[1]张丽,梁中琴,顾振纶.姜黄素抑制TNFα诱导的BMEC与白细胞的粘附[J].中国药理学通报,2008,(01):63-66.
 ZHANG Li,LIANG Zhong qin,GU Zhen lun.Curcumin inhibits the adherence of leukocytes to BMECs induced by TNFα in vitro[J].Chinese Pharmacological Bulletin,2008,(01):63-66.
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姜黄素抑制TNFα诱导的BMEC与白细胞的粘附()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2008年01期
页码:
63-66
栏目:
论著
出版日期:
2008-01-25

文章信息/Info

Title:
Curcumin inhibits the adherence of leukocytes to BMECs induced by TNFα in vitro
作者:
张丽梁中琴顾振纶
苏州大学医学院药理学教研室,苏州大学衰老和神经疾病实验室,苏州中药研究所,江苏 苏州215123
Author(s):
ZHANG LiLIANG ZhongqinGU Zhenlun
Dept of Pharmacology and Laboratory of Aging and Nervous Diseases, Soochow University School of Medicine; Suzhou Institute of Chinese Materia Medica,Suzhou Jiangsu215123,China
关键词:
姜黄素脑微血管内皮细胞白细胞细胞间粘附分子
Keywords:
curcumin brain microvascular endothelial cells leukocytesICAM
分类号:
R332;R 2841;R 32212
文献标志码:
A
摘要:
目的研究姜黄素(curcumin)对脑微血管内皮细胞(brain microvascular endothelium cell,BMEC)和白细胞粘附的影响。方法采用髓过氧化酶法测定经肿瘤坏死因子(tumor necrosis factorα,TNFα)刺激的BMEC对白细胞的粘附;以RTPCR和Western blot方法检测在TNFα作用下BMEC表面ICAM1表达。结果经TNFα刺激的BMEC明显增加其与白细胞的粘附反应;curcumin(125~100 mg·L-1)可剂量依赖性地抑制TNFα所诱导的BMEC与白细胞的粘附;在TNFα刺激前30 min加入curcumin可抑制BMEC的ICAM1表达。结论curcumin对TNFα所致的BMEC损伤具有保护作用,该作用可能是curcumin下调ICAM1表达,抑制BMEC与白细胞的粘附,从而保护内皮细胞免受损伤。
Abstract:
AimTo investigate the effects of curcumin on the adherence between isolated rat brain microvascular endothelial cells(BMECs)and leukocytes induced by TNFα in vitro.MethodsThe adherence of leukocytes to BMECs and the effects of curcumin were determined by myeloperoxidase. The expression of ICAM1 was detected by RTPCR and immunoblotting.ResultsCurcumin inhibited the TNFα stimulated adherence of leukocytes to BMECs.Pretreament of curcumin also inhibited TNFαinduced increases in the mRNA and protein levels of ICAM1 in BMECs.ConclusionsCurcumin could protect the endothelial cells against damage caused by TNFα.The protective effects of curcumin may be mediated through downregulation of the expression of ICAM1.

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备注/Memo

备注/Memo:
收稿日期:2007-07-24,修回日期:2007-09-20 基金项目:国家自然科学基金资助项目(No 39870945) 作者简介:张丽(1971-),女,博士生,讲师,Email:Lizhang215000@163.com; 梁中琴(1954-),女,教授,博士生导师,研究方向:肿瘤与免疫药理学,通讯作者,Tel:051265880119,Fax:051265190599,Email:liangzhongqin@suda.edu.cn
更新日期/Last Update: 2008-01-15