[1]张紫文,李晨晨,潘瑞乐,等.基于蛋白质组学探讨粗壮女贞总苷对高脂血症金黄地鼠的调脂作用机制[J].中国药理学通报,2019,(08):1126-1133.[doi:10.3969/j.issn.1001-1978.2019.08.018]
 ZHANG Zi-wen,LI Chen-chen,PAN Rui-le,et al.Proteomic-based studies on hypolipidemic mechanism of total phenylpropanoid glycosides from Ligustrum robustum(Roxb.) Blume in hyperlipidemic hamsters[J].Chinese Pharmacological Bulletin,2019,(08):1126-1133.[doi:10.3969/j.issn.1001-1978.2019.08.018]
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基于蛋白质组学探讨粗壮女贞总苷对高脂血症金黄地鼠的调脂作用机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1126-1133
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Proteomic-based studies on hypolipidemic mechanism of total phenylpropanoid glycosides from Ligustrum robustum(Roxb.) Blume in hyperlipidemic hamsters
文章编号:
1001-1978(2019)08-1126-08
作者:
张紫文12李晨晨2潘瑞乐2孙 乐2杨润梅2陈爱兵1高南南2
1.河北科技大学化学与制药工程学院,河北 石家庄 050018; 2.中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室&中药(天然药物)创新药物研发北京市重点实验室,北京 100193
Author(s):
ZHANG Zi-wen12 LI Chen-chen2 PAN Rui-le2 SUN Le2 YANG Run-mei2 CHEN Ai-bing1 GAO Nan-nan2
1.College of Chemical and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China; 2.Key Lab of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences,Peking Union Medical College, Beijing 100193, China
关键词:
粗壮女贞总苷 金黄地鼠 高脂血症 label-free蛋白质组学 生物信息学分析 调脂机制
Keywords:
total phenylpropanoid glycosides from Ligustrum robustum(Roxb.)Blume hamsters hyperlipidemia label-free quantitative proteomics bioinformatics analysis hypolipidemic mechanism
分类号:
R-332; R284.1; R341; R349.31; R589.2; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.08.018
文献标志码:
A
摘要:
目的 应用非标记定量(label-free)蛋白质组学技术,探究粗壮女贞总苷(LRTPG)的调脂机制。方法 从高脂血症模型组和LRTPG给药组金黄地鼠肝脏中提取总蛋白,进行label-free蛋白质组学研究。结果 蛋白质组学研究共鉴定出2 231个蛋白质,模型组与LRTPG组共549个差异表达蛋白,其中93种蛋白上调,59种蛋白下调,397种蛋白只在模型组或者给药组中有定量值。GO分析表明,这些差异表达蛋白主要参与代谢、转运、氧化还原、磷酸化、信号转导、脂质代谢等生物学过程。KEGG通路分析表明,这些蛋白集中于氧化磷酸化、非酒精性脂肪肝病、PI3K-Akt、cAMP、cGMP-PKG等多条信号通路。一些差异蛋白与脂质代谢密切相关,包括CD36、PK、HSS、GCK、ApoA I、Acly、FABP5等。结论 LRTPG调脂作用可能与CD36、PK、HSS、GCK、ApoA I、Acly、FABP5有关。
Abstract:
Aim To explore the hypolipidemic mechanism of the total phenylpropanoid glycoside from Ligustrum robustum(Roxb.)Blume(LRTPG)on hyperlipidemic hamsters using label-free quantitative proteomic technique.Methods The total protein was extracted from livers of model group and the group treated with LRTPG for label-free quantitative proteomics research.Results The proteomic data showed that a total of 2231 proteins were identified.And 549 proteins were found to be differentially expressed between model group and group treated with LRTPG.Among the 549 proteins, 93 proteins were up-regulated and 59 proteins were down-regulated, and 397 proteins had quantitative values only in model group or drug-administered group.Further, gene ontology(GO)analysis indicated that those differentially expressed proteins were primarily involved in an array of biological processes including metabolism, transport, oxidation-reduction, phosphorylation, signal transduction and lipid metabolism.KEGG pathway analysis revealed that these proteins were involved in several signal pathways including oxidative phosphorylation, non-alcoholic fatty liver dis-ease, PI3K-Akt, cAMP, and cGMP-PKG pathway.And some of these proteins were much related to the lipid metabolism, such as CD36, PK, HSS, GCK, ApoA I, Acly and FABP5.Conclusion The hypolipidemic effect of LRTPG may be related to CD36, PK, HSS, GCK, ApoA I, Acly and FABP5.

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相似文献/References:

[1]孙 乐,贺震旦,杨润梅,等.粗壮女贞总苷降脂作用及其基于AMPK通路的降脂作用机制研究[J].中国药理学通报,2017,(08):1073.[doi:10.3969/j.issn.1001-1978.2017.08.008]
 SUN Le,HE Zhen-dan,YANG Run-mei,et al.Hypolipidemic activity of total phenylpropanoid glycosides from Ligustrum robustum(Roxb.)Blume and its mechanisms on AMPK pathway[J].Chinese Pharmacological Bulletin,2017,(08):1073.[doi:10.3969/j.issn.1001-1978.2017.08.008]

备注/Memo

备注/Memo:
收稿日期:2019-03-15,修回日期:2019-05-20
基金项目:国家自然科学基金青年基金项目(No 81703746); 北京协和医学院青年科研基金资助项目(No 3332015142)
作者简介:张紫文(1993-),女,硕士生,研究方向:中药药理学,E-mail:13821942110@163.com;
杨润梅(1981-),女,硕士,副研究员,研究方向:中药心脑血管药理学,通讯作者,Tel:010-57833236,E-mail:rmyang@implad.ac.cn;
陈爱兵(1978-),男,博士,教授,博士生导师,研究方向:纳米材料,通讯作者,Tel:0311-88632183,E-mail:chen_ab@163.com
更新日期/Last Update: 2019-08-14