[1]孙礼宾,朱月琴,潘学胜,等.酸敏感离子通道-1a在急性肺损伤大鼠肺组织中的表达及作用初探[J].中国药理学通报,2019,(08):1155-1159.[doi:10.3969/j.issn.1001-1978.2019.08.023]
 SUN Li-bin,ZHU Yue-qin,PAN Xue-sheng,et al.Expression and role of acid-sensitive ion channel-1a in lung tissues of rats with acute lung injury[J].Chinese Pharmacological Bulletin,2019,(08):1155-1159.[doi:10.3969/j.issn.1001-1978.2019.08.023]
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酸敏感离子通道-1a在急性肺损伤大鼠肺组织中的表达及作用初探()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1155-1159
栏目:
论著
出版日期:
2019-08-14

文章信息/Info

Title:
Expression and role of acid-sensitive ion channel-1a in lung tissues of rats with acute lung injury
文章编号:
1001-1978(2019)08-1155-05
作者:
孙礼宾1朱月琴2潘学胜2王亚男2陈莹瑛3陈 运2刘敬浩2叶 莹2胡成穆2黄 艳2
1.安徽医科大学附属巢湖医院儿科,安徽 合肥 238000; 2.安徽医科大学药学院,安徽 合肥 230032; 3.安徽医科大学校医院,安徽 合肥 230032
Author(s):
SUN Li-bin1 ZHU Yue-qin2 PAN Xue-sheng2 WANG Ya-nan2 CHEN Ying-ying3 CHEN Yun2 LIU Jing-hao2YE Ying2 HU Cheng-mu2 HUANG Yan2
1.Pediatrics, Chaohu Hospital, Anhui Medical University, Hefei 238000,China; 2.School of Pharmacy, Anhui Medical University, Hefei 230032,China; 3.The infirmary of Anhui Medical University, Hefei 230032,China
关键词:
急性肺损伤 酸敏感离子通道-1a 脂多糖 阿米洛利 地塞米松 炎症
Keywords:
acute lung injury acid sensitive ion channel-1a lipopolysaccharide amiloride dexamethasone inflammation
分类号:
R-332; R322.35; R329.25; R343.9; R364.5; R563.8; R983.1
DOI:
10.3969/j.issn.1001-1978.2019.08.023
文献标志码:
A
摘要:
目的 探讨酸敏感离子通道-1a(ASIC1a)在急性肺损伤(acute lung injury,ALI)大鼠肺组织中的表达及可能的作用。方法 SPF级♂SD大鼠32只,随机分为正常组、ALI组、阿米洛利组和阳性药物组。对动脉血进行血气分析,观察肺组织病理学,肺组织称重后计算湿干重比,ELISA检测血清中TNF-α的表达,qPCR和Western blot检测肺组织中ASIC1a的表达。结果 与正常组相比,模型组肺组织损伤明显; 肺组织湿干重比明显升高; TNF-α的表达水平明显升高(P<0.01)。阿米洛利组及阳性药物组肺组织损伤明显减轻; 肺组织湿干重比较模型组明显下降,TNF-α的表达水平明显降低(P<0.01)。免疫组化、qPCR和Western blot结果显示,与正常组相比,模型组ASIC1a的表达量明显升高(P<0.01),阿米洛利组与模型组相比,ASIC1a的表达量明显减少(P<0.01)。 结论 在脂多糖诱导的ALI大鼠肺组织中ASIC1a的表达增加,其可能参与ALI的发生、发展。
Abstract:
Aim To explore the expression and possible effect of acid sensing ion channel-1a(ASIC1a)in lung tissues of rats with acute lung injury(ALI).Methods Thirty-two male Sprague-Dawley(SD)rats were randomly divided into four groups:control group, model group, amiloride group and Dex group.Blood gas analysis of arterial blood, observation of lung histopathology were performed and wet/dry ratio was calculated after weighing of lung tissues, then the expression of TNF-α in serum was detected by ELISA, and the expression of ASIC1a in lung tissues was also detected by qPCR and Western blot.Results Compared with control group, the lung tissue damage was obvious in model group, the wet/dry ratio of lung tissues obviously increased, and the expression level of TNF-α markedly increased(P<0.01).Lung tissue injury was significantly alleviated in amiloride group and Dex group, the wet/dry ratio of lung tissues obviously decreased, and the expression level of TNF-α significantly decreased(P<0.01).Immunohistochemistry, qPCR and Western blot results showed that, compared with control group, the expression of ASIC1a significantly increased in model group(P<0.01), and markedly decreased in amiloride group(P<0.01).Conclusions The expression of ASIC1a increases in acute lung injury induced by lipopolysaccharide, which may be involved in the occurrence and development of ALI.

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备注/Memo

备注/Memo:
收稿日期:2019-03-16,修回日期:2019-04-24
基金项目:安徽省学术技术带头人后备人选科研活动经费资助项目(No 2015H040); 安徽省杰出青年基金(No 1908085J30); 青年拔尖人才支持计划(No 0601037104)
作者简介:孙礼宾(1970-),男,硕士,副主任医师,研究方向:儿童哮喘发病,E-mail:sunlibin1559@163.com;
黄 艳(1979-),女,博士,教授,硕士生导师,研究方向:抗炎免疫药理学,通讯作者,E-mail:aydhy@126.com
更新日期/Last Update: 2019-08-14