[1]靳艳玲,刘国彦,张宇海,等.探讨蛎昆一号制剂对小鼠骨髓抑制的保护作用及其分子机制[J].中国药理学通报,2019,(08):1179-1183.[doi:10.3969/j.issn.1001-1978.2019.08.027]
 JIN Yan-ling,LIU Guo-yan,ZHANG Yu-hai,et al.Protective effect of Likun preparation No.1 on bone marrow suppression in mice and its molecular mechanism[J].Chinese Pharmacological Bulletin,2019,(08):1179-1183.[doi:10.3969/j.issn.1001-1978.2019.08.027]
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探讨蛎昆一号制剂对小鼠骨髓抑制的保护作用及其分子机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年08期
页码:
1179-1183
栏目:
复方药物药理学
出版日期:
2019-08-14

文章信息/Info

Title:
Protective effect of Likun preparation No.1 on bone marrow suppression in mice and its molecular mechanism
文章编号:
1001-1978(2019)08-1179-05
作者:
靳艳玲1刘国彦2张宇海2洪裕玲2刘 磊2李 龙2叶 兰1
1.贵州医科大学基础医学院机能实验室,贵州 贵阳 550025; 2.厦门大学药学院,厦门大学附属中山医院胃肠外科,福建 厦门 361101
Author(s):
JIN Yan-ling1 LIU Guo-yan2 ZHANG Yu-hai2 HONG Yu-ling2 LIU Lei2 LI Long2 YE Lan1
1.Functional Laboratory, Basic Medical College, Guizhou Medical University, Guiyang 550025,China; 2.School of Pharmaceutical Sciences, Xiamen University; Dept of Gastroenterology, Zhongshan Hospital, Xiamen University, Xiamen Fujian 361101,China
关键词:
蛎昆一号制剂 骨髓抑制 5-氟尿嘧啶 炎症 p-JAK2 p-STAT5
Keywords:
Likun preparation No.1 myelosuppression 5-fluorouracil inflammation p-JAK2 p-STAT5
分类号:
R-332; R289.5; R322.2; R446.11; R392.12; R979.1
DOI:
10.3969/j.issn.1001-1978.2019.08.027
文献标志码:
A
摘要:
目的 探讨蛎昆一号制剂对小鼠骨髓抑制损伤的保护作用及机制。方法 60只♂BALB/c小鼠,随机分为正常对照组、5-氟尿嘧啶(5-Fu)模型组、阳性对照组(rhG-CSF)、蛎昆一号制剂低、中、高剂量组。除正常组外,其余各组腹腔注射5-Fu 1次,建立骨髓抑制模型,给药7 d后血常规分析,ELISA检测血清TNF-α、IL-2水平,Western blot测定脾脏组织中p-JAK2、p-STAT5磷酸化水平。结果 给予蛎昆一号制剂后,小鼠腹泻症状减轻,体质量升高(P<0.05),白细胞、血小板和淋巴细胞均升高(P<0.05); ELISA检测显示,蛎昆一号制剂中、高剂量组血清IL-2含量高于模型组(P<0.05),TNF-α含量低于模型组(P<0.05); Western blot检测显示,与模型组比较,蛎昆一号制剂各剂量组小鼠脾脏组织JAK2、STAT5磷酸化水平上调(P<0.05)。结论 蛎昆一号制剂对5-Fu致小鼠骨髓抑制损伤有保护作用,其分子机制可能与蛎昆一号制剂增加JAK2、STAT5磷酸化水平,促进IL-2产生,减少TNF-α分泌有关。
Abstract:
Aim To explore the protective effectof Likun preparation No.1 on bone marrow suppression injury in mice and the possible mechanism.Methods Sixty male BALB/c mice were randomly divided into normal control group, 5-Fu model group, positive control group(rhG-CSF)and Likun preparation No.1 groups with low, medium and high doses.Myocardial inhibition was established by intraperitoneal injection of 5-fluorouracil once.After seven days of administration, blood routine analysis was performed.Serum levels of TNF-α and IL-2 were detected by ELISA.The expression of p-JAK2 and p-STAT5 protein in spleen tissues were determined by Western blot.Results After given the Likun preparation No.1, the symptoms of diarrhea in mice were alleviated, body weight increased(P<0.05), and white blood cells, platelets and lymphocytes also increased(P<0.05).ELISA test showed that the serum level of IL-2 in Likun preparation high-dose group was higher than that in model group(P<0.05), and the TNF-α content was lower than that in model group(P<0.05).Western blot analysis showed that compared with model group, the expression of p-JAK2 and p-STAT5 protein in spleen of mice in Likun preparation group was up-regulated(P<0.05).Conclusions The Likun preparation No.1 has protective effect on 5-Fu-induced myelosuppression in mice.The molecular mechanism may be related to the up-regulation of p-JAK2 and p-STAT5 protein expression, the promotion of IL-2 production and the reduction of TNF-α secretion.

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备注/Memo

备注/Memo:
收稿日期:2019-05-10,修回日期:2019-06-18
基金项目:厦门市海洋经济发展专项资金(No 17GYY001NF01),黔科合字J字[2008]2281
作者简介:靳艳玲(1993-),女,硕士生,研究方向:神经药理学,E-mail:1329629069@qq.com;
叶 兰(1975-),女,博士,副教授,硕士生导师,研究方向:神经药理学,通讯作者,E-mail:469612112@qq.com
更新日期/Last Update: 2019-08-14