[1]郭 珮,李 静,冉建华,等.注射用核糖核酸Ⅱ通过活性氧介导的PI3K/Akt信号通路诱导人白血病细胞KG1a凋亡[J].中国药理学通报,2019,(08):1092-1098.[doi:10.3969/j.issn.1001-1978.2019.08.012]
 GUO Pei,LI Jing,RAN Jian-hua,et al.Ribonucleic acid Ⅱ induces apoptosis of human leukemia KG1a cells through PI3K/Akt activation mediated by reactive oxygen[J].Chinese Pharmacological Bulletin,2019,(08):1092-1098.[doi:10.3969/j.issn.1001-1978.2019.08.012]
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注射用核糖核酸Ⅱ通过活性氧介导的PI3K/Akt信号通路诱导人白血病细胞KG1a凋亡()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1092-1098
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Ribonucleic acid Ⅱ induces apoptosis of human leukemia KG1a cells through PI3K/Akt activation mediated by reactive oxygen
文章编号:
1001-1978(2019)08-1092-07
作者:
郭 珮12李 静1冉建华3陈地龙14何 菲3吕晓婷1石雪萍1李海星1
1.重庆医科大学基础医学院组织细胞工程与干细胞研究室,重庆 400016; 2.重庆市第六人民医院/重庆市职业病防治院药学部,重庆 400060; 3.重庆医科大学基础医学院解剖学教研室,重庆 400016; 4.重庆三峡医药高等专科学校,重庆 404120
Author(s):
GUO Pei12 LI Jing1 RAN Jian-hua3 CHEN Di-long14 HE Fei3 LYU Xiao-ting1 SHI Xue-ping1 LI Hai-xing1
1.Lab of Stem Cells and Tissue Engineering, College of Basic Medicine, Chongqing Medical University,Chongqing 400016, China; 2.Dept of Pharmacy, Occupational Disease Prevention Hospital, Chongqing 400060,China; 3.Dept of Anatomy, College of Basic Medicines, Chongqing Medical University, Chongqing 400016,China; 4.Chongqing Three Gorges Medical College, Chongqing 404120, China
关键词:
注射用核糖核酸Ⅱ 白血病 KG1a PI3K/Akt 活性氧 凋亡
Keywords:
ribonucleic acid II leukemia KG1a PI3K/Akt ROS apoptosis
分类号:
R329.25; R329.28; R342.2; R394.2; R733.702.2; R977; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.08.012
文献标志码:
A
摘要:
目的 研究注射用核糖核酸Ⅱ诱导人急性骨髓白血病细胞系KG1a细胞凋亡与PI3K/Akt信号通路和活性氧(ROS)的关系。方法 流式细胞术(FCM)检测细胞周期和细胞内的ROS水平; 免疫印迹法(Western blot)检测PI3K/Akt信号通路相关蛋白p-PI3K、p-Akt、p-MDM2、p21和周期相关蛋白cyclin D1、CDK4的表达; 加入抗氧化剂N-乙酰半胱氨酸(NAC)后,检测p-PI3K、p-Akt和p53蛋白表达变化。结果 FCM结果显示,注射用核糖核酸Ⅱ可将KG1a细胞阻滞在G0/G1期,且ROS介导了细胞周期的阻滞; FCM检测ROS结果显示,KG1a细胞内ROS水平均随药物浓度增加而升高; Western blot结果显示,注射用核糖核酸Ⅱ下调p-PI3K、p-Akt、p-MDM2和cyclin D1、CDK4的表达,上调p21的表达; 与仅用注射用核糖核酸Ⅱ相比,经NAC预处理后再使用注射用核糖核酸Ⅱ,明显上调了磷酸化PI3K和Akt的表达,下调了p53的表达。结论 注射用核糖核酸Ⅱ通过ROS介导的PI3K/Akt信号通路,诱导人急性骨髓白血病细胞系KG1a细胞凋亡。
Abstract:
Aim To investigate the correlation between ribonucleic acid Ⅱ on human leukemia cell lines KG1a and PI3K/Akt signaling pathway by reactive oxygen.Methods The cell cycle and the ROS level of ribonucleic acid Ⅱ in human leukemia cell lines were determined by flow cytometry.The levels of p-PI3K, p-Akt, p-MDM2, p21, cyclin D1 and CDK4 were detected by Western blot.The expression levels of protein p-PI3K, p-Akt and p53 after treated with ribonucleic acid Ⅱ and NAC were also detected by Western blot.Results The results of FCM revealed that the cell cycle was arrested at G0/G1 phase and ROS pre-vented the arrest of cell cycle.The results also showed that ROS level increased with the concentration of ribonucleic acid Ⅱ in KG1a cells.Western blot results showed after treated with ribonucleic acid Ⅱ, the expression levels of p-PI3K, p-Akt, p-MDM2, cyclin D1 and CDK4 decreased, while p21 increased in KG1a cells.After treated with ribonucleic acid Ⅱ and NAC, the expression levels of p-PI3K and p-Akt increased, while p53 decreased.Conclusion Ribonucleic acid Ⅱ can induce the apoptosis of leukemia KG1a cells through ROS-mediated PI3K/Akt signaling pathway.

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备注/Memo

备注/Memo:
收稿日期:2019-03-18,修回日期:2019-04-15
基金项目:重庆市科技计划项目(No cstc2015jcyjA0036)
作者简介:郭 珮(1991-),女,硕士生,研究方向:白血病基础与临床,E-mail:930029089@qq.com;
冉建华(1974-),女,博士,教授,研究方向:尿素通道蛋白生理功能及药物开发,通讯作者,E-mail:1315038024@qq.com
李 静(1973-),女,博士,副教授,研究方向:中药药理学,通讯作者,E-mail:lijingyangyang@126.com
更新日期/Last Update: 2019-08-14