[1]马 瑶,周 童,张海楼,等.越鞠丸对皮质酮模型小鼠抑郁样行为和神经新生的影响[J].中国药理学通报,2019,(02):283-288.[doi:10.3969/j.issn.1001-1978.2019.02.027]
 MA Yao,ZHOU Tong,ZHANG Hai-lou,et al.Effect of Yueju pill on depressive-like behavior and neurogenesis in corticosterone induced mice[J].Chinese Pharmacological Bulletin,2019,(02):283-288.[doi:10.3969/j.issn.1001-1978.2019.02.027]
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越鞠丸对皮质酮模型小鼠抑郁样行为和神经新生的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年02期
页码:
283-288
栏目:
复方药物药理学
出版日期:
2019-02-14

文章信息/Info

Title:
Effect of Yueju pill on depressive-like behavior and neurogenesis in corticosterone induced mice
文章编号:
1001-1978(2019)02-0283-06
作者:
马 瑶124周 童12张海楼1严瑶瑶12王 薇3薛文达14王福顺3
南京中医药大学1.基础医学院转化系统生物学和神经科学研究中心,中医脑病重点实验室, 2.医学与生命科学学院,3.心理学院,江苏 南京 210023; 4.南通大学神经再生协同创新中心,江苏 南通 226001
Author(s):
MA Yao124 ZHOU Tong12 ZHANG Hai-lou1 YAN Yao-yao12 WANG Wei3 XUE Wen-da14 WANG Fu-shun3
1.Center for Translational Systems Biology and Neuroscience, Key Lab of Integrative Medicine for Brain Diseases,School of Basic Biomedical Science, 2.School of Medicine and Life Sciences, 3.School of Psychology, Nanjing University of Chinese Medicine, Nanjing 210023, China; 4.Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, China
关键词:
越鞠丸 皮质酮 抗抑郁 海马 神经新生 PKA-ERK-CREB信号通路
Keywords:
Yueju pill corticosterone antidepression hippocampus nerve regeneration PKA-ERK-CREB signaling pathway
分类号:
R-332; R289.5; R322.81; R329.25; R749.42; R977.1
DOI:
10.3969/j.issn.1001-1978.2019.02.027
文献标志码:
A
摘要:
目的 探讨越鞠丸对慢性皮质酮诱导小鼠的抑郁样行为及神经新生的作用。方法 C57BL/6N小鼠进行皮质酮注射造模及给药,行为学检测模型及药效,免疫组化法比较小鼠海马DG区Ki67+细胞数量,蛋白免疫印迹法检测PKA-ERK-CREB信号通路的表达变化。结果 糖水偏好结果显示,皮质酮诱导小鼠糖水偏好程度明显下降,越鞠丸则能逆转这一表型; 悬尾测试和陌生环境摄食测试的结果与糖水偏好结果相类似。免疫组化结果显示,皮质酮诱导小鼠海马DG区Ki67+细胞数量明显减少,而越鞠丸能够增加阳性细胞数量。蛋白免疫印迹结果显示,皮质酮诱导小鼠海马中PKA、CREB、ERK的表达水平均明显下调,而越鞠丸则可以上调这些蛋白表达。结论 在皮质酮诱导抑郁模型小鼠中,越鞠丸通过激活PKA-ERK-CREB信号通路,进而增强海马神经新生,从而起到抗抑郁的作用。
Abstract:
Aim To investigate the effects of Yueju pill on depressive-like behavior and neurogenesis in chronic corticosterone(CORT)induced mice.Methods Male C57BL/6N mice received chronic administration of CORT as well as Yueju pill.Behavioral tests were performed after the completion of treatment.The number of Ki67+ cells in the hippocampal DG region of each group was counted by immunohistochemistry test.Meanwhile, the expression levels of PKA, ERK and CREB were detected by Western blot.Results The sucrose preference notably decreased(P<0.05)in CORT-induced mice in SPT, and it was raised obviously after Yueju treatment.The TST and NSF test displayed the similar results.The immunohistochemistry showed that the number of Ki67+ cells in the hippocampus DG significantly decreased in CORT-induced mice, while the number of Yueju group apparently increased(P<0.05).Western blot showed that compared with control group, the protein expressions of PKA-ERK-CREB pathway were down-regulated in CORT-induced mice, which were up-regulated after Yueju treatment(P<0.05).Conclusions In CORT induced mice, Yueju pill can enhance the hippocampal neurogenesis by activating the PKA-ERK-CREB signaling pathway to display antidepressant-like effect.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-09-30,修回日期:2018-10-28
基金项目:国家自然科学基金资助项目(No 81803748,81603497); 江苏省自然科学基金资助项目(No BK20161040); 江苏省高校自然科学基金资助项目(No 18KJB360009)
作者简介:马 瑶(1992-),女,硕士生,研究方向:中西医结合基础,E-mail:mayao2018@126.com;
薛文达(1984-),男,博士,助理研究员,研究方向:中医药快速抗抑郁的机制和转化医学,通讯作者,E-mail: xuewenda@njucm.edu.cn;
王 薇(1983-),女,博士,助理研究员,研究方向:产后抑郁及中药治疗的分子机制,通讯作者,E-mail: wangw@njucm.edu.cn
更新日期/Last Update: 2019-02-14