[1]胡 露,李洪忠,万敬员.DERL1对人乳腺癌细胞ZR-75-1迁移和侵袭的影响[J].中国药理学通报,2019,(08):1079-1083.[doi:10.3969/j.issn.1001-1978.2019.08.010]
 HU Lu,LI Hong-zhong,WAN Jing-yuan.Effect of DERL1 on migration and invasion of human breast cancer cells ZR-75-1[J].Chinese Pharmacological Bulletin,2019,(08):1079-1083.[doi:10.3969/j.issn.1001-1978.2019.08.010]
点击复制

DERL1对人乳腺癌细胞ZR-75-1迁移和侵袭的影响()
分享到:

《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1079-1083
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Effect of DERL1 on migration and invasion of human breast cancer cells ZR-75-1
文章编号:
1001-1978(2019)08-1079-05
作者:
胡 露1李洪忠2万敬员1
1.重庆医科大学药学院,重庆 400016; 2.重庆医科大学附属第一医院分子肿瘤学与表观遗传学实验室,重庆 400016
Author(s):
HU Lu1 LI Hong-zhong2 WAN Jing-yuan1
1.College of Pharmacy, Chongqing Medical University, Chongqing 400016, China; 2.Molecular Oncology and Epigenetics Lab, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
关键词:
DERL1 乳腺癌 迁移 侵袭 上皮间质转化 E-cadherin
Keywords:
DERL1 breast cancer migration invasion epithelial mesenchymal transition E-cadherin
分类号:
R329.24; R394.2; R73-37; R737.902.2; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.08.010
文献标志码:
A
摘要:
目的 探讨DERL1(Der1-like domain family,member 1)对人乳腺癌细胞ZR-75-1迁移和侵袭的影响及其相关机制。方法 将DERL1过表达或干扰质粒转入人乳腺癌ZR-75-1细胞中,实时荧光定量PCR检测DERL1 mRNA的表达; 划痕愈合实验和Transwell实验分别检测DERL1对ZR-75-1迁移和侵袭能力的影响; 实时荧光定量PCR和细胞免疫荧光分别检测ZR-75-1中E-cadherin mRNA和蛋白的表达情况。结果 DERL1过表达质粒转染48 h后,细胞中DERL1表达水平较对照组明显上升(P<0.01),同时细胞的迁移和侵袭能力均明显增强(P<0.05),细胞形态由鹅卵石形上皮样转化为纺锤形间质样,且细胞中E-cadherin mRNA和蛋白水平明显降低(P<0.05); DERL1干扰组细胞转染48 h后,与对照组相比,DERL1表达水平明显降低(P<0.01),同时细胞的迁移和侵袭能力明显减弱(P<0.05),细胞形态由纺锤形间质样转变回鹅卵石形上皮样,且细胞中E-cadherin的mRNA和蛋白表达量明显升高(P<0.05)。结论 DERL1可促进人乳腺癌细胞ZR-75-1的迁移和侵袭,可能与其下调E-cadherin水平,促进ZR-75-1的上皮间质转化有关。
Abstract:
Aim To investigate the effect of DERL1 on migration and invasion of human breast cancer cells ZR-75-1 and its potential mechanism.Methods Plasmid of DERL1 overexpression or DERL1 interference was used to explore the function of DERLI in breast cancer cells ZR-75-1.The migration and invasion of ZR-75-1 cells were analyzed using wound healing assays and Transwell assays.In addition, the expression of E-cadherin mRNA and protein in ZR-75-1 cells was detected by Real-time quantitative PCR and immunofluorescence.Results After plasmid of DERL1 overexpression transfection for 48 h, the expression of DERL1 in ZR-75-1 cells significantly increased compared with that of control group(P<0.01), and the migration and invasion of cells were significantly up-regulated(P<0.05), cell morphology was transformed from cobblestone-like epithelial to spindle-shaped mesenchymal, and the expression of E-cadherin mRNA and protein significantly decreased(P<0.05).After plasmid of DERL1 interference transfection for 48 h, compared with control group, the expression of DERL1 in ZR-75-1 cells was significantly down-regulated(P<0.01), DERL1 knockdown inhibited the migration and invasion of ZR-75-1 cells(P<0.05), cell morphology changed from spindle-shaped mesenchymal to cobblestone-like epithelial, and the expression of E-cadherin mRNA and protein was significantly up-regulated(P<0.05).Conclusions DERL1 can promote the migration and invasion of human breast cancer cells ZR-75-1, which may be related to its inhibition of E-cadherin expression to promotion of epithelial mesenchymal transition.

