[1]张伟云,王明军,刘华欣,等.环氧泽泻烯对2型糖尿病小鼠的降糖作用[J].中国药理学通报,2019,(09):1240-1244.[doi:10.3969/j.issn.1001-1978.2019.09.011]
 ZHANG Wei-yun,WANG Ming-jun,LIU Hua-xin,et al.Hypoglycemic effect of alismoxide in type 2 diabetic mice[J].Chinese Pharmacological Bulletin,2019,(09):1240-1244.[doi:10.3969/j.issn.1001-1978.2019.09.011]
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环氧泽泻烯对2型糖尿病小鼠的降糖作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1240-1244
栏目:
论著
出版日期:
2019-09-09

文章信息/Info

Title:
Hypoglycemic effect of alismoxide in type 2 diabetic mice
文章编号:
1001-1978(2019)09-1240-05
作者:
张伟云1王明军1刘华欣1王 青1陈全成2
1.厦门医学院药学系,福建 厦门 361023; 2.厦门大学药学院,福建 厦门 361102
Author(s):
ZHANG Wei-yun1 WANG Ming-jun1 LIU Hua-xin1 WANG Qing1 CHEN Quan-cheng2
1.Dept of Pharmacy, Xiamen Medical College, Xiamen 361023,China; 2.School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China
关键词:
环氧泽泻烯 降血糖作用 口服葡萄糖耐量试验 2型糖尿病小鼠 3T3-L1前脂肪细胞 分化
Keywords:
alismoxide hypoglycemic effect oral glucose tolerance test type 2 diabetic mouse 3T3-L1 pre-adipocytes differentiation
分类号:
R-332; R284.1; R329.24; R446.11; R587.1; R977.15
DOI:
10.3969/j.issn.1001-1978.2019.09.011
文献标志码:
A
摘要:
目的 探索环氧泽泻烯的降血糖活性。方法 采用链脲佐菌素与烟酰胺建立2型糖尿病小鼠模型。腹腔注射链脲佐菌素与烟酰胺后的d 21测定血糖,d 22进行口服葡萄糖耐量试验。选择罗格列酮作为阳性对照药,每天按小鼠体重以10 mg·kg-1剂量灌胃给药,连续3周; 环氧泽泻烯不同剂量组每天分别按小鼠体重以5、10、20 mg·kg-1剂量灌胃给药,连续3周。此外,采用油红O染色法探索了0.5、1 μmol·L-1环氧泽泻烯对3T3-L1前脂肪细胞分化的影响。结果 环氧泽泻烯不仅使2型糖尿病小鼠空腹血糖下降,而且在口服葡萄糖耐量试验期间也表现出降低血糖的趋势。在0.5、1 μmol·L-1浓度下,环氧泽泻烯促进了3T3-L1前脂肪细胞分化过程。结论 环氧泽泻烯可能具有降血糖活性,加速前脂肪细胞分化过程,其机制有待进一步研究。
Abstract:
Aim To investigate the hypoglycemic effect of alismoxide.Methods Type 2 diabetes mellitus(DM)mouse model induced by combined administration of streptozotocin and nicotinamide was adopted.Three weeks later, blood glucose of blank control group and type 2 diabetic mouse model group was measured on day 21, and oral glucose tolerance test(OGTT)was carried out on day 22, respectively.After type 2 diabetic mouse model was successfully established, rosiglitazone was chosen as positive drug.Oral administration of rosiglitazone at dose of 10 mg·kg-1 daily was performed for three weeks in positive group.Oral administration of alismoxide at dose of 5, 10 and 20 mg·kg-1 daily for three weeks was carried out in alismoxide different dose group, respectively.Furthermore, influence of alismoxide on differentiation was investigated in 3T3-L1 pre-adipocytes, and Oil red O staining was adopted.Results Not only blood glucose was decreased by alismoxide in type 2 DM mice, but also hypoglycemic trend was exhibited during OGTT.Furthermore, at concentration of 0.5 and 1 μmol·L-1, alismoxide promoted 3T3-L1 pre-adipocyte differentiation.Conclusions It suggests that alismoxide might possess hypoglycemic property and accelerate pre-adipocyte differentiation; however, the mechanism involved needs further study.

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备注/Memo

备注/Memo:
收稿日期:2019-05-20,修回日期:2019-06-20
基金项目:国家自然科学基金面上项目(No 81773601); 福建省教育厅中青年教师教育科研项目(No JT180664); 福建省卫生系统项目(No 2015-ZQN-JC-45); 福建省中医药重点研究室项目(No ZD201405)
作者简介:张伟云(1980-),女,博士,副教授,研究方向:中药活性成分及机制, E-mail: zhangwy@xmu.edu.cn;
陈全成(1977-),男,博士,副教授,硕士生导师,研究方向:天然产物活性及作用机制,通讯作者,Tel: 0592-2881189, E-mail: chenqc@xmu.edu.cn
更新日期/Last Update: 2019-08-14