[1]戴 琪,黎思彤,陈健文,等.基于肠道屏障功能研究乌司他丁对大鼠术后肠梗阻的影响[J].中国药理学通报,2019,(08):1066-1072.[doi:10.3969/j.issn.1001-1978.2019.08.008]
 DAI Qi,LI Si-tong,CHEN Jian-wen,et al.Effects of ulinastatin on rat postoperative ileus and intestinal barrier function[J].Chinese Pharmacological Bulletin,2019,(08):1066-1072.[doi:10.3969/j.issn.1001-1978.2019.08.008]
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基于肠道屏障功能研究乌司他丁对大鼠术后肠梗阻的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1066-1072
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Effects of ulinastatin on rat postoperative ileus and intestinal barrier function
文章编号:
1001-1978(2019)08-1066-07
作者:
戴 琪1黎思彤1陈健文1谭 飞2郑志华1张志军2刘培庆1李 民1
1.中山大学药学院,新药成药性评估与评价国家地方联合工程实验室,广东 广州 510006; 2.广东天普生化医药股份有限公司,广东 广州 510520
Author(s):
DAI Qi1 LI Si-tong1 CHEN Jian-wen1 TAN Fei2ZHENG Zhi-hua1 ZHANG Zhi-jun2 LIU Pei-qing1 LI Min1
1.School of Pharmaceutical Sciences, National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Sun Yat-Sen University, Guangzhou 510006, China; 2.Techpool Bio-pharmaceutical Co., Ltd, Guangzhou 510520, China
关键词:
术后肠梗阻 乌司他丁 肠道屏障 杯状细胞 抗炎 肠黏膜
Keywords:
postoperative ileus ulinastatin intestinal barrier function goblet cells anti-inflammatory intestinal mucosa
分类号:
R-332; R322.45; R333.3; R364.5; R574.2; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.08.008
文献标志码:
A
摘要:
目的 研究乌司他丁(ulinastatin, UTI)对术后肠梗阻(postoperative ileus, POI)模型SD大鼠的作用,以及对肠道屏障功能的影响。方法 在SD大鼠腹腔手术前、手术后及围术期分3种剂量给予UTI。术后48 h伊文氏蓝经口灌胃,30 min后解剖检测胃肠推进率; 用回肠末端HE和AB/PAS染色切片,观察小肠绒毛和统计杯状细胞数量; D-乳酸和内毒素试剂盒评估肠通透性; 提取肠组织cDNA进行qPCR,检测炎症因子及肠道屏障相关的mRNA水平。结果 POI模型组SD大鼠胃肠推进率下降,小肠绒毛结构严重破坏,D-乳酸和内毒素水平及炎症因子mRNA水平升高,隐窝处杯状细胞数量增高,黏蛋白2(mucin 2,MUC2)、黏蛋白3(mucin 3, MUC3)mRNA水平上升,人防御素5(human defensing 5, HD5)mRNA水平下降。UTI中剂量预处理能明显逆转上述情况。结论 UTI中剂量预处理有效降低POI大鼠肠道炎症水平,恢复部分肠道屏障功能,从而预防和治疗POI造成的胃肠推进率的下降。
Abstract:
Aim To study the effect of ulinastatin(UTI)on postoperative ileus(POI)and the intestinal barrier function in SD rats.Methods UTI was injected in three doses before, after and during abdominal surgery in SD rats.Evans blue was given by gavage 48 hours after the surgery and gastrointestinal propulsion rate was measured 30 minutes later.The end of ileum was collected for HE staining and AB/PAS staining to make tissue sections.The morphology of the intestinal villi and the number of goblet cells were observed under a light microscope.D-lactate and endotoxin kits were used to evaluate intestinal permeability.cDNA was extracted from the intestinal tissues to detect the levels of inflammatory factors and intestinal barrier-related genes by qPCR.Results In POI model group, the gastrointestinal propulsion rate decreased, and villi structure of small intestine was severely damaged; the levels of D-lactate, endotoxin and inflammatory factor mRNA increased; the number of goblet cells in crypt increased; the levels of MUC2 and MUC3 mRNA increased; the level of HD5 mRNA decreased.Pretreatment with medium dose UTI could significantly reverse the above situation.Conclusions Pretreatment with medium dose UTI can effectively reduce the intestinal inflammation and restore partially the intestinal barrier function in POI rats, thus preventing and treating the decrease of gastrointestinal propulsion rate caused by POI.

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备注/Memo

备注/Memo:
收稿日期:2019-04-20,修回日期:2019-05-19
基金项目:广东省自然科学基金(No 2016A030313335); 广州市科技计划项目产学研协同创新重大专项(No 201604020121); 广东省应用型科技研发专项(No 2015B020232009); 广东省科技厅公益研究与能力建设专项(No 2014B020210003)
作者简介:戴 琪(1994-),女,硕士生,研究方向:药物评价,E-mail: dqdq0908@126.com;
李 民(1976-),男,博士,副教授,博士生导师,研究方向:分子药理学、细胞自噬,通讯作者,E-mail: limin65@mail.sysu.edu.cn;
刘培庆(1967-),男,博士,教授,博士生导师,研究方向:心血管药理学、成药性评价,通讯作者,E-mail: liupq@mail.sysu.edu.cn
更新日期/Last Update: 2019-08-14