[1]曾晓会,卓俊城,谢凯枫,等.二甲双胍治疗Ⅱ型糖尿病大鼠的代谢组学研究[J].中国药理学通报,2019,(09):1212-1220.[doi:10.3969/j.issn.1001-1978.2019.09.007]
 ZENG Xiao-hui,ZHUO Jun-cheng,XIE Kai-feng,et al.Metabonomic study of metformin in type Ⅱ diabetic rats[J].Chinese Pharmacological Bulletin,2019,(09):1212-1220.[doi:10.3969/j.issn.1001-1978.2019.09.007]
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二甲双胍治疗Ⅱ型糖尿病大鼠的代谢组学研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1212-1220
栏目:
论著
出版日期:
2019-09-09

文章信息/Info

Title:
Metabonomic study of metformin in type Ⅱ diabetic rats
文章编号:
1001-1978(2019)09-1212-09
作者:
曾晓会12 卓俊城123 谢凯枫123李钰婷123 占心佾123 陈玉兴12 甘海宁12 黄雪君12 黄丹娥12
1.广东省中医药工程技术研究院,广东 广州 510095; 2.广东省中医药研究开发重点实验室, 广东 广州 510095; 3.广州中医药大学第五临床医学院,广东 广州 510405
Author(s):
ZENG Xiao-hui12ZHUO Jun-cheng123XIE Kai-feng123LI Yu-ting123ZHAN Xin-yi123CHEN Yu-xing12GAN Hai-ning12HUANG Xue-jun12HUANG Dan-e12
1.Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; 2.Guangdong Provincial Key Lab of Research and Development in Traditional Chinese Medicine, Guangzhou 510095,China; 3.Fifth Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China
关键词:
代谢组学 生物标志物 2型糖尿病 二甲双胍 胆汁酸 脂肪酸
Keywords:
metabolic profile biomarker type 2 diabetic melitus metformin bile acids fatty acid
分类号:
R-332; R322.47; R34-33; R347.8; R587.1; R589; R977.15
DOI:
10.3969/j.issn.1001-1978.2019.09.007
文献标志码:
A
摘要:
目的 研究二甲双胍治疗2型糖尿病(type 2 diabetic melitus,T2DM)大鼠的机制。方法 将大鼠随机分为正常组、模型组和二甲双胍组,每组10只。用高糖高脂饲料(HFSD)诱导4周后,肌肉注射链脲佐菌素(STZ)35 mg·kg-1,给药8周后结束实验。采用Triple TOF液质联用仪(UPLC/ESI-TOF-MS),研究二甲双胍对T2DM大鼠血清中代谢物质的影响,并用qPCR寻找其作用靶点。结果 与正常组相比,T2DM模型组大鼠的体质量、胰腺指数和胰岛素水平明显降低(P<0.05),肝脏指数、GLU、TC、TG、FFA水平均升高(P<0.01),OGTT明显受损。二甲双胍能明显升高体质量、胰腺指数和胰岛素水平(P<0.05),降低肝脏指数、GLU、TC、TG和FFA水平(P<0.05),改善OGTT。通过代谢组学,获得17个生物标志物,验证后发现,二甲双胍对胆固醇、脂肪酸、胆汁酸和磷脂的合成和代谢有调节作用。结论 二甲双胍能通过调节胆固醇、脂肪酸、胆汁酸和磷脂的合成和代谢,改善T2DM的脂质代谢紊乱。
Abstract:
Aim To study the mechanism of metformin in the treatment of type 2 diabetic melitus(T2DM)rats.Methods Rats were randomly divided into normal group, model group and metformin group with 10 rats in each group.Four weeks after induction with HFSD, 35 mg·kg-1 STZ was injected intramuscularly, and the experiment was completed after 8 weeks of administration.UPLC/ESI-TOF-MS was used to study the effects of metformin on metabolites in serum of T2DM rats, and qPCR was used to find its target.Results Compared with normal group, the body weight, pancreatic index and insulin level in model group decreased significantly(P<0.05), liver index, GLU, TC, TG and FFA levels increased significantly(P<0.01), OGTT was significantly impaired, while metformin could significantly increase body weight, pancreatic index and insulin level(P<0.05), reduce liver index, GLU, TC, TG and FFA levels(P<0.05), and improve OGTT.After metabonomics, 17 biomarkers were obtained.After verification, metformin was found to regulate the synthesis and metabolism of cholesterol, fatty acids, bile acids and phospholipids.Conclusions Metformin can improve the lipid metabolism disorder of T2DM by regulating the synthesis and metabolism of cholesterol, fatty acids, bile acids and phospholipids.

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备注/Memo

备注/Memo:
收稿日期:2019-05-16,修回日期:2019-06-27
基金项目:国家科技部“重大新药创制”科技重大专项(No 2016ZX09101076,2018ZX09301011-002)
作者简介:曾晓会(1978-),女,博士,主任中药师,硕士生导师,研究方向:中药药理学,Tel:020-83576735,E-mail:277050896@qq.com;
陈玉兴(1971-),男,主任中药师,硕士生导师,研究方向:中药药理学,通讯作者,Tel:020-83576735,E-mail:tcmgdp@126.com
更新日期/Last Update: 2019-08-14