[1]刘华钢,秦三海,刘丽敏,等.鹅掌楸碱银配合物体外诱导肺癌SPCA1细胞凋亡及其机制的研究[J].中国药理学通报,2008,(11):0.
 LIU Hua gang,QIN San hai,LIU Li min,et al.Apoptosisinducing effects of AgLA2 on SPCA1 cells and its mechanism in vitro[J].Chinese Pharmacological Bulletin,2008,(11):0.
点击复制

鹅掌楸碱银配合物体外诱导肺癌SPCA1细胞凋亡及其机制的研究()
分享到:

《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2008年11期
页码:
0
栏目:
论著
出版日期:
2008-11-25

文章信息/Info

Title:
Apoptosisinducing effects of AgLA2 on SPCA1 cells and its mechanism in vitro
作者:
刘华钢1秦三海1刘丽敏1王博龙1刘延成2陈振锋2
1.广西医科大学药学院,广西 南宁530021;2.广西师范大学化学化工学院,广西 桂林541004
Author(s):
LIU Huagang1QIN Sanhai1LIU Limin1WANG Bolong1LIU Yancheng2CHEN Zhenfeng2
1.Pharmacy college of Guangxi Medical University,Nanning530021,China;2.College of Chemistry and Chemical Engineering,Guangxi Normal Universty,Guilin Guangxi541004,China
关键词:
鹅掌楸碱银配合物肺癌细胞凋亡
Keywords:
AgLA2lung carcinomaapoptosis
分类号:
R 2841;R 32924;R 32925
文献标志码:
A
摘要:
目的研究鹅掌楸碱银配合物(AgLA2)体外诱导肺癌SPCA1细胞凋亡作用及其机制。方法采用MTT法, 观察鹅掌楸碱银配合物对肺癌SPCA1细胞增殖的抑制作用,采用DNA ladder检测、细胞形态学观察、酶联免疫法进行凋亡检测;采用RTPCR检测细胞bcl2、bax mRNA表达水平,免疫细胞化学法考察细胞bcl2、bax蛋白的改变。 结果鹅掌楸碱银配合物对肺癌SPCA1细胞的 IC50为3447±0436 mg·L-1。在鹅掌楸碱银配合物的作用下,可见典型的核染色质凝集、碎裂等细胞凋亡的特征;药物作用后细胞中Caspase3含量明显高于对照组;用药组细胞较对照细胞bcl2 mRNA、蛋白表达降低,同时bax mRNA、蛋白表达升高。 结论鹅掌楸碱银配合物可抑制肺癌SPCA1细胞增殖和诱导细胞凋亡,其机制可能是改变bax/ bcl2的比值,改变凋亡的调定点,使得凋亡力量在细胞内占主导优势。
Abstract:
AimTo investigate the apoptosisinducing effects of AgLA2 on lung carcinoma cells SPCA1 and its mechanism in vitro.MethodsThe MTT assay was used to assess the proliferation of SPCA1 cells treated with AgLA2 in vitro.Apoptosisinducing effects was investigated by DNA agarose gel electrophoresis,cell morphology and Elisa.RTPCR was used to measure the expression of bcl2 and bax mRNA,and immunocytochemistry was used to measure the expression of bcl2 and bax protein.ResultsThe IC50 of AgLA2 to SPCA1 cells was (3447±0436) mg·L-1.Treated with AgLA2,typical nuclear chromatine condensation and fragmentation were observed.The concentration of Caspase3 in the group treated with AgLA2 was higher than that of the control group.Treated with AgLA2,bcl2 mRNA,protein expression decreased while bax mRNA,protein expression increased.ConclusionsAgLA2 can inhibit proliferation and induce apoptosis of SPCA1 cells. Its mechanism of action may be related to changing the ratio of bax/bcl2 and the setpoint of apoptosis,making the apoptosis power hold dominance.

