[1]王英杰,柴锡庆,韩梅,等.旋覆花内酯抑制AD模型大鼠海马环加氧酶2和核转录因子κB的表达[J].中国药理学通报,2008,(04):0.
 WANG Ying jie,CHAI Xi qing,HAN Mei,et al.Inhibition of lOacetylbritannilactone on expression of COX2 and NFκB in rats hippocampus with AD[J].Chinese Pharmacological Bulletin,2008,(04):0.
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旋覆花内酯抑制AD模型大鼠海马环加氧酶2和核转录因子κB的表达()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2008年04期
页码:
0
栏目:
论著
出版日期:
2008-04-25

文章信息/Info

Title:
Inhibition of lOacetylbritannilactone on expression of COX2 and NFκB in rats hippocampus with AD
作者:
王英杰1柴锡庆12韩梅1温进坤1
1.河北医科大学基础医学研究所省部共建教育部重点实验室,河北 石家庄050017;2.河北化工医药职业技术学院,河北 石家庄050026
Author(s):
WANG Yingjie1CHAI Xiqing12HAN Mei1WEN Jinkun1
1.Key Laboratory by Province and Education Ministry, Institute of Basic Medicine, Hebei Medical University,Shijiazhuang050017,China;2.Hebei Chemical Pharmaceutical College,Shijiazhuang050026,China
关键词:
β淀粉样蛋白阿尔采末病环加氧酶2核转录因子κB旋覆花内酯炎症
Keywords:
βamyloidAlzheimer′s disease cyclooxygenase2nuclear factorκBlOacetylbritannilactone inflammation
分类号:
R332;R 2841;R 32281;R 33864
文献标志码:
A
摘要:
目的观察旋覆花内酯(lOacetylbritannilactone,ABL)对Aβ25~35海马内注射所致阿尔采末病(Alzheimers disease,AD)模型大鼠海马环加氧酶2(COX2),核转录因子κB(NFκB)表达的影响,探讨其对AD大鼠的治疗作用及机制。方法30只SD大鼠随机分为对照组、模型组及药物处理组,Aβ2535海马内注射制备AD大鼠模型,药物处理组给予ABL 26 mg·kg-1·d-1,模型组给予等量溶剂,分两次灌胃,共21 d。用Morris水迷宫测定大鼠的学习记忆能力,免疫组化及Western blot测定大鼠海马COX2,NFκB蛋白表达。结果AD大鼠在定位航行试验中,逃避潜伏期延长,单位时间内跨越原平台次数减少,空间记忆力受损,与对照组及药物处理组大鼠比较差异有统计学意义(P<005);于试验d 2、3、4药物处理组与对照组大鼠比较差异无统计学意义(P>005)。模型组海马COX2、NFκB表达升高(P<005),分别是对照组的28倍和16倍,药物处理组COX2、NFκB表达下降,与模型组比较差异有统计学意义(P<005)。结论ABL的抗炎作用可能是其改善AD大鼠学习记忆能力的作用机制之一。
Abstract:
AimTo explore the molecular mechanism of ABL in rats with AD.MethodsAβ25~35 was injected into bilateral hippocampus to create AD model.Rats were administered by gavage with ABL of 26 mg·kg-1·d-1 for 3 weeks to determine the protective and therapeutic effects on treatment rats. Morris water maze was used to examine spatial and memory performance of rats. The cyclooxygenase2(COX2) and nuclear factorκB (NFκB) protein in hippocampus were detected by immunohistochemistry and Western blot.ResultsAD model rats displayed spatial and memory impairment shown by longer escape latency and less frequency of trying to find the platform(P<005).ABL treatment improved learning and memory of rats with AD and average escape latency between ABLtreated and control rats showed no difference(P>005).Levels of COX2 and NFκB in hippocampus increased approximately 28 and 16 folds respectively in AD model tats than in those of rats with sham operation(P<005), but no differences in levels of COX2 and NFκB were found between ABLtreated and control rats(P>005).ConclusionThe preventive and therapeutic effects of ABL on rats with AD was related to the degression of COX2 and NFκB expression.

参考文献/References:

[1]Han M,Wen J K,Zheng B,et al.Acetylbritannilactone suppresses NO and PGE2 synthesis in RAW 2647 macrophages through the inhibition of iNOS and COX2 gene expression[J].Life Sci,2004,75(6):675-84.
[2]Paxinos G,Watson C. The rat brain in sterotaxic coordinates[M].5th ed. Sydney:Elsevier academic press,2005:102.
[3]Qin L,Liu Y,Cooper C,et al.Microglia enhance betaamyloid peptideinduced toxicity in cortical and mesencephalic neurons by producing reactive oxygen species[J].J Neurochem,2002,83(4):973-83.
[4]Hwang D Y, Kab R, et al. Alterations in behavior, amyloid β42, caspase3, and COX2 in mutant PS2 transgenic mouse model of Alzheimer′s disease[J].FASEB J,2002,12(7):805-13.
[5]Sung S,Yang H X,Uryu K,et al.Modulation of nuclear factorκB activity by indomethacin influences Aβ levels but not Aβ precursor protein metabolism in a model of Alzheimer′s disease[J].Am J Pathol,2004,165(6):2197-206.
[6]Landi F, Cesari M, Onder G,et al.Nonsteroidal antiinflammatory drug(NSAID)use and Alzheimer disease in communitydwelling elderly patients[J].Am J Geriatr Psychiatry,2003,11(2):179-85.
[7]Veld B A,Ruitenberg A, Hofman A,et al.Nonsteroidal antiinflammatory drugs and the risk of Alzheimer′s disease[J].New Engl J Med,2001,345(21):1515-21.
[8]Ferrer I,Marti E, Lopez E,et al.NFκB immonoreactivity is observed in association with beta A4 diffuse plaques in patients with Alzeheimer disease[J].Neuropathol Appl Neurobiol,1998,24(4):271-7.
[9]Yoshiyama Y, Arai K, Hattori T.Enhanced expression of IκB with neurofibrillary pathology in Alzheimer′s disease[J].Neuroreport,2001,12:2641-5.
[10]Tomita S,Fujita T,Kirino Y,et al.PDZ domaindependent suppression of NFκB/p65induced Aβ42 production by a neuronspecific X11like protein[J].J Biol Chem,2000,275(17):13056-60.
[11]陈云波,张大鹏,冯梅,等.人参皂苷Rg1对Aβ25~35诱导的神经细胞核因子κB活化的影响[J].中国药理学通报, 2007,23(5):612-7.
[11]Chen Y B,Zhang D P,Feng M,et al.Effects of Ginsenoside Rg1 on the activation of NFκB in neuronal cells induced by Aβ2535[J].Chin Pharmacol Bull,2007,23(5):612-7.

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备注/Memo

备注/Memo:
收稿日期:2007-11-02,修回日期:2008-01-12基金项目:国家自然科学基金资助项目(No 30472167);河北省自然科学基金资助项目(No C2005000722)作者简介:王英杰(1973-),男,博士生,主治医师,研究方向:老年痴呆,Email:yj310@yahoo.com.cn;柴锡庆(1958-),男,博士,教授,博士生导师,研究方向:老年痴呆,通讯作者,Tel:031185110002,Email:xqchai@163.com
更新日期/Last Update: 2008-04-15