参考文献/References:

[1] Siegel R L, Miller K D.Jemal A.Cancer statistics, 2018[J].CA Cancer J Clin, 2018, 68(1): 7-30.
[2] 孙银辉.Derlin-1在RLE-6TN细胞内质网应激诱导性凋亡中的作用研究[D].衡阳:南华大学,2015.
[2] Sun Y H.Derlin-1 plays a role in endoplasmic reticulum stress-induced apoptosis in RLE-6TN cells[D].Hengyang: Nanhua University, 2015.
[3] Mao M, Zhang J, Jiang J, et al.Overexpression of Derlin-1 is associated with poor prognosis in patients with non-small cell lung cancer[J].Ann Clin Lab Sci, 2018, 48(1): 29-34.
[4] Tan X, He X, Jiang Z, et al.Derlin-1 is overexpressed in human colon cancer and promotes cancer cell proliferation[J].Mol Cell Biochem, 2015, 408(1-2): 205-13.
[5] Wang J, Hua H, Ran Y, et al.Derlin-1 is overexpressed in human breast carcinoma and protects cancer cells from endoplasmic reticulum stress-induced apoptosis[J].Breast Cancer Res, 2008, 10(1):R7.
[6] Ran Y, Hu H, Hu D, et al.Derlin-1 is overexpressed on the tumor cell surface and enables antibody-mediated tumor targeting therapy[J].Clin Cancer Res, 2008,14(20):6538-45.
[7] 彭 艳, 胡瑞成, 戴爱国.Derlin-1的研究进展[J].中国老年学杂志, 2013, 33(1): 215-7.
[7] Peng Y, Hu R C, Dai A G.Research progress of Derlin-1[J].Chin J Gerontol, 2013, 33(1): 215-7.
[8] Pi L,Zhu G,She L, et al.Elevated expression of Derlin-1 associates with unfavorable survival time of squamous cell carcinoma of the head and neck and promotes its malignance[J].J Cancer, 2017, 8(12):2336-45.
[9] Wu Z, Wang C, Zhang Z, et al.High expression of Derlin-1 is associated with the malignancy of bladder cancer in a Chinese Han population[J].PLoS One, 2016, 11(12): e0168351.
[10] Daugaard I, Sanders K J, Idica A, et al.miR-151a induces partial EMT by regulating E-cadherin in NSCLC cells[J].Oncogenesis, 2017,6(7):e366.
[11] 李汉清,可 燕.上皮间质转化的机制研究进展[J].中国药理学通报, 2017,33(10):1342-4.
[11] Li H Q, Ke Y.Mechanism of epithelial-mesenchymal transition[J].Chin Pharmacol Bull, 2017,33(10):1342-4.
[12] Yu H, Shen Y, Hong J, et al.The contribution of TGF-β in epithelial-mesenchymal transition(EMT): down-regulation of E-cadherin via snail[J].Neoplasma, 2015, 62(1):1-15.
[13] 黄礼义, 林海丹, 虞乐华, 白定群.焦脱镁叶绿酸甲酯介导光动力抑制人乳腺癌细胞MCF-7迁移[J].中国药理学通报, 2019, 35(1):24-9.
[13] Huang L Y, Lin H D, Yu L H, Bai D Q.Pyropheophorbide-a methyl ester-mediated photodynamic therapy inhibits migration of human breast cancer MCF-7 cells[J].Chin Pharmacol Bull, 2019, 35(1):24-9.
[14] Markiewicz A, Welnicka-Jaski'ewicz M, Seroczyńska B, et al.Epithelial-mesenchymal transition markers in lymph node metastases and primary breast tumors-relation to dissemination and proliferation[J].Am J Transl Res, 2014, 6(6):793-808.

相似文献/References:

