[1]马艳霞,吴勉华,姜泽群,等.山茱萸环烯醚萜苷对D-GalN/TNF-α损伤肝细胞的保护作用研究[J].中国药理学通报,2018,(01):118-122.[doi:10.3969/j.issn.1001-1978.2018.01.025]
 MA Yan-xia,WU Mian-hua,JIANG Ze-qun,et al.Protective effect of cornel iridoid glycoside on hepatocytes injured by D-galactosamine/tumor necrosis factor-α[J].Chinese Pharmacological Bulletin,2018,(01):118-122.[doi:10.3969/j.issn.1001-1978.2018.01.025]
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山茱萸环烯醚萜苷对D-GalN/TNF-α损伤肝细胞的保护作用研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年01期
页码:
118-122
栏目:
论著
出版日期:
2018-01-26

文章信息/Info

Title:
Protective effect of cornel iridoid glycoside on hepatocytes injured by D-galactosamine/tumor necrosis factor-α
文章编号:
1001-1978(2018)01-0118-05
作者:
马艳霞1吴勉华2姜泽群3赵凤鸣3李 黎2李沐涵2陆兔林4杭爱武1
南京中医药大学 1.基础医学院中药学教研室、2.第一临床医学院中医内科学教研室、3.医学与生命科学学院分子生物学教研室、4.药学院中药炮制学教研室,江苏 南京 210023
Author(s):
MA Yan-xia1 WU Mian-hua2 JIANG Ze-qun3 ZHAO Feng-ming3 LI Li2LI Mu-han2 LU Tu-lin4 HANG Ai-wu1
1.School of Basic Medicine;
2.First School of Clinical Medicine;
3.School of Medicine and Life Sciences;
4.College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China
关键词:
山茱萸环烯醚萜苷 D-氨基半乳糖 肿瘤坏死因子α 肝损伤 抗氧化 内质网应激 肝保护
Keywords:
cornel iridoid glycoside D-galactosamine tumor necrosis factor-α liver damage anti-oxidation endoplasmic reticulum stress live protection
分类号:
R284.1;R322.47;R329.24;R575.022;R575.01;R977.6
DOI:
10.3969/j.issn.1001-1978.2018.01.025
文献标志码:
A
摘要:
目的 研究山茱萸环烯醚萜苷(CIG)对D-GalN联合TNF-α造成的肝细胞损伤模型的保护作用。方法 提取并分离、制备CIG; 体外培养人源性肝细胞L-02,建立D-GalN/TNF-α致L-02细胞损伤模型,以总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、丙二醛(MDA)、细胞内钙离子浓度为指标,观察CIG对损伤肝细胞的保护作用; Western blot检测p-PERK、p-eIF-2α、caspase-3蛋白的表达,进行机制研究。结果 44 mg·L-1 D-GalN联合100 μg·L-1 TNF-α可作为细胞损伤模型的剂量。CIG高、中、低剂量预保护组可明显提高损伤细胞的活性,明显提高损伤细胞的SOD活性、T-AOC能力,与模型组比较差异具有显著性(P<0.05),降低MDA活性,但低浓度组对MDA活性的降低不明显; Western blot结果显示,CIG不同浓度组可以明显降低内质网应激关联蛋白p-PERK、p-eIF-2α、凋亡关联蛋白caspase-3表达。结论 山茱萸环烯醚萜苷对D-GalN/TNF-α造成的肝细胞损伤具有保护作用,其作用机制可能与增强细胞的抗氧化能力,降低内质网应激造成的损伤,降低凋亡相关蛋白的表达有关。
Abstract:
Aim To determine the effect of cornel iridoid glycoside(CIG)on human hepatocyte cell line(L-02)injured by D-galactosamine(GalN)and tumor necrosis factor-α(TNF-α).Methods Firstly, CIG was extracted, separated and purified.Cell lesion model injured by D-GalN/TNF-α was tested by MTT method.T-AOC, SOD, MDA and calcium ion concentration were taken as indicators to study the effects of CIG on L-02 cell injured by D-GalN/TNF-α.The expression of p-PERK, p-eIF-2α, caspase-3 protein were detected by Western blot.Results 44 mg·L-1 D-GalN and 100 μg·L-1 TNF-α were suitable for L-02 cell lesion model.CIG high, middle, low concentration group could significantly increase the L-02 cell activity by 21%, 13%, 8%, respectively and SOD activity and T-AOC ability as well compared with model group.At the same time, they markedly reduced the MDA activity except the low concentration.Three concentrations of CIG could reduce the expression of endoplasmic reticulum stress related protein PERK, eIF-2α and apoptosis-associated protein caspase-3.Conclusions CIG could protect L-02 cells injured by D-GalN/TNF-α.Increasing the cellular antioxidant ability, reducing the damage of endoplasmic reticulum stress and the expression of apoptosis-associated protein may be the possible mechanism.

