[1]王重阳,姜京植,李俊峰,等.隐丹参酮通过TWEAK/Fn14和TGF-β1/Smads信号通路缓解OVA诱导哮喘小鼠气道炎症[J].中国药理学通报,2019,(08):1149-1154.[doi:10.3969/j.issn.1001-1978.2019.08.022]
 WANG Chong-yang,JIANG Jing-zhi,LI Jun-feng,et al.Effects of cryptotanshinone on airway inflammation models in asthmatic mice through TWEAK/Fn14 and TGF-β1/Smads signaling pathways[J].Chinese Pharmacological Bulletin,2019,(08):1149-1154.[doi:10.3969/j.issn.1001-1978.2019.08.022]
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隐丹参酮通过TWEAK/Fn14和TGF-β1/Smads信号通路缓解OVA诱导哮喘小鼠气道炎症()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1149-1154
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Effects of cryptotanshinone on airway inflammation models in asthmatic mice through TWEAK/Fn14 and TGF-β1/Smads signaling pathways
文章编号:
1001-1978(2019)08-1149-06
作者:
王重阳1姜京植1李俊峰1朱莲花2金 珊2金哲虎2徐 畅1延光海1
1.延边大学医学院解剖学教研室,吉林 延吉 133002; 2.延边大学附属医院皮肤科,吉林 延吉 133000
Author(s):
WANG Chong-yang1 JIANG Jing-zhi1 LI Jun-feng1 ZHU Lian-hua2 JIN Shan2 JIN Zhe-hu2 XU Chang1 YAN Guang-hai1
1.Dept of Anatomy, College of Medicine, Yanbian University,Yanji Jilin 133002, China; 2.Dept of Dermatology, Hospital of Yanbian University, Yanji Jilin 133000, China
关键词:
隐丹参酮 气道炎症 细胞因子 肿瘤坏死因子样弱凋亡诱导因子 成纤维细胞生长诱导因子14 转化生长因子β1
Keywords:
cryptotanshinone airway inflammation cytokine TNF-related weak inducer of apoptosis(TWEAK) fibroblast growth factor-inducible 14(Fn14) transforming growth factor β1(TGF-β1)
分类号:
R-332; R284.1; R322.35; R364.5; R392.12; R562.25; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.08.022
文献标志码:
A
摘要:
目的 探究隐丹参酮对哮喘小鼠气道重塑模型的治疗作用,以及其机制是否与TWEAK/Fn14和TGF-β1/Smads信号通路相关。方法 选取♀ BALB/c小鼠40只,分为5组(每组8只),分别为control组、OVA模型组、隐丹参酮低、高剂量组(20、40 mg·kg-1)、地塞米松阳性对照组(1 mg·kg-1)。HE及PAS染色法观察小鼠肺组织的炎症情况及杯状细胞变化; Diff-Quick染色法对小鼠BALF中各类细胞计数; ELISA法检测小鼠BALF中炎性及促炎细胞因子的含量; Western blot检测肺组织TWEAK、Fn14、TGF-β1、Smad2/3、Smad4表达; 免疫组化法检测小鼠肺组织中TWEAK、TGF-β1的表达水平。结果 隐丹参酮能减少OVA诱导的炎症细胞渗出及杯状细胞增生; 隐丹参酮抑制哮喘小鼠BALF中总细胞及EOS、NEU、LYM的生成,并降低促炎细胞因子的水平; Western blot结果显示,隐丹参酮抑制TWEAK、Fn14、TGF-β1、Smad2/3、Smad4的蛋白表达; 免疫组化结果显示,隐丹参酮可减少肺组织中TWEAK、TGF-β1蛋白表达。结论 隐丹参酮通过TWEAK/Fn14和TGF-β1/Smads信号通路改善OVA诱导的小鼠气道炎症。
Abstract:
Aim To explore the therapeutic effect of cryptotanshinone(CTS)on airway remodeling model of asthmatic mice, and the relationship between its mechanism and the TWEAK/Fn14 and TGF-β1/Smads signaling pathway.Methods Forty female BALB/c mice were used for our study, eight mice as a group, and were assigned into five groups, namely, control group, OVA model group, CTS treatment group(20, 40 mg·kg-1), and Dex positive control group(1 mg·kg-1).HE and PAS staining were used to observe lung histopathological changes in mice; Diff-Quick staining was employed to count the types of cells; ELISA was used to detect the contents of proinflammatory cytokines in BALF; Western blot was applied to analyze the contents of TWEAK, Fn14, TGF-β1, Smad2/3, Smad4 in lung tissues; immunohistochemical method was used to detect the expression levels of TWEAK and TGF-β1 in lung tissues.Results CTS reduced the exudation of inflammatory cells and proliferation of goblet cells; CTS inhibited the generation of EOS, NEU, LYM and the total cells; CTS could reduce the level of proinflammatory cytokines of airway inflammation; the results of Western blot showed that CTS inhibited the protein expression of TWEAK, Fn14, TGF-β1, Smad2/3 and Smad4; immunohistochemical results indicated that CTS increased the expression of TWEAK and TGF-β1 in lung tissues.Conclusions CTS has therapeutic effect on the OVA-induced airway inflammation mouse model through TWEAK/Fn14 and TGF-β1/Smads signaling pathways.

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备注/Memo

备注/Memo:
收稿日期:2019-04-11,修回日期:2019-05-20
基金项目:国家自然科学基金资助项目(No 81560679,81860729)
作者简介:王重阳(1991-),女,博士生,研究方向:过敏性疾病,E-mail:15326338995@163.com;
延光海(1975-),男,博士,教授,博士生导师,研究方向:过敏性疾病,通讯作者,E-mail:ghyan@ybu.edu.cn
更新日期/Last Update: 2019-08-14