[1]徐天娇,刘 勇,李 萍,等.泛素连接酶Cbl-b调控p38MAPK在胰岛素与硒协同抑制糖尿病心肌病大鼠心肌细胞凋亡中的作用[J].中国药理学通报,2016,(08):1170-1174.[doi:10.3969/j.issn.1001-1978.2016.08.027]
 XU Tian-jiao,LIU Yong,LI Ping,et al.Role of ubiquitin ligase Cbl-b-regulated p38MAPK in insulin and selenium synergistic anti-myocardial apoptosis in diabetic cardiomyopathy[J].Chinese Pharmacological Bulletin,2016,(08):1170-1174.[doi:10.3969/j.issn.1001-1978.2016.08.027]
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泛素连接酶Cbl-b调控p38MAPK在胰岛素与硒协同抑制糖尿病心肌病大鼠心肌细胞凋亡中的作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2016年08期
页码:
1170-1174
栏目:
论著
出版日期:
2016-08-15

文章信息/Info

Title:
Role of ubiquitin ligase Cbl-b-regulated p38MAPK in insulin and selenium synergistic anti-myocardial apoptosis in diabetic cardiomyopathy
文章编号:
1001-1978(2016)08-1170-05
作者:
徐天娇1刘 勇2李 萍1胥晓丽1曾菊绒1
1. 西安医学院药理学与毒理学教研室,陕西 西安 710021;
2. 延安大学咸阳医院老年病科,陕西 咸阳 712000
Author(s):
XU Tian-jiao1 LIU Yong2 LI Ping1 XU Xiao-li1 ZENG Ju-rong1
1. Dept of Pharmacology, Xi'an Medical University, Xi'an 710021,China;
2. Dept of Geriatrics, Xianyang Hospital of Yanan University,Xianyang Shanxi 712000,China
关键词:
胰岛素 糖尿病心肌病 凋亡 Cbl-b p38 丝裂原活化蛋白激酶 Ku70
Keywords:
insulin selenium diabetic cardiomyopathy apoptosis Cbl-b p38MAPK Ku70
分类号:
R-332;R322.11;R329.25;R347.8;R542.2;R587.2;R916.3
DOI:
10.3969/j.issn.1001-1978.2016.08.027
文献标志码:
A
摘要:
目的 初步探讨胰岛素和硒协同抑制糖尿病心肌病大鼠心肌细胞凋亡的机制。方法 SD大鼠50只,随机分为空白对照组(Control组)、糖尿病心肌病模型组(DCM组)、糖尿病心肌病+胰岛素组(DCM+In组)、糖尿病心肌病+硒组(DCM+Se组)、糖尿病心肌病+胰岛素+硒组(DCM+In+Se组)。TUNEL法观察心肌细胞凋亡; Western blot观察凋亡相关蛋白Bcl-2、caspase-3和PARP剪切片段的变化,同时观察Cbl-b和p38MAPK表达变化; 免疫沉淀法检测Cbl-b与p38MAPK、Ku70与Bax之间的相互作用。结果 胰岛素与硒协同抑制心肌细胞凋亡(P<0.01); 胰岛素联合硒协同,增加Cbl-b的表达,降低p38MAPK表达(P<0.01); 两药联合协同增加Cbl-b与p38MAPK、Ku70与Bax之间的相互作用(P<0.01)。结论 胰岛素与硒通过调控Cbl-b,抑制p38MAPK而阻止Bax转位来协同抑制心肌细胞凋亡。
Abstract:
Aim To explore the mechanism of insulin in combination with selenium preventing myocardial apoptosis in diabetic cardiomyopathy rats.Methods SD rats(n=50)were randomly divided into five groups: control, diabetic cardiomyopathy(DCM), DCM with insulin treatment, DCM with selenium treatment, and DCM with insulin and selenium combination treatment. The cell apoptosis was observed by TUNEL. The levels of Bcl-2, caspase-3,PARP,Cbl-b and p38MAPK were examined by Western blot. The interactions of Cbl-b-p38MAPK and Ku70-Bax were detected by immunoprecipitation. Results Insulin in combination with selenium synergistically inhibited apoptosis,up-regulated Cbl-b,down-regulated p38MAPK expressions and increased the interactions of Cbl-b-p38MAPK and Ku70-Bax.Conclusion Insulin and selenium synergistically inhibit myocardial apoptosis by regulating Cbl-b-inhibited p38MAPK and preventing Bax translocation.

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备注/Memo

备注/Memo:
基金项目:陕西省教育厅自然科学项目(No 15JK1633); 西安医学院重点学科建设经费资助
作者简介:徐天娇(1975-),女,博士,副教授,研究方向:糖尿病及其发病机制,Tel:029-86177562,E-mail:xtj1118@163.com
更新日期/Last Update: 2016-08-15