[1]杨泽霖,黄 鑫,刘俊杰,等.红景天苷调控PI3K/Akt信号通路对LPS诱导的BV2小胶质细胞的抗炎作用[J].中国药理学通报,2019,(08):1145-1149.[doi:10.3969/j.issn.1001-1978.2019.08.021]
 YANG Ze-lin,HUANG Xin,LIU Jun-jie,et al.Anti-inflammatory effects of salidroside on LPS-induced BV2 microglia cells via PI3K/Akt signaling pathway[J].Chinese Pharmacological Bulletin,2019,(08):1145-1149.[doi:10.3969/j.issn.1001-1978.2019.08.021]
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红景天苷调控PI3K/Akt信号通路对LPS诱导的BV2小胶质细胞的抗炎作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1145-1149
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Anti-inflammatory effects of salidroside on LPS-induced BV2 microglia cells via PI3K/Akt signaling pathway
文章编号:
1001-1978(2019)08-1145-05
作者:
杨泽霖1黄 鑫1刘俊杰1唐宇恒1洪海棉2南丽红1洪桂祝1赖文芳1
1.福建中医药大学药学院,福建 福州 350122; 2.福建中医药大学附属人民医院药学部,福建 福州 350004
Author(s):
YANG Ze-lin1 HUANG Xin1 LIU Jun-jie1 TANG Yu-heng1 HONG Hai-mian2 NAN Li-hong1 HONG Gui-zhu1 LAI Wen-fang1
1.College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; 2.Dept of Pharmacy, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China
关键词:
红景天苷 BV2小胶质细胞 PI3K/Akt信号通路 抗炎作用 脂多糖 NF-κB
Keywords:
salidroside BV2 microglia cells PI3K/Akt pathway anti-inflammatory effect LPS NF-κB
分类号:
R-332; R284.1; R322.8; R329.25; R364.5; R392.12; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.08.021
文献标志码:
A
摘要:
目的 探讨红景天苷对脂多糖(LPS)诱导的BV2小胶质细胞的抗炎作用及其机制。方法 建立LPS诱导BV2小胶质细胞损伤模型。经不同浓度的红景天苷作用后,qPCR法检测细胞因子IL-6、IL-1β、TNF-α mRNA的表达; Western blot法检测Akt、p-Akt、核蛋白NF-κB p50的蛋白表达。PI3K抑制剂LY294002作用30 min,再经红景天苷作用后,检测Akt、p-Akt、核蛋白NF-κB p50、IL-6、IL-1β、TNF-α等指标。结果 与模型组比较,红景天苷能够抑制BV2小胶质细胞IL-6、IL-1β、TNF-α mRNA的表达,促进p-Akt蛋白表达,抑制核蛋白NF-κB p50; 经LY294002作用后,红景天苷对p-Akt、核蛋白NF-κB p50、IL-6、IL-1β等作用不明显。结论 红景天苷能够抑制LPS诱导的BV2小胶质细胞炎症反应,主要是通过激活PI3K/Akt信号通路,促进Akt的磷酸化,抑制NF-κB p50核转录,进而抑制细胞因子。
Abstract:
Aim To investigate the anti-inflammatory effect of salidroside on lipopolysaccharide(LPS)-induced BV2 microglia and its mechanism.Methods The model of LPS-induced injury in BV2 microglia was established.The expression of cytokines IL-6, IL-1β and TNF-α mRNA was detected by qPCR, and the expression of Akt, p-Akt, nuclear protein NF-κB p50 protein was dectected by Western blot with different concentrations of salidroside treatment.The model was sequentially treated with PI3K inhibitor LY294002 for 30 min, and after the treatment of salidroside, the expressions of Akt, p-Akt, nuclear protein NF-κB p50, IL-6, IL-1β, TNF-α and other indicators were dectected by Western blot.Results Compared with model, salidroside reduced the expression of IL-6, IL-1β and TNF-α mRNA in BV2 microglia, improved the expression of p-Akt protein, and reduced the nuclear protein NF-κB p50.After the treatment of LY294002, salidroside had no obvious effect on the expression of p-Akt, nuclear protein NF-κB p50, IL-6, IL-1β.Conclusions Salidroside can inhibit LPS-induced BV2 microglia inflammatory reaction by activating PI3K/Akt signaling pathway, promoting Akt phosphorylation, inhibiting NF-κB p50 nuclear transcription, and inhibiting cytokines.

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备注/Memo

备注/Memo:
收稿日期:2019-03-20,修回日期:2019-04-24
基金项目:国家自然科学基金资助项目(No 81603323,81473382); 福建中医药大学项目(No X2018002-协同); 2018年度福建省教育厅杰青项目; 福建中医药大学校管课题(No X2018010)
作者简介:杨泽霖(1989-),男,硕士生,研究方向:中药学,E-mail:576635516@qq.com;
赖文芳(1985-),女,博士,助理研究员,研究方向:中药心脑血管药理学,通讯作者,E-mail:laiwenfang08@163.com
更新日期/Last Update: 2019-08-14