[1]雷军荣,秦军,张晶,等.姜黄素对大鼠缺血性脑损伤炎症反应和血脑屏障通透性的影响[J].中国药理学通报,2010,(01):120-123.
 LEI Jun rong,QIN Jun,ZHANG Jing,et al.Effects of curcumin on inflammatory reaction and bloodbrain barrier permeability in rats following cerebral ischemic injury[J].Chinese Pharmacological Bulletin,2010,(01):120-123.
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姜黄素对大鼠缺血性脑损伤炎症反应和血脑屏障通透性的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2010年01期
页码:
120-123
栏目:
论著
出版日期:
2010-01-25

文章信息/Info

Title:
Effects of curcumin on inflammatory reaction and bloodbrain barrier permeability in rats following cerebral ischemic injury
作者:
雷军荣秦军张晶黄宽明付锐周章明
郧阳医学院附属太和医院,湖北 十堰442000
Author(s):
LEI JunrongQIN JunZHANG JingHUANG KuanmingFU RuiZHOU Zhangming
Taihe Hospital,Yunyang Medical College,Shiyan Hubei442000,China
关键词:
脑缺血姜黄素炎症血脑屏障肿瘤坏死因子α基质金属蛋白酶9
Keywords:
cerebral ischemiacurcumininflammationbloodbrain barriertumor necrosis factorαmatrix metalloproteinase9
分类号:
R332;R 28271;R 32281;R 32924;R 74331022
文献标志码:
A
摘要:
目的研究姜黄素对大鼠脑缺血/再灌注损伤炎症反应和血脑屏障通透性的作用,并进一步探讨其潜在的机制。方法利用线栓法制备大鼠脑缺血/再灌注损伤模型,随机分为对照组、模型组和姜黄素治疗组。检测大鼠神经功能损伤评分和脑梗死体积;通过检测脑组织髓过氧物酶的含量说明中性粒细胞浸润和炎症反应的程度;检测脑组织伊文思蓝的含量说明血脑屏障的破坏程度;ELISA检测脑组织肿瘤坏死因子α(TNFα)的含量;免疫印记法检测基质金属蛋白酶9(MMP9)的表达变化。结果姜黄素治疗组减轻神经功能损伤、脑梗死体积,并减轻中性粒细胞在脑组织的浸润程度、改善血脑屏障完整性。同时姜黄素还可以降低脑组织TNFα的含量以及MMP9的表达水平。结论姜黄素通过减轻炎症反应和血脑屏障破坏对大鼠脑缺血/再灌注损伤起脑保护作用。姜黄素的脑保护作用可能与其降低TNFα含量和MMP9的表达有关。
Abstract:
AimTo explore the effects of curcumin on inflammatory reaction and bloodbrain barrier permeability in rats following cerebral ischemic injury,and to further investigate its potential mechanisms.MethodsSD rats underwent the cerebral ischemia/reperfusion injury by the sutrure occlusion model were randomly divided into shamcontrol,cerebral ischemia and curcumintreatment groups.Neurological deficit scores,cerebral infarction volume,brain water content and bloodbrain barrier (BBB) permeability were measured,myeloperoxidase(MPO)activities in rat brain were measured as an index of neutrophil infiltration;content of tumor necrosis factorα(TNFα)in rat brain was detected by ELISA;expression of matrix metalloproteinase9(MMP9) in rat brain was determined by Western blot.ResultsNeurological deficit and cerebral infarction volume was decreased in curcumintreatment group.The degree of neutrophilicgranulo cyte infiltration in cerebral tissues was decreased and the integrity of BBB was improved.Curcumin could also inhibit TNFα and MMP9 expression.ConclusionCurcumin exerts the neuroprotective effect on cerebral ischemia/reperfusion injury in rats through inhibiting the inflammatory reaction and improving BBB integrity,which may be associated with the inhibiton of TNFα and MMP9.

参考文献/References:

[1]Dirnagl U,Iadecola C,Moskowitz M A.Pathobiology of ischaemic stroke:an integrated view[J].Trends Neurosci,1999,22(9):391-7.
[2]Lin H Y,Huang C C,Chang K F.Lipopolysaccharide preconditioning reduces neuroinflammation against hypoxic ischemia and provides longterm outcome of neuroprotection in neonatal rat[J].Pediatr Res,2009,66(3):254-9.
[3]Tu X K,Yang W Z,Shi S S,et al.5lipoxygenase inhibitor zileuton attenuates ischemic brain damage:involvement of matrix metalloproteinase 9[J].Neurol Res,2009,31(8):848-52.
[4]Zhao J,Zhao Y,Zheng W,et al.Neuroprotective effect of curcumin on transient focal cerebral ischemia in rats[J].Brain Res,2008,1229:224-32.
[5]周瑞,徐春红,李军,等.姜黄素对缺血/再灌注大鼠海马神经细胞凋亡及NR2A、NR2B表达的影响[J].中国药理学通报,2008,24(10):1314-8.
[5]Zhou R,Xu C H,Li J,et al.Effects of curcumin on the expressions of NR2A,NR2B and apoptotic neurons in hippocampus after ischemiareperfusion in rats[J].Chin Pharmacol Bull,2008,24(10):1134-8.
[6]Longa E Z,Weinstein P R,Carlson S,Cummins R.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
[7]Huang Z,Huang P L,Panahian N,et al.Effects of cerebral ischemia in mice deficient in neuronal nitric oxide synthase[J].Science,1994,265(5180):1883-5.
[8]Aggarwal B B,Kumar A,Bharti A C.Anticancer potential of curcumin:preclinical and clinical studies[J].Anticancer Res,2003,23(1A):363-98.
[9]Motterlini R,Foresti R,Bassi R,Green C J.Curcumin,an antioxidant and antiinflammatory agent,induces heme oxygenase1 and protects endothelial cells against oxidative stress[J].Free Radic Biol Med,2000,28(8):1303-12.
[10]Tu X K,Yang W Z,Shi S S,et al.Neuroprotective effect of baicalin in rat model of permanent focal cerebral ischemia[J].Neurochem Res,2009,34(9):1626-34.
[11]Kelly M A,Shuaib A,Todd K G.Matrix metalloproteinase activation and bloodbrain barrier breakdown following thrombolysis[J].Exp Neurol,2006,200(1):38-49.
[12]欧阳昌汉,吴基良,贺震华,等.罗格列酮对大鼠脑缺血/再灌注损伤的保护作用[J].中国药理学通报,2007,23(1):110-4.
[12]Ouyang C H,Wu J L,He Z H,et al.Protective effect of rosiglitazone on cerebral ischemia/reperfusion injury in rats[J].Chin Pharmacol Bull,2007,23(1):110-4.

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备注/Memo

备注/Memo:
收稿日期:2009-09-22,修回日期:2009-10-27作者简介:雷军荣(1975-),男,硕士,主治医师,讲师,研究方向:缺血性脑损伤的机制,Tel:07198801487,Email:ljrong0005@sina.com;秦军(1969-),男,博士,副主任医师,副教授,研究方向:缺血性脑损伤的机制,通讯作者,Tel:07198801487,Email:herono@163.com
更新日期/Last Update: 2010-01-25