[1]许灿新,王春,朱炳阳,等.姜黄素通过阻断NFκB减少IL1β诱导的内皮脂酶表达[J].中国药理学通报,2008,(01):75-78.
 XU Can xin,WANG Chun,ZHU Bing yang,et al.Curcumin attenuates IL1βinduced endothelial lipase expression via blocking NFκB pathway[J].Chinese Pharmacological Bulletin,2008,(01):75-78.
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姜黄素通过阻断NFκB减少IL1β诱导的内皮脂酶表达()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2008年01期
页码:
75-78
栏目:
论著
出版日期:
2008-01-25

文章信息/Info

Title:
Curcumin attenuates IL1βinduced endothelial lipase expression via blocking NFκB pathway
作者:
许灿新王春朱炳阳罗其富孙少卫高治平李凯廖端芳
南华大学药物药理研究所,湖南 衡阳421001
Author(s):
XU CanxinWANG ChunZHU BingyangLUO QifuSUN ShaoweiGAO ZhipingLI KaiLIAO Duanfang
Institute of Pharmacy and Pharmacolog School Nanhua University, Hengyang Hunan421001, China
关键词:
内皮脂酶姜黄素核因子κB核因子κB抑制因子α白介素
Keywords:
endothelial lipase curcumin nuclear factorκB inhibitor of nuclear factorκBα IL1β
分类号:
R 28271;R 32212;R 3292
文献标志码:
A
摘要:
目的观察姜黄素对培养的人血管内皮细胞内皮脂酶表达的影响,并探讨其可能的作用机制。方法不同浓度的姜黄素处理HUVEC12细胞。RTPCR检测内皮酯酶(endothelial lipase,EL)mRNA的表达;Western blot检测核因子κB抑制因子α(inhibitor of nuclear factorκBα, IκBα)蛋白的表达;间接免疫荧光检测核因子κB (nuclear factorκB, NFκB)蛋白的活化。结果IL1β处理HUVEC12细胞可以明显上调EL mRNA的表达,同时降低胞质蛋白IκBα的表达水平,激活核转录因子NFκB,增加胞核蛋白NFκB的水平。姜黄素预处理HUVEC12细胞可以抑制IL1β对EL的上调作用,同时逆转IL1β诱导的IκBα蛋白降解和NFκB活化。结论姜黄素可以通过阻断NFκB活化减少IL1β诱导的人血管内皮细胞EL的表达。
Abstract:
AimTo investigate the effect and the possible mechanisms involved in the effect of curcumin on endothelial lipase (EL) expression in human umbilical vein endothelial cells line12 (HUVEC12). MethodsHUVEC12 were pretreated with different concentritions of curcumin (0~100 μmol·L-1 ) and then treated with or without IL1β. Endothelial lipase (EL) expression was detected by RTPCR analysis. The expression of inhibitor of nuclear factorκBα (IκBα) was assayed by Western blot. Nuclear factorκB (NF κB) was analyzed by Immunofluorescence. ResultsIL1β significantly induced EL expression at mRNA level as compared with control group. The IL1β induction of EL expression was accompanied by activation of NFκB and reduction of IκBα. Curcumin almost completely blocked IL1βinduced EL expression with a marked reduction of NFκB and significant induction of IκBα. ConclusionCurcumin inhibited IL1βinduced EL expression via blocking NFκB pathway in HUVEC12 cells.

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备注/Memo

备注/Memo:
收稿日期:2007-08-08,修回日期:2007-10-15 基金项目:国家自然科学基金资助项目(No 30470719);湖南省教委青年重点资助课题(No 99B11);湖南省教育厅科研基金资助项目(No 06C710) 作者简介:许灿新(1975-),男,硕士,讲师,Tel: 07348281408,Email: canxinxu@yahoo.com.cn; 罗其富(1952-),男,教授,研究方向:心血管药理学,通讯作者,Tel: 07348281408,Email:luo.qifu@163.com
更新日期/Last Update: 2008-01-15