[1]娄宁,汪道峰,方翼,等.云芝多糖B对巨噬细胞膜硫酸软骨素蛋白聚糖结合氧化修饰低密度脂蛋白的影响[J].中国药理学通报,2009,(09):0.
 LOU Ning,WANG Dao feng,FANG Yi,et al.Effects of coriolus versicolor polysaccharides B on macrophage surface chondroitin sulfateproteoglycans binding oxidized low density lipoprotein[J].Chinese Pharmacological Bulletin,2009,(09):0.
点击复制

云芝多糖B对巨噬细胞膜硫酸软骨素蛋白聚糖结合氧化修饰低密度脂蛋白的影响()
分享到:

《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2009年09期
页码:
0
栏目:
论著
出版日期:
2009-09-25

文章信息/Info

Title:
Effects of coriolus versicolor polysaccharides B on macrophage surface chondroitin sulfateproteoglycans binding oxidized low density lipoprotein
作者:
娄宁汪道峰方翼马刚
中山大学附属肿瘤医院重症监护治疗科,华南肿瘤学国家重点实验室,广东 广州510060
Author(s):
LOU NingWANG DaofengFANG YiMA Gang
The Intensive Care Unit, the Affiliated Cancer Hospital of Sun YatSen University Cancer Center,State Key Laboratory of Oncology in Southern China,Guangzhou510060,China
关键词:
云芝多糖蛋白聚糖硫酸软骨素低密度脂蛋白巨噬细胞动脉粥样硬化炎症
Keywords:
coriolus versicolor proteoglycans chondroitin sulfate lowdensity lipoprotein macrophagesatherosclerosisinflammation
分类号:
R 284.1;R 329.24
文献标志码:
A
摘要:
目的探讨野生云芝多糖水溶性新组分CVPSB对RAW2647巨噬细胞膜硫酸软骨素-蛋白聚糖(CSPGs)结合氧化修饰低密度脂蛋白(OxLDL)的影响。方法分别用溴化氰活化的Sepharose CL4B亲和层析及体外结合CSA的方法分别测定CS/PGs与OxLDL的结合率;用离子交换层析提取RAW2647巨噬细胞膜PGs;用1,9二甲基亚甲基蓝(DMMB)分光光度法测定糖胺聚糖。结果在17 μmol MDA·g-1 protein OxLDL水平,不同剂量CVPSB(10、50及100 μg)在体外降低OxLDL(200 μg)与CSA(100 μg)结合,分别降低14%、29%(P<005)及43%(P<001)。不同剂量CVPSB(10、50及100 μg)在体外降低OxLDL(17 μmol MDA·g-1 protein)与巨噬细胞膜表面PGs结合,分别降低8%、27%(P<005)及38%(P<001)。不同剂量CVPSB可抑制OxLDL(50 mg·L-1,17 μmol MDA·g-1 protein)诱导泡沫细胞的形成。结论CVPSB可体外抑制巨噬细胞膜CSPGs结合OxLDL。
Abstract:
AimTo identify the effects of CVPSB on RAW2647 macrophage plasma membrane chondroitin sulfate (CS)proteoglycans (PGs) binging oxidized lowdensity lipoprotein (OxLDL).MethodsRates of PGs binding OxLDL were analyzed by their abilities to bind OxLDL coupled to a CnBractivated Sepharose CL4B chromatography, and rates of CSA binding OxLDL were determined in vitro.RAW2647 macrophages membrane PGs were isolated by anion exchange chromatography.Glycosaminoglycans were analyzed through the DMMB spectrophotometric assay.ResultsAt the level of LDL oxidation (17 μmol MDA·g-1 Pro), however, CVPSB (10, 50, 100 μg) in vitro decreased the ability of CSA (100 μg ) binding OxLDL (200 μg) by 14%, 29% (P<005), and 43% (P<001), respectively. Furthermore, the binding of the PGs to OxLDL (17 μmol MDA·g-1 Pro) was decreased by 8%, 27% (P<005), and 38% (P<001), respectively after CVPSB (10, 50, and 100 μg) being preincubated. CVPSB could inhibit OxLDL (50 mg·L-1,17 μmol MDA·g-1 Pro)induced foam cell formation.ConclusionsThese data provide the first evidence that CVPSB can inhibit the macrophage surface CSPGs binding OxLDL in vitro.