参考文献/References:

[1] 国家药典委员会.中华人民共和国药典[S]〗一部.北京: 中国医药科技出版社,2010:26. [1] National pharmacopoeia committee.Pharmacopoeia of the people's republic[S].the first volume.Beijing: Chinese medical science and technology press, 2010:26.
[2] 于 淼,王晓先,贾 琳.山茱萸的药理作用研究进展[J].东南国防医药,2010,12(3): 240-3.
[2] Yu M,Wang X X, Jia L.Research on the pharmacological effects of cornus[J].Southeast Defence Med, 2010,12(3):240-3.
[3] 李观林.山茱萸中环烯醚萜苷类的研究进展[J].亚太传统医药,2014,10(18): 48-9.
[3] Li G L.Research on cornel iridoid glycoside[J]. Asia-Pacific Tradit Med, 2014, 10(18):48-9.
[4] 沈红胜,许慧琴,陆春红.山茱萸环烯醚萜苷类成分对 AGEs 诱导 HUVEC 损伤的保护作用[J].中国药理学通报,2016,32(8):1063-7.
[4] Shen H S,Xu H Q,Lu C H.Protective effect of loganin and morroniside on HUVEC injury induced by advanced glycation end products[J]. Chin Pharmacol Bull,2016,32(8):1063-7.
[5] Silverstein R.D-galactosamine lethality model: scopeand limitations[J]. J Endotoxin Res,2004,10(3):147-62.
[6] Jesephs M D, Bahjar F R, Fukuzuka K, et al.Lipopolysac-charide and D-galactesamine-induced hepatic injury ismediated by TNF-α and not by Fas ligand[J]. Am J Physiol Regul Integr Comp Physiol, 2000, 278(5):1196-201.
[7] Lin H L, Yang J S, Yang J H,et al.The role of Ca2+ on the DADS-induced apoptosis in mouse-rat hybrid retina ganglion cells(N18)[J].Neurochem Res,2006,31(3):383-93.
[8] Lamb H K,Mee C,Xu W, et al.The affinity of a major Ca2+ binding site on GRP78 is differentially enhanced by ADP and ATP[J].J Biol Chem, 2006, 281(13):8796-805.
[9] Muriel P.NF-kappaB in liver diseases:a target for drug therapy [J]. Appl Toxicol, 2009, 29(2):91-100.
[10] Oida Y, Shimazawa M, Imaizumi K, et al.Involvement of endoplasmic reticulum stress in the neuronal death induced by transient forebrain ischemia in gerbil [J]. Neuroscience,2008,151(1):111-9.
[11] Phal H.Signal transduction from the endoplasmic reticulum to the cell nucleus [J]. Physiol Rev,1999,79(3):683-701.

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备注/Memo

备注/Memo:
收稿日期:2017-09-12,修回日期:2017-11-20
基金项目:国家自然科学基金资助项目(No 81373971,81503535); 江苏省高校自然科学基金资助项目(No 13KJB60010)
作者简介:马艳霞(1986-),女,硕士,研究方向:中药药性、配伍机制及应用,E-mail:mayanxia1065@163.com; 杭爱武(1968-),女,博士,副研究员,研究方向:中药药性、配伍机制及应用,通讯作者, E-mail:1340842926@qq.com
更新日期/Last Update: 2017-12-27