[1]刘新宇,刘春娜,李思璇,等.Urocortin对糖尿病心肌病的保护作用与Akt/GSK-3β信号通路的关系[J].中国药理学通报,2019,(07):973-977.[doi:10.3969/j.issn.1001-1978.2019.07.017]
 LIU Xin-yu,LIU Chun-na,LI Si-xuan,et al.Protective effects of urocortin on diabetic cardiomyopathy and relationship via Akt/GSK-3β signaling pathway[J].Chinese Pharmacological Bulletin,2019,(07):973-977.[doi:10.3969/j.issn.1001-1978.2019.07.017]
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Urocortin对糖尿病心肌病的保护作用与Akt/GSK-3β信号通路的关系()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年07期
页码:
973-977
栏目:
论著
出版日期:
2019-06-20

文章信息/Info

Title:
Protective effects of urocortin on diabetic cardiomyopathy and relationship via Akt/GSK-3β signaling pathway
文章编号:
1001-1978(2019)07-0973-05
作者:
刘新宇1刘春娜2李思璇1郑 晨1刘婉珠2
锦州医科大学1.附属第一医院内分泌科、2.基础医学院药理学教研室,辽宁 锦州 121001
Author(s):
LIU Xin-yu1 LIU Chun-na2 LI Si-xuan1 ZHENG Chen1 LIU Wan-zhu2
1.Dept of Endocrinology, the First Affiliated Hospital; 2.Dept of Pharmacology,Jinzhou Medical University, Jinzhou Liaoning 121001,China
关键词:
Urocortin 糖尿病心肌病 转化生长因子-β1 结缔组织生长因子 Akt 糖原合成酶激酶3β
Keywords:
urocortin diabetic cardiomyopathy transforming growth factor β1 connective tissue growth factor Akt glycogen synthase kinase 3β
分类号:
R-332; R322.11; R341; R345.57; R392.12; R587.2; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.07.017
文献标志码:
A
摘要:
目的 观察内分泌-血管活性肽Urocortin(UCN)对糖尿病心肌病(DCM)大鼠TGF-β1和CTGF的影响,研究UCN对DCM的保护作用及可能机制。方法 建立糖尿病(DM)模型,将大鼠分为5组:Control组、DCM组、UCN组、UCN+Astressin组、UCN+Triciribine组。饲养12周后处理4周,测定血糖、尿糖、尿量,以及血清TGF-β1、CTGF水平; 观察心肌细胞形态学; 测定心肌组织TGF-β1、CTGT、Akt、GSK-3β、p-Akt、p-GSK-3β的表达。结果 DM大鼠心肌形态学符合DCM改变,与Control组相比,DCM组血清与心肌组织TGF-β1、CTGF水平均增高,DCM组心肌组织p-Akt、p-GSK-3β均降低(P<0.05)。与DCM组相比,UCN组TGF-β1、CTGF水平均降低(P<0.05),Astressin和Triciribine均能阻断UCN的作用(P<0.05); UCN组p-Akt、p-GSK-3β表达增高(P<0.05),Astressin阻断UCN的作用(P<0.05)。结论 UCN对DCM的保护作用可能通过与CRH-R受体结合后,激活Akt/GSK-3β信号通路,下调炎症因子TGF-β1及CTGF的表达有关。
Abstract:
Aim To observe the effects of UCN on TGF-β1 and CTGF in rats with DCM, and to explore the protective effects of UCN on DCM and its possible signaling pathway.Methods The rats were divided into five groups: control group, DCM group, UCN group, UCN+Astressin group, and UCN+Triciribine group.After 12 weeks of feeding and 4 weeks of treatment, blood glucose, urinary sugar, urine volume and the levels of TGF-β1, CTGF in serum were determined, and the expression of TGF-β1, CTGF, Akt, GSK-3β, p-Akt and p-GSK-3β of cardiomyocytes were also determined.Results The myocardial HE staining in DM rats was consistent with DCM.The levels of TGF-β1 and CTGF in serum and myocardium of rats with DCM increased significantly, while the levels of p-Akt and p-GSK-3β in myocardium of rats with DCM decreased significantly(P<0.05).The levels of TGF-β1 and CTGF in UCN group decreased, both astressin and triciribine could inhibit the role of UCN, and the expression of p-Akt and p-GSK-3β in myocardial cells in UCN group increased significantly, then astressin could inhibit the role of UCN(P<0.01).Conclusions The protective effects of UCN on DCM might be related to the activation of Akt/GSK-3β signaling pathway after binding with CRH-R receptor, and then decreasing the expression of TGF-β1 and CTGF.

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备注/Memo

备注/Memo:
收稿日期:2019-02-16,修回日期:2019-04-26
基金项目:国家自然科学基金资助项目(No 81201037); 辽宁省重点研发计划指导计划项目(No 2018225030)
作者简介:刘新宇(1976-),男,博士,副教授,研究方向:神经内分泌学,E-mail:xxy-005@163.com;
刘婉珠(1963-),女,硕士,副教授,硕士生导师,研究方向:中药药理学,通讯作者,Tel:0416-4673465,E-mail: 43948255@qq.com
更新日期/Last Update: 2019-06-20