参考文献/References:

[1]娄宁,马刚,汪道峰,等.云芝多糖B抑制血管紧张素Ⅱ诱导的巨噬细胞骨调素基因上调表达[J].中国药理学通报,2008,24(10):1284-8.[1]Lou N,Ma G,Wang D F,et al.Effect of Coriolus versicolor polysaccharide B on osteopontin mRNA expression in macrophages induced by angiotensinⅡ[J].Chin Pharmacol Bull,2008,24(10):1284-8.
[2]娄宁,余学清,祝胜郎,董秀清.消耗还原型谷胱甘肽抑制血管紧张素Ⅱ激活巨噬细胞cJun/ATF2及NFκB[J].中国病理生理杂志,2004,20(10):1745-9.
[2]Lou N,Yu X Q,Zhu S L,Dong X Q.Glutathione depletion inhibits angiotensin Ⅱinduced activation of cJun/ATF2 and NFκB in cultured macrophages[J].Chin J Pathophysiol,2004,20(10):1745-9.
[3]娄宁,马刚,汪道峰,等.云芝多糖B对血管紧张素Ⅱ诱导的巨噬细胞膜糖胺聚糖和细胞内谷胱甘肽含量变化的影响[J].南方医科大学学报,2007,27(12):1824-6.
[3]Lou N,Ma G,Wang D F,et al.Effect of Coriolus Versicolor polysaccharide B on glycosaminoglycans of RAW2647 macrophage plasma membrane and cellular glutathione induced by angiotensin Ⅱ oxidation[J].J South Med Univ,2007,27(12):1824-6.
[4]Kaplan M,Aviram M.Macrophage plasma membrane chondroitin sulfate proteoglycan binds oxidized low density lipoprotein[J].Atherosclerosis,2000,149(1):5-17.
[5]Lou N,Chen Y,Zhou M.Effect of polysaccharide krestin on murine peritoneal macrophage necrosis and foam cell formation induced by oxidized lowdensity lipoprotein[J].Med Sci Res,1996,24(1):49-51.
[6]Chen Y,Zhou M,Lou N.The polysaccharide krestin prevents plaque formation in experimental atherosclerotic rabbits[J].Med Sci Res,1997,25(6):297-300.
[7]陈海生,梁荣能.野生云芝中水溶性多糖的分离鉴定[J].解放军药学学报,2000,16(5):268-70.
[7]Chen H S,Liang R N.Isolation, purification and structural characterizaton of an aqueous polysaccharide from natural Coriolus Versicolor[J].Pharm J Chin PLA,2000,16(5):268-70.
[8]Theocharis A D,Tsolakis I, Tzanakakis G N,et al.Chondroitin sulfate as a key molecule in the development of atheroscleosis and cancer progression[J].Adv Pharmacol,2006,53:281-95.
[9]Gresik W J,Frazier C R,Shapiro J R, et al.Agerelated changes in human bone proteoglycan structure. Impact of osteogenesis imperfecta[J].J Biol Chem,2002,277(46):43638-47.
[10]Ballinger M L,Nigro J,Frontanilla K V,et al.Regulation of glycosaminoglycan structure and atherogenesis[J].Cell Mol Life Sci,2004,61(11):1296-306.
[11]Huang F,Thompson J C,Wilson P G,et al.Angiotensin Ⅱ increase vascular proteoglycan content preceding and contributing to atherosclerosis development[J].J Lipid Res,2008,49(3):521-30.
[12]Chang M Y,Han C Y,Wight T N,Chait A.Antioxidants inhibit the ability of lysophosphatidylcholine to regulate proteoglycan synthesis[J].Arterioscler Thromb Vasc Biol, 2006,26(3):494-500.
[13]Ujita M,Shinomura T,Ito K,et al.Expression and binding activity of the carboxylterminal portion of the core protein of PGM, a large chondroitin sufate proteoglycan[J].J Biol Chem,1994,269(44):27603-9.

备注/Memo

备注/Memo:
收稿日期:2009-05-12,修回日期:2009-06-20基金项目:国家自然科学基金资助项目(No 39700177)作者简介:娄宁(1969-),男,博士,副主任医师,硕士生导师,研究方向:炎症与动脉粥样硬化、组织纤维化,通讯作者,Tel:02087343630,Email:louning@mail.sysu.edu.cn
更新日期/Last Update: 2009-09-25