参考文献/References:

[1]Zhang Z Z,ElSohly H N,Jacob M R,et al.New sesquiterpenoids from the root of guatteria multivenia[J].Nat Prod,2002,65:856-9.
[2]Chang H C,Chang F R,Wu Y C,et al.Anticancer effect of liriodenine on human lung cancer cells.Kaohsiung[J].Med Sci,2004,20:365-71.
[3]To K K,Wang X,Yu C W,et al.Protein phosphatase 2A inhibition and circumvention of cisplatin crossresistance by novel TCMplatinum anticancer agents containing demethylcantharidin[J].Bioorg Med Chem,2004,12:4565-73.
[4]王艳林,韩钰,袁太宁,等.多胺类似物CPENSpm通过干扰多胺代谢抑制肺癌细胞的增殖[J].中国药理学通报,2008,24(1):132-5.
[4]Wang Y L,Han Y,Yuan T N,et al.Polyamine analogue CPENSpm inhibits proliferation of human lung cancer cells by interfering polyamine metabolism[J].Chin Pharmacol Bull,2008,24(1):132-5.
[5]刘永靖,陈飞虎,于奇,等.腺病毒载体携带人TRAIL基因治疗H446小细胞肺癌的体外实验研究[J].中国药理学通报,2007,23(6):755-9.
[5]Liu Y J,Chen F H,Yu Q,et al.Antitumor activity of replicationdefective adenovirus carrying human tumor necrosis factorrelated apoptosisinducing ligand gene on human small cell lung cancer cell line H446 in vitro[J].Chin Pharmacol Bull,2007,23(6):755-9.
[6]Sinicrope F A,Ruan S B,Cleary K R,et al.bcl2 and p53 oncoproteins expression during colorectal mmorigenesis[J].Cancer Res,1995,55(2):237-41.
[7]Aouad S M,Cohen L Y,Ehsan S A,et al.Caspase3 is a component of Fas death inducing signaling complex in lipid rafts and its activity is required for complete caspase8 activation during Fasmediated cell death[J].Immunology,2004,172:2316-23.
[8]张奕颖,邓涛,胡志芳,等. 熊果酸抑制胃癌细胞SGC7901增殖和诱导细胞凋亡的机制[J]. 癌症,2006,25(4):432-7.
[8]Zhang Y Y,Deng T,Hu Z F,et al.Mechanisms of inhibiting proliferation and inducing apoptosis of human gastric cancer cell line SGC7901 by ursolic acid[J].Chin J Cancer,2006,25(4):432-7.
[9]Murphy K M,Streips U N,Lock R B.bcl2 inhibits a Fasinduced conformational change in the Bax N terminus and Bax mitochondrial translocation[J].J Biol Chem,2000,275:17225-8.
[10]Tari A,Kodama K,Kurihara K,et al.Does serum nitrite concentration reflect gartric carcinogenesis in Japanese Helicobacter pyloriinfected patients[J].Dig Dis Sci,2002,47:100-6.

相似文献/References:

