[1]冯文彬,潘雪刁,周捷,等.利用肺癌特异性结合多肽ZS6筛选肺癌相关标志物[J].中国药理学通报,2010,(01):44-47.
 FENG Wen bin,PAN Xue diao,ZHOU Jie,et al.Screening of tumor mark for the lung cancer by using peptide ZS6[J].Chinese Pharmacological Bulletin,2010,(01):44-47.
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利用肺癌特异性结合多肽ZS6筛选肺癌相关标志物()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2010年01期
页码:
44-47
栏目:
论著
出版日期:
2010-01-25

文章信息/Info

Title:
Screening of tumor mark for the lung cancer by using peptide ZS6
作者:
冯文彬潘雪刁周捷石磊罗军强李询臧林泉
广东药学院药科学院药理学教研室,广东 广州510006
Author(s):
FENG WenbinPAN XuediaoZHOU JieSHI LeiLUO JunqiangLI XunZANG Linquan
Dpet of Pharmacology,Guangdong Pharmacy University,Guangzhou510006,China
关键词:
多肽ZS6cDNA文库减性筛选肺癌肿瘤标志物
Keywords:
peptide ZS6cDNA librarysubtract biopanninglung cancertumor markers
分类号:
R 3423;R 3942;R 7342;R 733;R 734 204;R 734205
文献标志码:
A
摘要:
目的利用本实验室已获得的与肺癌特异性结合的十二肽ZS6从人类肺癌cDNA文库中筛选出肺癌相关标志物,为肺癌的临床早期诊断和靶向治疗奠定基础。方法以生物素标记的十二肽ZS6为探针,筛选人类肺癌cDNA噬菌体展示文库,经过四轮减性筛选后,挑取与ZS6特异性结合的噬菌体单克隆、扩增、纯化,再通过PCR技术扩增、产物纯化后,进行DNA测序,测序结果经过生物信息学分析。结果筛选出18个与ZS6特异性结合的噬菌体克隆,测序结果显示其中1个为未知功能的新基因,其余的均已确定为肿瘤相关基因。结论从人类肺癌cDNA噬菌体展示文库中筛选出潜在的肺癌肿瘤标志物,为肺癌的早期诊治奠定基础。
Abstract:
AimTo select a potential biomarker for early lung cancer diagnosis and targeted therapy by using the cancer specific bounded peptide ZS6 which had already been obtained from the laboratory.MethodsThe peptide ZS6 marked by biotin was used as a probe to pan out the human lung cancer cDNA phage display library,after 4 rounds of subtraction panning,the specific binding clones of ZS6 were identified.After amplification and purification,then those DNA sequences were identified and analyzed with bioinformatics.Results18 phage clones were identified to the specific peptide ZS6 and the DNA sequence showed one of them was an unknown new gene while the others were known tumor related genes.ConclusionA tumor biomarker selected from human lung cancer cDNA library provides a potential tool for early lung cancer diagnosis and therapy.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2009-10-13,修回日期:2009-11-06基金项目:国家自然科学基金资助项目(No 30572177);教育部科学技术研究重点项目(No 208105);广东药学院院内科研启动基金(No 06)和2008师资队伍建设专项经费资助作者简介:冯文彬(1983-),男,硕士生,研究方向:肿瘤药理学,Tel:02039352561,Email:fengbin6688@163.com;臧林泉(1965-),男,教授,研究方向:新药筛选与分子神经药理学、肿瘤药理学,Tel:02039352561,Email:zanglq@yahoo.com
更新日期/Last Update: 2010-01-25