[1]王 石,周大臣,崔 笑,等.七叶皂苷钠作用EIF4A1抑制肝癌细胞增殖的研究[J].中国药理学通报,2019,(08):1120-1125.[doi:10.3969/j.issn.1001-1978.2019.08.017]
 WANG Shi,ZHOU Da-chen,CUI Xiao,et al.Study on sodium aescinate inhibiting hepatocellular carcinoma by EIF4A1[J].Chinese Pharmacological Bulletin,2019,(08):1120-1125.[doi:10.3969/j.issn.1001-1978.2019.08.017]
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七叶皂苷钠作用EIF4A1抑制肝癌细胞增殖的研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1120-1125
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Study on sodium aescinate inhibiting hepatocellular carcinoma by EIF4A1
文章编号:
1001-1978(2019)08-1120-06
作者:
王 石周大臣崔 笑张 彬侯 辉耿小平
安徽医科大学第二附属医院肝胆外科,安徽 合肥 230601
Author(s):
WANG Shi ZHOU Da-chen CUI Xiao ZHANG Bin HOU Hui GENG Xiao-ping
Dept of Hepatobiliary Surgery, the Second Hospital of Anhui Medical University, Hefei 230601, China
关键词:
七叶皂苷钠 EIF4A1 肝细胞性肝癌 免疫组化 生存分析 HepG2
Keywords:
sodium aescinate EIF4A1 hepatocellular carcinoma immunohistochemical staining subsistence analysis HepG2
分类号:
R284.1; R322.47; R329.24; R394.2; R735.7
DOI:
10.3969/j.issn.1001-1978.2019.08.017
文献标志码:
A
摘要:
目的 研究EIF4A1的表达与肝细胞性肝癌(hepatocellular carcinoma, HCC)的相关性,以及七叶皂苷钠抑制肝癌细胞系增殖的潜在机制。方法 应用免疫组化检测80例HCC患者的肿瘤标本中EIF4A1的表达水平,并结合临床指标和随访信息分析其相关性; Annexin V-FITC/PI双染标记法检测七叶皂苷钠对肝癌细胞HepG2及人肝细胞L02两种细胞系凋亡的影响; Transwell迁移实验检测七叶皂苷钠对两种细胞系迁移能力的影响; Western blot、qPCR、细胞免疫荧光检测七叶皂苷钠处理两种细胞系后EIF4A1的表达变化。结果 肝癌组织中EIF4A1表达量明显增高,且EIF4A1的表达与患者的肿瘤分化程度、肿瘤直径及生存期存在相关性。七叶皂苷钠(40 μmol·L-1)可明显促进肝癌细胞系凋亡,并抑制其迁移能力,但对于正常肝细胞系无影响。七叶皂苷钠抑制肝癌细胞系生长增殖的同时,下调肝癌细胞系EIF4A1的表达。结论 EIF4A1的表达与HCC的发展呈相关性,七叶皂苷钠可能通过影响EIF4A1的表达,抑制肝癌细胞系的生长增殖。
Abstract:
Aim To investigate the correlation between the expression of EIF4A1 and hepatocellular carcinoma(HCC)and the potential mechanism of sodium aescinate inhibiting the proliferation of hepatoma cell lines.Methods Immunohistochemistry was used to detect the expression level of EIF4A1 in tumor specimens of 80 patients with HCC.The results combined with clinical indicators and follow-up information were used to analyze their relevance.Annexin V-FITC/PI double staining method was employed to detect the effects of sodium aescinate on apoptosis of HepG2 and human L02 cell lines.Transwell migration assay was used to detect the effect of sodium aescinate on the migration of two cell lines.Western blot, qPCR and immunofluorescence were used to detect the expression changes of EIF4A1 of two cell lines after sodium aescinate treatment.Results The expression of EIF4A1 significantly increased in HCC tissues, and the expression of EIF4A1 was correlated with tumor differentiation, tumor diameter and survival time.Sodium aescinate(40 μmol·L-1)could significantly promote the apoptosis of liver cancer cell line and inhibit its migration ability, but had no effect on normal liver cell line.Sodium aescinate inhibited the growth and proliferation of hepatoma cell line while down-regulated the expression of hepatoma cell line EIF4A1.Conclusions EIF4A1 is associated with the development of HCC, and sodium aescinate can inhibit hepatoma cell line via affecting the expression of EIF4A1.