[1]冯文彬,潘雪刁,周捷,等.利用肺癌特异性结合多肽ZS6筛选肺癌相关标志物[J].中国药理学通报,2010,(01):44.
 FENG Wen bin,PAN Xue diao,ZHOU Jie,et al.Screening of tumor mark for the lung cancer by using peptide ZS6[J].Chinese Pharmacological Bulletin,2010,(11):44.
[2]石磊,潘雪刁,王军业,等.噬菌体随机肽库淘选肺癌细胞NCIH1299特异性结合多肽[J].中国药理学通报,2009,(03):0.
 SHI Lei,PAN Xue diao,WANG Jun ye,et al.Panning and identification of lung cancer cell line NCIH1299 specific peptide from a phage display peptide library[J].Chinese Pharmacological Bulletin,2009,(11):0.
[3]郝吉庆,孙耕耘,沈玉先.氯美昔布抗肺癌细胞增殖作用的机制探讨[J].中国药理学通报,2009,(07):0.
 HAO Ji qing,SUN Geng yun,SHEN Yu xian.Mechanisms of lumiracoxib on antitumor in human lung cancer cells[J].Chinese Pharmacological Bulletin,2009,(11):0.
[4]彭蕾,薛仁宇,顾振纶,等.冬凌草甲素对人肺腺癌细胞株SPCA1增殖和凋亡的影响[J].中国药理学通报,2010,(04):558.
 PENG Lei,XUE Ren yu,GU Zhen lun,et al.The inhibitory and apoptosis effect of Oridonin on human lung adenocarcinoma SPCA1[J].Chinese Pharmacological Bulletin,2010,(11):558.
[5]熊飞,詹瑧,唐于平,等.PI3K/Akt信号转导通路在非小细胞肺癌中的作用[J].中国药理学通报,2010,(10):1264.
 XIONG Fei,ZHAN Zhen,TANG Yu ping,et al.The effect of PI3K/Akt signal transduction pathway in nonsmall cell lung cancer[J].Chinese Pharmacological Bulletin,2010,(11):1264.
[6]潘 峰,田 静,张旭超,等.舒尼替尼对EGFR TKI抵抗的A549 细胞株增殖抑制作用及机制研究[J].中国药理学通报,2011,(08):1121.
 PAN Feng,TIAN Jing,ZHANG Xu-chao,et al. Inhibitory effect of sunitinib on EGFR TK Iˉresistant hum an nonˉsm all cell lung cancer cell line A549and itsm echanism[J].Chinese Pharmacological Bulletin,2011,(11):1121.
[7]李济元,王卫华,张彦梅,等.白藜芦醇对肺腺癌细胞增殖和CXCR4表达的影响[J].中国药理学通报,2012,(04):588.
 LI Ji yuan,WANG Wei hua,ZHANG Yan mei,et al.Effects of resveratrol on proliferation and expression of CXCR4 in human lung adenocarcinoma A549 cells[J].Chinese Pharmacological Bulletin,2012,(11):588.
[8]潘雪刁,何冰,王桂香,等.肺癌特异性结合多肽的体外筛选和鉴定[J].中国药理学通报,2013,(03):342.
 PAN Xue diao,HE Bing,WANG Gui xiang,et al.Screening and identifying the peptide specifically binding to lung cancer by using phage display in vitro[J].Chinese Pharmacological Bulletin,2013,(11):342.
[9]韩冬,王贵佐,李满祥.腺苷酸活化蛋白激酶在肺部疾病中的作用[J].中国药理学通报,2013,(04):460.
 HAN Dong,WANG Gui zuo,LI Man xiang.Role of AMPK in pulmonary diseases[J].Chinese Pharmacological Bulletin,2013,(11):460.
[10]李梦姣,等.蓝萼甲素对人肺癌A549细胞的作用及其机制[J].中国药理学通报,2013,(06):882.
 LI Meng jiao,GU Zhen lun,DONG Li xia,et al.Effects of glaucocalyxin A on human lung cancer A549 cells and its mechanism[J].Chinese Pharmacological Bulletin,2013,(11):882.

备注/Memo

备注/Memo:
收稿日期:2007-07-23,修回日期:2008-08-26基金项目:国家自然科学基金地区基金联合资助项目(No 30460153);国家重点基础研究发展计划(973规划)资助项目(No 2007CB516805);广西科学研究与技术开发计划项目(No 桂科攻06300022E);药用资源化学与药物分子工程广西(省部共建教育部)重点实验室资助课题(No 桂科能06300065D03);广西大型仪器协助共用网经费资助作者简介:刘华钢(1956-),女,教授,博士生导师,研究方向:中药新剂型、新制剂及中药药理作用机制,通讯作者,Tel:07715350964,Email:hgliu@263.net
更新日期/Last Update: 2008-11-15