[1]胡莹,焦海霞,王瑞幸,等.三七皂苷R1对肺高压大鼠模型肺动脉的舒张作用[J].中国药理学通报,2013,(11):1572-1576.
 HU Ying,JIAO Hai xia,WANG Rui xing,et al.Vasodilation of notoginsenoside R1 on pulmonary arteries of pulmonary hypertensive rats[J].Chinese Pharmacological Bulletin,2013,(11):1572-1576.
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三七皂苷R1对肺高压大鼠模型肺动脉的舒张作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2013年11期
页码:
1572-1576
栏目:
论著
出版日期:
2013-11-11

文章信息/Info

Title:
Vasodilation of notoginsenoside R1 on pulmonary arteries of pulmonary hypertensive rats
作者:
胡莹焦海霞王瑞幸郭立成穆云萍林默君
福建医科大学基础医学院生理学与病理生理学系,心血管科学研究室,福建 福州350108
Author(s):
HU Ying JIAO Haixia WANG Ruixing GUO Licheng MU Yunping LIN Mojun
Dept of Physiology and Pathophysiology, Laboratory of Cardiovascular Science, Fujian Medical University, Fuzhou Fujian350108, China
关键词:
慢性低氧野百合碱肺高压三七皂苷R1钙池操纵性钙内流肺动脉
Keywords:
chronic hypoxia monocrotaline pulmonary hypertension notoginsenoside R1 storeoperated Ca2+ entry pulmonary artery
分类号:
R-332;R284.1;R32212;R363-332;R544022;R9724
文献标志码:
A
摘要:
目的探讨三七皂苷R1(notoginsenoside R1)对慢性低氧(chronic hypoxia, CH)和野百合碱(monocrotaline,MCT)致肺高压(pulmonary hypertension,PH) 大鼠模型肺动脉(pulmonary arteries,PAs)收缩效应的作用及其机制。方法清洁级♂ SD大鼠随机分为3组。正常对照组;CH组:大鼠饲养于氧分压为100%±05% 的密封有机玻璃箱内;MCT组:大鼠按60 mg·kg-1 (BW) 一次性腹腔注射20 mg·L-1 的MCT。观察三七皂苷R1对两种肺高压模型大鼠PAs收缩效应的作用。结果可成功制备CH和MCT致肺高压大鼠;三七皂苷R1(01~100 μmol·L-1)对10 nmol·L-1内皮素1(endothelin1,ET1)诱发正常大鼠PAs收缩效应呈剂量依赖性舒张,EC50为(525±014) μmol·L-1;3 nmol·L-1 Gd3+阻断ET1诱发两种肺高压模型大鼠PAs收缩效应后,再加入三七皂苷R1无进一步抑制作用;6 μmol·L-1三七皂苷R1对环匹阿尼酸(cyclopiazonic acid,CPA)诱发两种肺高压模型大鼠PAs收缩效应均有明显的抑制作用。结论三七皂苷R1可能通过阻断钙池操纵性钙内流介导的PAs收缩产生治疗肺高压作用。
Abstract:
AimTo evaluate the effects of notoginsenoside R1 on isolated pulmonary arteries (PAs) of chronic hypoxia (CH) and monocrotaline (MCT)treated rats and its underlying mechanisms.MethodsTension of PA rings was examined in CH exposure(100%±05%/partial pressure of oxygen) and MCTinjection (60 mg·kg-1) induced pulmonary hypertensive (PH) rats. Results(1)CH and MCTtreated rats exhibited profound PH and right ventricular hypertrophy when examined 3 weeks after hypoxia exposure or MCT injection, respectively. (2)Notoginsenoside R1 inhibited the ET1 induced PAs contraction of control rats in a dosedependent manner. EC50 was (525±014) μmol·L-1. (3) 6 μmol·L-1 notoginsenoside R1 showed no significant effects after 3 nmol·L-1 Gd3+ induced relaxation on 10 nmol·L-1 ET1 precontracted PAs in CH and MCTinduced rats. (4) 10 μmol·L-1 CPAinduced PAs constrictions were partially inhibited by 6 μmol·L-1 notoginsenoside R1 in CH and MCT groups. ConclusionNotoginsenoside R1 prevents PH development likely by blunting storeoperated Ca2+ entry induced vasoconstriction.

