[1]潘 峰,田 静,张旭超,等.舒尼替尼对EGFR TKI抵抗的A549 细胞株增殖抑制作用及机制研究[J].中国药理学通报,2011,(08):1121-1125.
 PAN Feng,TIAN Jing,ZHANG Xu-chao,et al. Inhibitory effect of sunitinib on EGFR TK Iˉresistant hum an nonˉsm all cell lung cancer cell line A549and itsm echanism[J].Chinese Pharmacological Bulletin,2011,(08):1121-1125.
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舒尼替尼对EGFR TKI抵抗的A549 细胞株增殖抑制作用及机制研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2011年08期
页码:
1121-1125
栏目:
论著
出版日期:
2011-08-15

文章信息/Info

Title:
 Inhibitory effect of sunitinib on EGFR TK Iˉresistant hum an nonˉsm all c
ell lung cancer cell line A549and itsm echanism
作者:
潘 峰 1 田 静 2 张旭超 3 潘跃银 1
 (1.安徽医科大学第一附属医院肿瘤科,安徽合肥 230022; 2.安徽医科大学公共卫生学院流行病与卫生统计学系,安徽合肥 230032;3.广东省肺癌研究所,广东广州 510080)
Author(s):
 PAN Feng 1 TIAN Jing 2 ZHANG Xu-chao 3 PAN Yue-yin 1
 (1.Dept ofOncology,the FirstAffiliated Hospital ofAnhuiMedicalUniversity,Hefei 230022,China; 2.Dept ofEpidemiology and Biostatistics,School ofPublicHealth,AnhuiMedical
University,Hefei 230032,China; 3.Guangdong Lung Cancer Institute,Guangzhou 510080,China)
关键词:
 舒尼替尼肺癌A549细胞细胞凋亡细胞周期机制Bcl-2
分类号:
R329.24;R329.25;R734.2;R977.3;R979.19
文献标志码:
A
摘要:
 目的 探讨舒尼替尼(sunitinib)体外对表皮生长因子受体酪氨酸激酶抑制剂(EGFR TKI)耐药的非小细胞肺癌A549细胞的生长抑制作用及其机制。方法 MTT法检测sunitinib对A549细胞的生长抑制作用;荧光显微镜观察sunitinib诱导的细胞凋亡;流式细胞术检测sunitinib作用后细胞周期变化;W estern blot检测sunitinib作用后Bclˉ2蛋白水平的变化。结果 在0.4~12.8μmol·L -1 浓度范围内,sunitinib抑制A549细胞增殖,且具有浓度和时间依赖性,24、48、72h的半数抑制浓度(IC 50 )分别为(7.34±0.76)、(5.54±0.62)、(3.68±0.53)μmol·L -1 。荧光显微镜观察发现sunitinib能够诱导细胞出现核固缩、体积缩小等凋亡形态学改变。细胞周期显示sunitinib将A549细胞阻滞于G 0 /G 1 期。W estern blot显示sunitinib能够降低Bclˉ2蛋白的表达水平,且随着sunitinib浓度的升高,Bclˉ2蛋白的表达水平逐渐降低。结论 sunitinib能够抑制EGFR TKI抵抗的A549细胞生长,并具有浓度和时间依赖性,其抗肿瘤的机制可能与诱导细胞周期阻滞和下调Bclˉ2的表达有关。

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备注/Memo

备注/Memo:
收稿日期:2011-03-31,修回日期:2011-05-05
基金项目:安徽省科技厅重点资助项目(No07020304102)
作者简介:潘 峰(1986-),男,硕士生,研究方向:乳腺癌和肺癌的药物治疗,E-mail:amiablepanfeng@163.com;
潘跃银(1965-),男,博士,硕士生导师,教授,主任医师,研究方向:乳腺癌和肺癌的药物治疗,通讯作者,Tel:0551-2922987,Fax:0551-2922987,E-mail:yueyinpan@gmail.com
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