[1]房志锐,陈 璐,李春晓,等.芍药苷促进糖尿病创面愈合[J].中国药理学通报,2019,(08):1084-1091.[doi:10.3969/j.issn.1001-1978.2019.08.011]
 FANG Zhi-rui,CHEN Lu,LI Chun-xiao,et al.Paeoniflorin accelerates diabetic wound healing[J].Chinese Pharmacological Bulletin,2019,(08):1084-1091.[doi:10.3969/j.issn.1001-1978.2019.08.011]
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芍药苷促进糖尿病创面愈合()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1084-1091
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Paeoniflorin accelerates diabetic wound healing
文章编号:
1001-1978(2019)08-1084-08
作者:
房志锐1陈 璐1李春晓1宋 敏1张璐莎1Joel Wake Coffie1张丽媛1马璐璐1王 虹2
1.方剂学教育部重点实验室,天津市中药药理学重点实验室,天津中医药大学中医药研究院,天津 301617; 2.天津中医药大学中西医结合学院,天津 301617
Author(s):
FANG Zhi-rui CHEN Lu LI Chun-xiao SONG Min ZHANG Lu-sha Joel Wake Coffie ZHANG Li-yuan MA Lu-lu WANG Hong
1.Key Lab of Prescription Medicine of Ministry of Education, Tianjin University of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin State Key Lab of Traditional Chinese Medicine Pharmacology, Tianjin 301617, China; 2.School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
关键词:
芍药苷 糖尿病创面愈合 血管新生 细胞外基质 内皮细胞 成纤维细胞
Keywords:
paeoniflorin diabetic wound healing angiogenesis extracellular matrix endothelial cells fibroblasts
分类号:
R-332; R284.1; R329.24; R364.3; R587.1; R641
DOI:
10.3969/j.issn.1001-1978.2019.08.011
文献标志码:
A
摘要:
目的 研究芍药苷(paeoniflorin,PA)对糖尿病创面愈合作用及作用机制。方法 采用糖尿病小鼠全层皮肤切除夹板模型,HE染色及Masson染色观察创面组织形态改变; 免疫荧光法检测血管新生。PA干预人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)和小鼠成纤维细胞(fibroblast,FB),MTS、BrdU和划痕实验检测细胞增殖和迁移能力; Matrigel实验检测HUVECs成管能力,qPCR检测FB中Collagen Ⅲ、Fibronectin、α-SMA mRNA的变化; 免疫荧光法观察α-SMA在FB中的表达部位及表达量的变化。结果 与db/db模型组相比,PA组创面肉芽组织,胶原形成和新生毛细血管密度明显增加(P<0.01)。PA对HUVECs增殖无影响,可明显促进HUVECs迁移和成管的能力(P<0.05, P<0.01)。PA可明显增加FB增殖和迁移的能力,明显增加Collagen Ⅲ、α-SMA mRNA的表达(P<0.05, P<0.01),对Fibronectin mRNA的表达无影响,同时可增加α-SMA的荧光强度。结论 芍药苷可能通过促进细胞外基质生成和血管新生,促进糖尿病创面愈合。
Abstract:
Aim To observe the effectof paeoniflorin(PA)on diabetic wound healing and its mechanisms.Methods A full-thickness skin excision splint model in diabetic mice was used to study the effects of PA on diabetic wound healing and the underlying mechanism.HE staining and Masson staining were used to observe the changes of granulation tissue and collagen in wound tissues.Immunofluorescence technique was employed to detect angiogenesis in wound tissues of diabetic mice.To detect the effects of PA, human umbilical vein endothelial cells(HUVECs)and mouse fibroblasts were cultured in vitro.MTS, BrdU and scratch assays were used to detect the effects of PA on proliferation and migration.Tube formation assays were used to detect the effects of PA on the tube formation of HUVECs.qPCR was applied to assess the expression of collagen Ⅲ, fibronectin and α-SMA gene.Immunofluorescence was used to detect the expression of α-SMA.Results Compared with db/db model group, the formation rate of granulation tissues and collagen and the capillary density around the wound of db/db model group treated with PA was significantly higher than that of db/db model group(P.MTS, BrdU and scratch assays were used to detect the effects of PA on proliferation and migration.Tube formation assays were used to detect the effects of PA on the tube formation of HUVECs.qPCR was applied to assess the expression of collagen Ⅲ, fibronectin and α-SMA gene.Immunofluorescence was used to detect the expression of α-SMA.Results Compared with db/db model group, the formation rate of granulation tissues and collagen and the capillary density around the wound of db/db model group treated with PA was significantly higher than that of db/db model group(;0.01).PA had no significant effect on endothelial cell proliferation, but it could markedly promote endothelial cell migration and tube formation.PA could significantly up-regulate the migration, proliferation, secretion and differentiation of fibroblasts.Conclusions PA can significantly promote diabetic wound healing, which may be related to accelerating the generation of extracellular matrix and promoting angiogenesis.

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备注/Memo

备注/Memo:
收稿日期:2019-04-11,修回日期:2019-05-16
基金项目:国家国际科技合作专项(No 2015DFA30430); 天津市自然科学基金重点项目(No 16JCZDJC36300); 国家自然科学基金青年基金项目(No 81603329)
作者简介:房志锐(1994-),女,硕士生,研究方向:中药心血管药理学,E-mail:fangzhiruiahmu@126.com;
王 虹(1974-),女,博士,研究员,研究方向:中药心血管药理学,通讯作者,E-mail:wanghongsys@126.com
更新日期/Last Update: 2019-08-14