[1]张璐莎,陈 璐,李春晓,等.脑心通胶囊对小鼠后下肢急性缺血模型抗炎作用研究[J].中国药理学通报,2018,(12):1767-1773.[doi:10.3969/j.issn.1001-1978.2018.12.028]
 ZHANG Lu-sha,CHEN Lu,LI Chun-xiao,et al.Effect of Naoxintong capsule on inflammation in hind limb ischemia injury in mice[J].Chinese Pharmacological Bulletin,2018,(12):1767-1773.[doi:10.3969/j.issn.1001-1978.2018.12.028]
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脑心通胶囊对小鼠后下肢急性缺血模型抗炎作用研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2018年12期
页码:
1767-1773
栏目:
复方药物药理学
出版日期:
2018-12-10

文章信息/Info

Title:
Effect of Naoxintong capsule on inflammation in hind limb ischemia injury in mice
文章编号:
1001-1978(2018)12-1767-07
作者:
张璐莎陈 璐李春晓尤星宇房志锐宋 敏Joel Wake Coffie 张丽媛王 虹
天津中医药大学天津市现代中药重点实验室-省部共建国家重点实验室培育基地,天津市中药药理学重点实验室,方剂学教育部重点实验室,天津 301617
Author(s):
ZHANG Lu-sha CHEN Lu LI Chun-xiao YOU Xing-yu FANG Zhi-rui SONG Min Joel Wake Coffie ZHANG Li-yuan WANG Hong
Tianjin University of Traditional Chinese Medicine, Tianjin State Key Lab of Modern Chinese Medicine-the Ministry of Education to Build National Key Experimental Cultivation Base, Tianjin State Key Lab of Chinese Pharmacology, Tianjin State Key Lab of Prescription Medicine of Ministry of Education, Tianjin 301617, China
关键词:
脑心通胶囊 后下肢缺血模型 中性粒细胞 巨噬细胞 趋化因子 炎性因子
Keywords:
Naoxintong capsule hind limb ischemia model neutrophils macrophages chemokines inflammatory factors
分类号:
R-332; R282.71; R323.72; R329.2; R364.12; R392.12; R971.1
DOI:
10.3969/j.issn.1001-1978.2018.12.028
文献标志码:
A
摘要:
目的 脑心通胶囊(Naoxintong capsule, NXT)可以有效改善组织缺血症状,但NXT对缺血组织炎性反应的影响及作用机制尚不明确。方法 本研究采用小鼠后下肢缺血模型,研究NXT对缺血组织炎症作用的影响。采用流式细胞术检测给药后3 d,中性粒细胞、巨噬细胞的阳性细胞比例; 采用qRT-PCR检测Emr1、IL-1β、TNF-α的表达; 采用蛋白芯片探究炎症相关蛋白的变化,并对差异蛋白进行生物信息学分析。结果 术后3 d,与模型组相比,NXT组中性粒细胞、巨噬细胞数量明显下调(P<0.01
Abstract:
Aim To explore whether Naoxintong capsule(NXT)can reduce the inflammation after hind limb ischemia(HLI). Methods To investigate the effect of NXT on inflammation after HLI injury in mice, flow cytometry was used to detect the proportion of neutrophils and macrophages on day 3 after surgery. The expression of Emr1 gene was detected by RT-PCR which was an indicator of macrophages, and RT-PCR was used to detect the expression of inflammatory cytokines IL-1β, TNF-α on day 3 after HLI. Proteome ProflerTM Array was carried out to screen and identify the differentially expressed proteins, and the corresponding bioinformatic analysis was done. Results Three days after operation, the number of neutrophils and macrophages in NXT group was significantly smaller than that in model group(P<0.01), and the expression of Emr1 was significantly down-regulated(P<0.01). The expression of IL-1β(P<0.05), TNF-α(P<0.01)in NXT group was significantly lower than that of the untreated group. Compared with model group, NXT up-regulated the expression of IL-4, an inducer of M2a macrophages, as well as CCL17 and CCL22, markers of M2a macrophages, and down-regulated the expression of CCL5, CXCL9, markers of M1 macrophages. Conclusions NXT may reduce the inflammatory response associated with peripheral arterial disease to a certain extent, and provide potential biological markers to a molecular mechanism for anti-inflammatory effect.