参考文献/References:

[1]Simonneau G. A new clinical classification of pulmonary hypertension [J].Bull Acad Natl Med, 2009, 193(8): 1897-909.
[2]张明芳,刘晓如,杨娜. TRPC6 介导肺动脉高压大鼠肺动脉张力和肺动脉平滑肌细胞Ca2+浓度的升高[J]. 生理学报, 2010,62(1):55-62.
[2]Zhang M F, Liu X R, Yang N. TRPC6 mediates the enhancements of pulmonary arterial tone and intracellular Ca2+ concentration of pulmonary arterial smooth muscle cells in pulmonary hypertension rats[J]. Acta Physiol Sin, 2010, 62(1): 55-62.
[3]Humbert M, Morrell N W, Archer S L. Cellular and molecular pathobiology of pulmonary arterial hypertension[J].J Am Coll Cardiol, 2004, 43(12 Suppl S): 13S-24S.
[4]Lin M J, Leung G P, Zhang W M. Chronic hypoxiainduced upregulation of storeoperated and receptoroperated Ca2+ channels in pulmonary arterial smooth muscle cells: a novel mechanism of hypoxic pulmonary hypertension[J].Circ Res, 2004, 95(5): 496-505.
[5]Liu X R, Zhang M F, Yang N. Enhanced storeoperated Ca2+ entry and TRPC channel expression in pulmonary arteries of monocrotalineinduced pulmonary hypertensive rats[J].Am J Physiol Cell Physiol, 2012, 302(1): C77-87.
[6]Wang J, Jiang Q, Wan L. Sodium tanshinone IIA sulfonate inhibits canonical transient receptor potential expression in pulmonary arterial smooth muscle from pulmonary hypertensive rats[J].Am J Respir Cell Mol Biol, 2013, 48(1): 125-34.
[7]Wang C Z, Xie J T, Zhang B. Chemopreventive effects of Panax notoginseng and its major constituents on SW480 human colorectal cancer cells[J].Int J Oncol, 2007, 31(5): 1149-56.
[8]Liu X R, Liu Q, Chen G Y. Downregulation of TRPM8 in pulmonary arteries of pulmonary hypertensive rats[J]. Cell Physiol Biochem, 2013,31(6): 892-904.
[9]Chen W X, Wang F, Liu Y Y. Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion[J].World J Gastroenterol, 2008, 14(1): 29-37.
[10]Sun B, Xiao J, Sun X B. Notoginsenoside R1 attenuates cardiac dysfunction in endotoxemic mice: an insight into oestrogen receptor activation and PI3K/Akt signalling[J].Br J Pharmacol, 2013, 168(7): 1758-70.
[11]林默君, 关永源, 贺华. 巯亚硝基卡托普利对环匹阿尼酸引起的平滑肌细胞胞浆游离Ca2+浓度升高作用的影响[J]. 中国药理学通报, 2001, 17(6):632-7.
[11]Lin M J, Guan Y Y, He H. Effect of Snitrosocaptopril on cyclopiazonic acidinduced [Ca2+]i change in rat aortic smooth muscle cells[J]. Chin Pharmacol Bull,2001,17(6):632-7.
[12]承伟,李智. 肺动脉高压模型大鼠肺动脉平滑肌细胞内钙的改变[J]. 中国药理学通报, 2010, 26(5): 593-6.
[12]Cheng W, Li Z. Alteration of [Ca2+]i in pulmonary artery smooth muscle of pulmonary artery hypertension rats[J]. Chin Pharmacol Bull, 2010, 26(5): 593-6.

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备注/Memo

备注/Memo:
收稿日期:2013-06-13,
修回日期:2013-07-10
基金项目:国家自然科学基金资助项目(No 31171104);福建省自然科学基金资助项目(No C1010116);福建医科大学重大科研课题资助项目(No 09ZD010)
作者简介:胡莹(1986-),女,硕士,研究方向:肺高压机制研究,Tel:059122862429,Email:271031641@qq.com; 林默君(1964-),男,博士,教授,研究方向:肺高压机制研究,通讯作者,Tel:059122862429,Email:mjlin@mail.fjmu.edu.cn
更新日期/Last Update: 2013-11-11