[1]余 丹,叶山东,李业琼,等.二甲双胍对2型糖尿病模型大鼠肾功能及肾组织SIRT1表达的影响[J].中国药理学通报,2018,(05):615-619.[doi:10.3969/j.issn.1001-1978.2018.05.007]
 YU Dan,YE Shan-dong,LI Ye-qiong,et al.Effects of metformin on SIRT1 expression of renal tissues in type 2 diabetic rats[J].Chinese Pharmacological Bulletin,2018,(05):615-619.[doi:10.3969/j.issn.1001-1978.2018.05.007]
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二甲双胍对2型糖尿病模型大鼠肾功能及肾组织SIRT1表达的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年05期
页码:
615-619
栏目:
论著
出版日期:
2018-05-26

文章信息/Info

Title:
Effects of metformin on SIRT1 expression of renal tissues in type 2 diabetic rats
文章编号:
1001-1978(2018)05-0615-05
作者:
余 丹1叶山东1李业琼1胡 闻2
安徽医科大学附属省立医院1.内分泌科、2.病理科,安徽 合肥 230001
Author(s):
YU Dan1 YE Shan-dong1 LI Ye-qiong1 HU Wen2
1.Dept of Endocrinology, 2.Dept of Pathology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China
关键词:
2型糖尿病 糖尿病肾功能损害 二甲双胍 SIRT1 格列本脲 足细胞
Keywords:
type 2 diabetes mellitus diabetic nephropathy metformin SIRT1 glibenclamide podocyte
分类号:
R-332; R322.61; R334.1; R341; R587.1; R692; R977.15
DOI:
10.3969/j.issn.1001-1978.2018.05.007
文献标志码:
A
摘要:
目的 观察二甲双胍(MET)对2型糖尿病(T2DM)模型大鼠肾组织沉默调节蛋白1(SIRT1)mRNA和蛋白表达的影响,探讨MET对糖尿病肾功能损害的修复作用机制。方法 30只T2DM模型大鼠随机分为糖尿病组(T2DM组)、格列本脲组(GLY组,5 mg·kg-1·d-1)和二甲双胍组(MET组,300 mg·kg-1·d-1),同时设立正常对照组(NC组)。8周后,观察各组大鼠糖化血红蛋白(HbA1c)、血糖(BG)、尿素氮(BUN)、尿白蛋白排泄和肾小球基底膜厚度(GBMT)情况; 免疫组化检测肾小管SIRT1蛋白表达; real-time PCR检测肾组织SIRT1 mRNA表达; ELISA检测尿SIRT1 蛋白排泄。结果 8周末,MET组及GLY组BG、HbA1c、尿白蛋白/尿肌酐(UACR)、尿SIRT1/尿肌酐(USIR)和GBMT明显低于T2DM组,高于NC组(P<0.05); MET组与GLY组BG、HbA1c和GBMT差异无统计学意义(P>0.05),但UACR明显降低(P<0.05); MET组肾组织SIRT1 mRNA和蛋白表达高于T2DM组,但低于NC组(P<0.05); MET组肾组织SIRT1表达高于GLY组(P<0.05),尿SIRT1蛋白排泄低于GLY组(P<0.05)。结论 二甲双胍可增加T2DM大鼠肾组织SIRT1表达,该作用可能与其肾脏保护有关。
Abstract:
Aim To observe the effects of metformin(MET)on the silencing regulatory protein 1(SIRT1)mRNA and protein expression in renal tissues of type 2 diabetic mellitus(T2DM)rats, and explore its reno-protective mechanisms. Methods Thirty model T2DM rats were randomly divided to glibenclamide intervention group(GLY group, 5 mg·kg-1·d-1), metformin intervention group(MET group, 300 mg·kg-1·d-1)and diabetic control group(T2DM group), and 10 rats with normal glucose tolerance were used as normal control(NC group). After 8 weeks, HbA1c, blood glucose(BG), urea nitrogen(BUN), urinary albumin, creatinine and glomerular basement membrane thickness(GBMT)were detected. The expression of SIRT1 protein in renal tissues was detected by immunohistochemistry, the expression of SIRT1 mRNA in renal tissues was detected by real-time PCR, and urinary SIRT1 protein was detected by ELISA. Results At the end of 8 weeks, the levels of BG, HbA1c, urinary albumin/urinary creatinine(UACR), urinary SIRT1/urinary creatinine(USIR)and GBMT in MET and GLY groups were significantly lower than those in T2DM group(P<0.05). There was no significant difference in BG, HbA1c and GBMT between MET group and GLY group(P>0.05). The expressions of SIRT1 mRNA and protein in MET group were significantly lower than those in NC group(P<0.05), but higher than those in T2DM group(P<0.05). The UACR, expression of SIRT1of renal tissues in MET group was higher than that in GLY group(P<0.05), but urinary SIRT1 protein was lower than that in GLY group(P<0.05). Conclusion Metformin can increase the expressions of SIRT1 in renal tissues of T2DM rats, which may be related to its renal protection.

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备注/Memo

备注/Memo:
收稿日期:2018-01-12,修回日期:2018-02-12
基金项目:安徽省自然科学基金资助项目( No 1508085SMH227)
作者简介:余 丹(1991-),女,硕士生,研究方向:糖尿病及代谢疾病,E-mail:811180965@qq.com;
叶山东(1964-),男,博士,教授,主任医师,博士生导师,研究方向:糖尿病及代谢疾病,通讯作者,E-mail:ysd196406@163.com
更新日期/Last Update: 2018-04-25