[1]张 哲,王 超.KLF4在糖尿病小鼠心肌组织的表达及通心络胶囊干预作用研究[J].中国药理学通报,2015,(06):876-881.
 ZHANG Zhe,WANG Chao.Expression of KLF4 in diabetic mouse myocardium and the interventional effect of Tongxinluo capsule on diabetic cardiomyopathy[J].Chinese Pharmacological Bulletin,2015,(06):876-881.
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KLF4在糖尿病小鼠心肌组织的表达及通心络胶囊干预作用研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2015年06期
页码:
876-881
栏目:
复方药物药理学
出版日期:
2015-06-15

文章信息/Info

Title:
Expression of KLF4 in diabetic mouse myocardium and the interventional effect of Tongxinluo capsule on diabetic cardiomyopathy
作者:
张 哲王 超
河北省人民医院省老年医学重点实验室,河北 石家庄 050051
Author(s):
ZHANG ZheWANG Chao
People's Hospital of Hebei Province,Key Laboratory of Geriatric Medicine,Shijiazhuang 050051,China
关键词:
通心络胶囊 糖尿病心肌病 Krüppel样因子4 血糖 血脂 NF-κB 炎症 信号通路
Keywords:
Tongxinluo capsule diabetic cardiomyopathy Krüppel-like factor 4 blood sugar blood lipids NF-κB inflammation signal pathway
分类号:
R-332; R287; R322.11; R394.2; R587.202.2; R587.201
文献标志码:
A
摘要:
目的 探讨糖尿病小鼠心肌组织Krüppel样因子4(KLF4)表达及通心络(TXL)对其干预作用。方法 KK/Upj-Ay小鼠40只,随机分为糖尿病模型组、TXL低、中、高剂组,每组10只,另设C57BL/6(C57)小鼠10只作为对照组。按照分组给予不同剂量处理因素,疗程3个月,测定各组空腹血糖(FBG)、糖化血红蛋白(HbA1c)、甘油三酯(TG)、总胆固醇(TC),空腹胰岛素(FINS)、计算胰岛素抵抗指数(HOMA-IR); 放射免疫法测定各组血清TNF-α、IL-6水平; HE染色观察心肌组织病理变化; Real time-PCR和Western blot测定心肌组织KLF4 mRNA和蛋白表达; Western blot测定心肌组织核因子-κB(nuclear factor kappa B,NF-κB)核蛋白表达。 结果 与对照组比较,模型组FBG、HbA1c、TG、TC、FINS、HOMA-IR及TNF-α、IL-6水平明显增高,KLF4 mRNA和蛋白表达明显降低,NF-κB核蛋白表达明显升高(P<0.01),通心络胶囊能够明显减轻心肌组织病理损伤,降低FINS、TG、TC、HOMA-IR、TNF-α、IL-6水平及NF-κB核蛋白表达,同时上调KLF4表达(P<0.05),但不影响FBG、HbA1c(P>0.05)。 结论 KLF4可能参与了糖尿病心肌病的发生发展。通心络胶囊可减轻糖尿病心肌病理损伤及改善心功能,其机制可能与上调KLF4表达及抑制NF-κB炎症信号通路的激活有关。
Abstract:
Aim To observe the expressions of KLF4 in the myocardium of diabetic mice and their changes under Tongxinluo capsule intervention.Methods Forty KK/Upj-Ay mice were randomly divided into diabetic model group(n=10)and diabetic model with Tongxinluo(TXL low,middle,high)groups(n=10,respectively).C57BL/6 mice were selected as control group(n=10). At the end of the 3th month the mice were sacrificed and were weighed. The fasting blood-glucose(FBG),glycosylated hemoglobin(HbA1c),triglyceride(TG),total cholesterol(TC)and insulin(FINS)were measured to calculate HOMA-IR. Radioimmunoassay was used to measure serum TNF-α and IL-6. The pathological changes in the myocardium of mice were observed by HE staining. KLF4 mRNA was examined by Real-time PCR,while KLF4 and NF-κB protein were measured by Western blot.Results Compared to the control group,FBG,HbA1c,TG,TC,FINS,HOMA-IR,TNF-α and IL-6 in model group were markedly increased; the expressions of myocardial KLF4 were markedly decreased and the expression of nuclear NF-κB protein were markedly increased(P<0.01). Tongxinluo capsule could significantly reduce myocardial pathological damage,FINS,TG,TC,TNF-α,IL-6 level and the expression of nuclear NF-κB protein and up-regulate the expression of KLF4(P<0.05),but had no effect on FBG and HbA1c(P>0.05).Conclusions KLF4 may be involved in the development of myocardial injury during diabetes. Tongxinluo capsule can ameliorate the myocardial damage and improve the function of diabetic myocardium by up-regulating the expression of KLF4 and inhibiting the activation of NF-κB inflammatory signal pathway.

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备注/Memo

备注/Memo:
收稿日期:2015-02-22,修回日期:2015-04-05 基金项目:河北省自然科学基金资助项目(No C2011307008) 作者简介:张 哲(1980-),女,博士,助理研究员,研究方向:代谢综合征、糖尿病、血脂紊乱和骨质疏松等疾病发病机制,Tel:0311-85988007,E-mail:zhe_zhang80@126.com; 王 超(1975-),男,博士,副研究员,研究方向:代谢综合征、糖尿病、血脂紊乱和骨质疏松等疾病发病机制,通讯作者,Tel:0311-85988310,E-mail:13731156811@139.com
更新日期/Last Update: 1900-01-01