[1]林 姝,焦旭阳,赵 琳,等.miR-181a对乳腺癌耐药蛋白表达调控作用的研究[J].中国药理学通报,2014,(08):1073.
 LIN Shu,JIAO Xu-yang,ZHAO Lin,et al.Regulatory effect of miR-181a on breast cancer resistance protein[J].Chinese Pharmacological Bulletin,2014,(08):1073.
[2]杨 烨,颜晓静,毕 蕾,等.正交设计优选丹参-人参活性组分抗乳腺癌有效配伍[J].中国药理学通报,2014,(11):1605.
 YANG Ye,YAN Xiao-jing,BI Lei,et al.Optimization of effective component formula from active ingredients of Salvia Miltiorrhiza and Panax Ginseng through orthogonal design method to resist breast cancer[J].Chinese Pharmacological Bulletin,2014,(08):1605.
[3]王佳艺,何俊劲,郝静超,等.多肽AP25与多西他赛联合用药对人乳腺癌裸鼠移植瘤的抑制活性研究[J].中国药理学通报,2015,(09):1233.[doi:10.3969/j.issn.1001-1978.2015.09.011]
 WANG Jia-yi,HE Jun-jin,HAO Jing-chao,et al.Combination of polypeptide AP25 and docetaxel in the treatment of breast cancer[J].Chinese Pharmacological Bulletin,2015,(08):1233.[doi:10.3969/j.issn.1001-1978.2015.09.011]
[4]陈锡强,韩利文,王希敏,等.人乳腺癌斑马鱼移植瘤模型建立[J].中国药理学通报,2016,(01):128.[doi:10.3969/j.issn.1001-1978.2016.01.027]
 CHEN Xi-qiang,HAN Li-wen,WANG Xi-min,et al.Model establishment of xenotransplantation of human breast cancer in zebrafish embryos[J].Chinese Pharmacological Bulletin,2016,(08):128.[doi:10.3969/j.issn.1001-1978.2016.01.027]
[5]韩 翰,王 敏.SAHA和TRAIL联合使用对乳腺癌雌激素受体阳性细胞MCF-7生长的影响[J].中国药理学通报,2016,(02):223.[doi:10.3969/j.issn.1001-1978.2016.02.015]
 HAN Han,WANG Min.Effects of combination treatment with SAHA and TRAIL on ER positive breast cancer cell MCF-7[J].Chinese Pharmacological Bulletin,2016,(08):223.[doi:10.3969/j.issn.1001-1978.2016.02.015]
[6]冯秀艳,韩 翰,周伟强.SAHA在Leptin诱导的乳腺癌MCF-7细胞增殖过程中的调控作用[J].中国药理学通报,2016,(04):503.[doi:10.3969/j.issn.1001-1978.2016.04.013]
 FENG Xiu-yan,HAN Han,ZHOU Wei-qiang.Regulation of SAHA on cell proliferation induced by leptin in breast cancer cell line MCF-7[J].Chinese Pharmacological Bulletin,2016,(08):503.[doi:10.3969/j.issn.1001-1978.2016.04.013]
[7]路玉盼,董宪喆,冯 霞,等.血根碱抑制人乳腺癌细胞MCF-7增殖的机制研究[J].中国药理学通报,2016,(06):858.[doi:10.3969/j.issn.1001-1978.2016.06.023]
 LU Yu-pan,DONG Xian-zhe,FENG Xia,et al.Sanguinarine inhibits cell proliferation in MCF-7 human mammary adenocarcinoma cells[J].Chinese Pharmacological Bulletin,2016,(08):858.[doi:10.3969/j.issn.1001-1978.2016.06.023]
[8]袁立明,马 楠,曹交欢,等.顺铂加重乳腺癌MCF-7细胞DNA损伤促凋亡的研究[J].中国药理学通报,2017,(03):334.[doi:10.3969/j.issn.1001-1978.2017.03.009]
 YUAN Li-ming,MA Nan,CAO Jiao-huan,et al.Study of cisplatin aggravating DNA damage and causing a high apoptosis rate on breast cancer MCF-7 cells[J].Chinese Pharmacological Bulletin,2017,(08):334.[doi:10.3969/j.issn.1001-1978.2017.03.009]
[9]孙志亮,李洪利,尹崇高.TGF-β1通过F-actin聚合促进乳腺癌细胞MCF-7发生上皮-间质转化[J].中国药理学通报,2017,(05):735.[doi:10.3969/j.issn.1001-1978.2017.05.028]
 SUN Zhi-liang,LI Hong-li,YIN Chong-gao.TGF-β1 promotes epithelial-mesenchymal transition in breast cancer cell line MCF-7 through F-actin polymerization[J].Chinese Pharmacological Bulletin,2017,(08):735.[doi:10.3969/j.issn.1001-1978.2017.05.028]
[10]徐新伟,王照岩,王瑞鸽,等.Raptor通过上皮-间质转化促进乳腺癌的侵袭与转移[J].中国药理学通报,2017,(08):1091.[doi:10.3969/j.issn.1001-1978.2017.08.011]
 XU Xin-wei,WANG Zhao-yan,WANG Rui-ge,et al.Raptor induces migration and invasion of breast cancer through epithelial-mesenchymal transition[J].Chinese Pharmacological Bulletin,2017,(08):1091.[doi:10.3969/j.issn.1001-1978.2017.08.011]

备注/Memo

备注/Memo:
收稿日期:2019-05-10,修回日期:2019-06-18
基金项目:国家自然科学基金资助项目(No 81472475)
作者简介:胡 露(1994-),女,硕士生,研究方向:炎症与免疫药理学,E-mail:hulu6220@163.com;
万敬员(1972-),男,博士,教授,硕士生导师,研究方向:炎症与免疫药理学,通讯作者,E-mail:jywan@cqmu.edu.cn
更新日期/Last Update: 2019-08-14