参考文献/References:

[1] 中华人民共和国卫生和计划生育委员会医政医管局.原发性肝癌诊疗规范(2017年版)[J].中华消化外科杂志, 2017,16(7):705-20.
[1] Health and Family Planning Commission of the People's Republic of China.Primary liver cancer diagnosis and treatment specification(2017 edition)[J].Chin J Digestive Surgery, 2017,16(7):705-20.
[2] Cui S X, Shi W N, Song Z Y, et al.Des-gamma-carboxy prothrombin antagonizes the effects of Sorafenib on human hepatocellular carcinoma through activation of the Raf/MEK/ERK and PI3K/Akt/mTOR signaling pathways[J].Oncotarget, 2016,7(24):36767-82.
[3] Yang S, Luo C, Gu Q, et al.Activating JAK1 mutation may predict the sensitivity of JAK-STAT inhibition in hepatocellular carcinoma[J].Oncotarget, 2016,7(5):5461-9.
[4] Wang R, Sun Q, Wang P, et al.Notch and Wnt/beta-catenin signaling pathway play important roles in activating liver cancer stem cells[J].Oncotarget, 2016,7(5):5754-68.
[5] Le Quesne J P, Spriggs K A, Bushell M.Dysregulation of protein synthesis and disease[J].J Pathol, 2010,220(2):140-51.
[6] Boussemart L, Malka-Mahieu H, Girault I, et al.eIF4F is a nexus of resistance to anti-BRAF and anti-MEK cancer therapies[J].Nature, 2014,513(7516):105-9.
[7] Svitkin Y V, Pause A, Haghighat A, et al.The requirement for eukaryotic initiation factor 4A(elF4A)in translation is in direct proportion to the degree of mRNA 5' secondary structure[J].RNA, 2001,7(3):382-94.
[8] Ji P, Diederichs S, Wang W, et al.MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer[J].Oncogene,2003,22(39):8031-41.
[9] Liang S, Zhou Y, Chen Y.Decreased expression of EIF4A1 after preoperative brachytherapy predicts better tumor-specific survival in cervical cancer[J].Int J Gynecol Cancer, 2014,24(5):908-15.
[10] Modelska A, Turro E, Russell R, et al.The malignant phenotype in breast cancer is driven by eIF4A1-mediated changes in the translational landscape[J].Cell Death Dis, 2015,6:e1603.
[11] 李 珊, 马玲云, 李向日, 等.中药娑罗子的现代研究进展[J].亚太传统医药, 2012,08(8):178-81.
[11] Li S, Ma L Y, Li X R, et al.Advances in modern research on Chinese medicine Semen Aesculi[J].Asia-Pacific Tradit Med, 2012,08(8):178-81.
[12] 李未祥, 周大臣, 王 石, 等.七叶皂苷钠通过CARMA3/NF-κB信号通路抑制肝癌细胞的增殖[J].中国药理学通报, 2018,34(9):1243-8.
[12] Li W X, Zhou D C, Wang S, et al.Sodium aescinate inhibits the proliferation of hepatocellular carcinoma through the CARMA3/NF-κB signaling pathway[J].Chin Pharmacol Bull, 2018,34(9):1243-8.
[13] 齐世美,吕 俊,孟 宇, 等.七叶皂苷钠通过抑制AKT, ERK上游信号SRC活性诱导人乳腺癌MCF-7细胞凋亡[J].中国中药杂志, 2015,40(16):3267-72.
[13] Qi S M, Lyu J, Meng Y, et al.Effect of sodium aescinate in inducing human breast cancer MCF-7 cells apoptosis by inhibiting AKT, ERK and upstream signal SRC activity[J].Chin J Chin Mater Med, 2015,40(16):3267-72.
[14] Feoktistova K, Tuvshintogs E, Do A, Fraser C S.Human eIF4E promotes mRNA restructuring by stimulating eIF4A helicase activity[J].Proc Natl Acad Sci USA, 2013,110(33):13339-44.

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备注/Memo

备注/Memo:
收稿日期:2019-04-17,修回日期:2019-05-21
基金项目:安徽省高等学校自然科学基金资助项目(No KJ2017A825); 安徽医科大学校研究基金(No 2017xkj034)
作者简介:王 石(1990-),男,硕士生,研究方向:肝胆外科,E-mail:528418989@qq.com;
耿小平(1956-),男,主任医师,教授,博士生导师,研究方向:肝胆胰外科,通讯作者,E-mail:xp_geng@163.com
更新日期/Last Update: 2019-08-14