[1]蒙 萍,景临林,何 蕾,等.MitoQ对模拟高原缺氧大鼠脑组织的保护作用[J].中国药理学通报,2019,(09):1221-1226.[doi:10.3969/j.issn.1001-1978.2019.09.008]
 MENG Ping,JING Lin-lin,HE Lei,et al.Protective effects of MitoQ against plateau hypoxia injury on rats[J].Chinese Pharmacological Bulletin,2019,(09):1221-1226.[doi:10.3969/j.issn.1001-1978.2019.09.008]
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MitoQ对模拟高原缺氧大鼠脑组织的保护作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1221-1226
栏目:
论著
出版日期:
2019-09-09

文章信息/Info

Title:
Protective effects of MitoQ against plateau hypoxia injury on rats
文章编号:
1001-1978(2019)08-1221-06
作者:
蒙 萍1景临林1何 蕾1高迎春1李玉芳2马慧萍1
1.中国人民解放军联勤保障部队第九四○医院药剂科全军高原环境损伤防治重点实验室; 2.甘肃省妇幼保健院麻醉手术科,甘肃 兰州 730050
Author(s):
MENG Ping1 JING Lin-lin1 HE Lei1 GAO Ying-chun1 LI Yu-fang2 MA Hui-ping1
1.Dept of Pharmacy, the 940th Hospital of Joint Logistics Support Force of People's Liberation Army, Key Lab of Prevention and Cure for the Plateau Environment Damage of PLA, Lanzhou 730050, China; 2.Gansu Provincial Maternity and Child-care Hospital, Anesthesia Surgery Dept, Lanzhou 730050, China
关键词:
MitoQ 高原缺氧 大鼠 线粒体功能 氧化应激 低压氧舱
Keywords:
MitoQ plateau hypoxia rats mitochondrial function oxidative stress hypobaric oxygen chamber
分类号:
R-332; R322.81; R329.24; R339.54; R442.9; R845.22; R979.9
DOI:
10.3969/j.issn.1001-1978.2019.09.008
文献标志码:
A
摘要:
目的 研究MitoQ对模拟海拔8 000 m高原缺氧大鼠脑组织的保护作用。方法 采用常压密闭缺氧实验考察MitoQ的抗缺氧活性,并确定最佳给药剂量; 采用大型低压氧舱模拟海拔8 000 m高原缺氧环境,检测MitoQ对缺氧大鼠脑组织结构的影响,以及氧化应激及线粒体功能相关指标,同时检测凋亡相关蛋白,对MitoQ对缺氧大鼠的保护作用进行研究。结果 MitoQ使模拟高原缺氧大鼠脑组织细胞间隙减小,空泡化程度降低,明显降低大鼠脑组织MDA含量(P<0.05),降低胞内Ga2+含量(P<0.01),提高线粒体膜电位(P<0.05),在一定程度上提高缺氧条件下线粒体呼吸链复合物Ⅱ、Ⅳ的活性,明显降低Cyt C、caspase-3、Bax的表达(P<0.05,P<0.01),改善缺氧对大鼠脑及其线粒体功能的损伤。结论 MitoQ可以减少模拟海拔8 000 m缺氧环境对大鼠造成的损伤,维持缺氧条件下大鼠脑组织线粒体功能,对缺氧条件下的大鼠发挥保护作用。
Abstract:
Aim To study the protective effects of MitoQ against an altitude of 8 000 m plateau hypoxia injury on brain tissues of rats.Methods We studied the protective effects of MitoQ against hypoxia by the experiment of closed normobaric hypoxia to choose the best dose firstly.Then, an altitude of 8 000 m plateau hypoxia environment was stimulated using a large scale hypobaric and mionectic chamber, the histopathological slices were observed, and the level of oxidative stress and mitochondrial function as well as measurement apoptosis related proteins were determined to study the anti-hypobaric hypoxia effects of MitoQ on rats.Results MitoQ reduced the intercellular space, the degree of vacuolization of brain and oxidative stress level, prevented calcium overload, raised mitochondrial membrane potential and increased the activity of mitochondrial respiratory chain complexes II, IV.Furthermore, MitoQ decreased the expression level of Cyt C, caspase-3 and Bax, playing a protective role under the condition of hypobaric hypoxia on rats.Conclusions MitoQ prevents the damage and maintains the function of mitochondria of brain to protect rats under the condition of simulated altitude of 8 000 m plateau hypoxia environment.

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备注/Memo

备注/Memo:
收稿日期:2019-05-16,修回日期:2019-06-13
基金项目:国家自然科学基金资助项目(No 81571847,81872796); 军队后勤科研计划面上项目(No CWH17J010); 中国博士后科学基金资助项目(No 2016T91005)
作者简介:蒙 萍(1990-),女,硕士,药师,研究方向:抗高原缺氧损伤药物研发,E-mail:mengping35@163.com;
马慧萍(1966-),女,博士,主任药师,研究方向:抗缺氧损伤药物研发,通讯作者,E-mail:1026573411@qq.com;
李玉芳(1965-),女,副主任护师,研究方向:抗缺氧损伤药物研发,通讯作者,E-mail:1026093448@qq.com
更新日期/Last Update: 2019-08-14