[1]胡 莹,廖云鹏,李福书,等.Wnt10b与骨形态发生蛋白9诱导间充质干细胞成骨分化的关系[J].中国药理学通报,2019,(10):1420-1428.[doi:10.3969/j.issn.1001-1978.2019.10.017]
 HU Ying,LIAO Yun-peng,LI Fu-shu,et al.Relationship between Wnt10b and bone morphogenetic protein-9 induced osteogenic differentiation of mesenchymal stem cells[J].Chinese Pharmacological Bulletin,2019,(10):1420-1428.[doi:10.3969/j.issn.1001-1978.2019.10.017]
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Wnt10b与骨形态发生蛋白9诱导间充质干细胞成骨分化的关系()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1420-1428
栏目:
论著
出版日期:
2019-09-15

文章信息/Info

Title:
Relationship between Wnt10b and bone morphogenetic protein-9 induced osteogenic differentiation of mesenchymal stem cells
文章编号:
1001-1978(2019)10-1420-09
作者:
胡 莹廖云鹏李福书周 娅李 沁孙文娟何百成
重庆市生物化学与分子药理学重点实验室,重庆医科大学药理学教研室,重庆 400016
Author(s):
HU Ying LIAO Yun-peng LI Fu-shu ZHOU Ya LI Qin SUN Wen-juan HE Bai-cheng
Chongqing Key Lab of Biochemistry and Molecular Pharmacology; Dept of Pharmacology, Pharmacy School, Chongqing Medical University, Chongqing 400016, China
关键词:
骨形态发生蛋白9 Wnt10b 间充质干细胞 成骨分化 BMP/Smad信号 成脂分化
Keywords:
bone morphogenetic protein 9 Wnt10b MSCs osteogenic differentiation BMP/Smad signal adipogenic differentiation
分类号:
R-332; R329.24; R341; R336; R394.2; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.10.017
文献标志码:
A
摘要:
目的 研究Wnt10b与骨形态发生蛋白9(BMP9)诱导间充质干细胞(MSCs)骨向分化的关系,以及相关的分子机制。方法 利用PCR、Western blot、组织化学染色等方法,检测BMP9对Wnt10b表达的影响,以及Wnt10b对BMP9诱导的成骨分化的影响。同时,通过定量PCR、Western blot、油红O染色、流式细胞术等,分析Wnt10b影响BMP9成骨分化诱导作用的可能机制。结果 Wnt10b在C3H10T1/2、C2C12、MEFs和MC3T3-E1细胞中均有表达,BMP9在C3H10T1/2细胞中上调Wnt10b的表达水平。Wnt10b增强BMP9在C3H10T1/2细胞中增加OCN蛋白水平和促进钙盐沉积的能力,沉默Wnt10b减弱BMP9的作用。Wnt10b并不改变BMP9对细胞周期的影响,但能增强BMP9诱导的Smad1/5/8磷酸化,沉默Wnt10b减弱BMP9对Smad1/5/8磷酸化的促进作用。此外,Wnt10b抑制BMP9在C3H10T1/2细胞中诱导的成脂分化,沉默Wnt10b则促进BMP9的成脂分化诱导作用。结论 Wnt10b可以促进BMP9诱导的MSCs骨向分化,这种作用可能与增强BMP/Smad信号转导有关。
Abstract:
Aim To study the relationship between Wnt10b and bone morphogenetic protein-9(BMP9)-induced osteogenic differentiation of mesenchymal stem cells(MSCs), and the molecular mechanisms underlying this process.Methods PCR, Western blot and histochemical staining were used to detect the effect of BMP9 on Wnt10b and the effect of Wnt10b on BMP9-induced osteogenic differentiation in MSCs.Meanwhile, real-time PCR, Western blot, oil red O staining, and flow cytometry assay were used to analyze the potential mechanism of Wnt10b affecting the function of BMP9.Results Wnt10b could be detected in C3H10T1/2, C2C12, MEFs and MC3T3-E1 cells.BMP9 up-regulated the expression of Wnt10b in C3H10T1/2 cells.Wnt10b enhanced the capability of BMP9 to increase the level of OCN and mineralization in C3H10T1/2 cells, and silencing Wnt10b attenuated these effects of BMP9.Wnt10b exhibited no substantial effect on cell cycle affected by BMP9, but it enhanced the effect of BMP9 on inducing phosphorylation of Smad1/5/8.While silencing Wnt10b attenuated this effect of BMP9.In addition, Wnt10b inhibited BMP9-induced adipogenic differentiation in C3H10T1/2 cells, and silencing Wnt10b promoted this effect of BMP9.Conclusions Wnt10b can promote BMP9-induced osteogenic differentiation of MSCs, which may be mediated through enhancing BMP/Smad signaling and reducing adipogenic differentiation.

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备注/Memo

备注/Memo:
收稿日期:2019-06-19,修回日期:2019-08-25 基金项目:国家自然科学基金资助项目(No 81572226) 作者简介:胡 莹(1996-),女,硕士生,研究方向:分子药理学,E-mail:1032501281@qq.com; 何百成(1972-),男,博士,教授,博士生导师,研究方向:分子药理学和干细胞生物学,通讯作者,E-mail:cqbche@cqmu.edu.com
更新日期/Last Update: 2019-09-15