参考文献/References:

[1] Criqui M H,Aboyans V. Epidemiology of peripheral artery disease[J]. Circ Res,2015,116(9):1509-26.
[2] Fowkes F,Aboyans V,Fowkes F,et al. Peripheral artery disease: epidemiology and global perspectives[J]. Nat Rev Cardiol,2017,14(3):156-70.
[3] Fowkes F,Rudan D,Rudan I,et al. Comparison of global estimates of prevalence and risk factors for peripheral artery disease in 2000 and 2010:a systematic review and analysis[J]. Lancet,2013,382(9901):1329-40.
[4] Yellon D M,Hausenloy D J. Myocardial reperfusion injury[J]. N Engl J Med,2007,357(11):1121-35.
[5] Turer A T,Hill J A. Pathogenesis of myocardial ischemia-reperfusion injury and rationale for therapy[J]. Am J Cardiol,2010,106(3):360-8.
[6] Zhang M J,Sansbury B E,Hellmann J,et al. Resolvin D2 enhances post-ischemic revascularization while resolving inflammation[J]. Circulation,2016, 134(9):666-80.
[7] Spiller K L,Wrona E A,Romero-Torres S,et al. Differential gene expression in human, murine, and cell line-derived macrophages upon polarization[J]. Exp Cell Res,2016,347(1):1-13.
[8] Mantovani A,Sica A,Sozzani S,et al. The chemokine system in diverse forms of macrophage activation and polarization[J]. Trends Immunol,2004,25(12):677-86.
[9] Das A,Sinha M,Datta S,et al. Monocyte and macrophage plasticity in tissue repair and regeneration[J]. Am J Pathol,2015,185(10):2596-606.
[10] Witherel C E,Yu T,Concannon M,et al. Immunomodulatory effects of human cryopreserved viable amniotic membrane in a pro-inflammatory environment in vitro[J]. Cell Mol Bioeng,2017,10(5):451-62.
[11] Pande R L,Brown J,Buck S,et al. Association of monocyte tumor necrosis factor α expression and serum inflammatory biomarkers with walking impairment in peripheral artery disease[J]. J Vasc Surg,2015,61(1):155-61.
[12] Kyriakides C,Austen W Jr,Wang Y,et al. Skeletal muscle reperfusion injury is mediated by neutrophils and the complement membrane attack complex[J]. Am J Physiol, 1999,277(6 Pt 1):C1263-8.
[13] 于天水,马建伟,官大威,等. 大鼠骨骼肌损伤后不同时间中性粒细胞及巨噬细胞比率的变化[J]. 中国法医学杂志,2015,30(1):16-9.
[13] Yu T S, Ma J W, Guan D W, et al. Changes in the ratio of neutrophils and macrophages at different times after skeletal muscle injury in rats[J]. Chin J Forensic Med,2015,30(1):16-9.
[14] Wang Y,Yan X,Mi S,et al. Naoxintong attenuates ischaemia/reperfusion injury through inhibiting NLRP3 inflammasome activation[J]. J Cell Mol Med,2017,21(1):4-12.
[15] 富莹雪,陈玉萍,卞文青,等. 梓醇对AGEs刺激肾系膜细胞介导巨噬细胞极化的影响[J]. 中国药理学通报,2017,33(10):1399-404.
[15] Fu Y X,Chen Y P,Bian W Q,et al. Effect of catalpol on RAW264.7 macrophage polarization mediated by AGEs-stimulated mouse mesangial cells[J]. Chin Pharmacol Bull, 2017, 33(10):1399-404.

备注/Memo

备注/Memo:
收稿日期:2018-08-02,修回日期:2018-09-10
基金项目:国家国际科技合作专项( No 2015DFA30430 ); 天津市自然科学基金重点项目( No 16JCZDJC36300 ); 长江学者和创新团队发展计划项目( No PCSIRTIRT16R54 ); 国家自然科学基金青年基金项目( No 81603329 )
作者简介:张璐莎(1991-),女,硕士生,研究方向:中药心血管药理学,E-mail:lusha377949511@126.com;
王 虹(1974-),女,博士,研究员,研究方向:中药心血管药理学,通讯作者,E-mail:wanghongsys@126.com
更新日期/Last Update: 2018-12-10