐燕, 赵丕文, 李亚东,等. 姜黄素对人循环纤维细胞增殖及COLⅠ表达影响的研究[J]. 中国药理学通报, 2014, 30(7): 942-7.
[3] Fu X Y, Zhao P W, Li Y D, et al. Effects of curcumin on proliferation and COL Ⅰ expression of human circulating fibrocytes[J]. Chin Pharmacol Bull, 2014, 30(7): 942-7.
[4] Ferreira M, Valenti V E, Cisternas J R, et al.Correction:memantine effects on liver and adrenal gland of rats exposed to cold stress[J].Int Arch Med,2011,4:7.
[5] Pan J, Li H, Ma J F, et al. Curcumin inhibition of JNKs prevents dopaminergic neuronal loss in a mouse model of Parkinson's disease through suppressing mitochondria dysfunction[J]. Transl Neurodegener, 2012, 1(1):16.
[6] 霍 光,刘 岩. 消瘿瘤合剂对实验性甲状腺肿小鼠FT3及TSH值的影响[J]. 辽宁中医药大学学报, 2010,12(7): 198-9.
[6] Huo G, Liu Y. Effect of XiaoYingLiu mixture on mice's medicine-induced goiter's FT3 and TSH[J].J Liaoning Univ Tradit Chin Med, 2010,12(7): 198-9.
[7] 王 彬, 沈 岚, 从文娟, 等. 基于代谢组学的六味地黄丸干预大鼠甲亢模型的作用研究[J]. 中国药理学通报, 2013, 29(5): 632-7.
[7] Wang B, Shen L, Cong W J, et al. Serum metabonomic study on Liuwei Dihuang pills in the treatment of hyperthyroidism[J]. Chin Pharmacol Bull, 2013, 29(5): 632-7.
[8] Hu G Z, Yang S J, Hu W X, et al. Effect of cold stress on immunity in rats[J]. Exp Ther Med, 2016, 11(1): 33-42.
[9] Xi Y, Li D, San W. Exposure to the endocrine disruptor nonylphenol alters structure and function of thyroid gland in rats[J]. Regul Pept, 2013, 185: 52-6.
[10] 雷升萍, 王 靓, 龙子江, 等. 黄精多糖通过TLR4-MyD88-NF-κB通路抑制缺氧/复氧H9c2心肌细胞炎性因子释放[J].中国药理学通报, 2017, 33(2): 255-60.
[10] Lei S P, Wang L, Long Z J. Inhibitory effect of polygonatum sibiricm polysaccharides on release of inflammatory cytokines of anoxia/reoxyfenation H9c2 myocardial cells through TLR4-MyD88-NF-κB signaling pathway[J]. Chin Pharmacol Bull, 2017, 33(2): 255-60.
[11] 陈 虹, 张闪闪, 董锦蕾, 等. 紫檀茋和乙酰紫草素抑制B16 F10细胞增殖的协同作用[J]. 中国药理学通报, 2016, 32(6): 818-24.
[11] Chen H, Zhang S S, Dong J L, et al. The synergistic antiproliferative effect of pterostilbene and acetylshikonin on B16F10 cells[J]. Chin Pharmacol Bull, 2016, 32(6): 818-24.
[12] Teixeira F, Pollock M, Karim A, et al. Use of antioxidants for the prophylaxis of cold-induced peripheral nerve injury[J].Mil Med, 2002, 167(9): 753-5.
[13] Ishido Y, Luo Y, Yoshihara A, et al. Follicular thyroglobulin enhances gene expression necessary for thyroid hormone secretion[J]. Endocr J, 2015, 62(11): 1077-15.
[14] Mustafa S, Elgazzar A. Influence of chronic exposure to cold environment on thyroid gland function in rabbits[J]. Horm Metab Res, 2014, 46(8): 546-9.
[15] 计 红, 柳巨雄, 杨焕民,等. 冷应激对动物神经内分泌系统影响的研究进展[J]. 环境与健康杂志, 2012, 29(5): 470-2.
[15] Ji H, Liu J X, Yang H M. Research progress on effects of cold stress on main neuroendocrine responses[J]. J Environ Health, 2012, 29(5): 470-2.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(No 81460566,U1603122)
作者简介:李照雪(1992-),女,硕士生,研究方向:中药药理学和系统生物学,E-mail:605576076@qq.com;
张 波(1978-),男,博士,教授,研究方向:系统药理学与中药药理学,通讯作者,E-mail:bozhang_lzu@126.com
更新日期/Last Update: 2017-08-20